RESUMO
To explore a new method for the therapy of the avascular necrosis of the femoral head, the recombinant plasmid pCD-hVEGF165 was mixed with collagen and was implanted in the necrotic femoral head. The expression of vascular endothelial growth factor (VEGF) was detected by RNA dot hybridization and immunohistochemical method. The repair of the femoral head was observed by histological method. The results showed that the expression of VEGF was detectable in the femoral head treated with VEGF gene. Angiogenesis in these femoral heads was more abundant than the control. Bone repairing was augmented in the femoral head treated with VEGF gene. The results suggest that angiogenesis in bone tissue could be augmented by gene transfection of VEGF and bone repairing would be accelerated accordingly.
Assuntos
Animais , Coelhos , Colágeno , Usos Terapêuticos , Cabeça do Fêmur , Necrose da Cabeça do Fêmur , Terapêutica , Técnicas de Transferência de Genes , Terapia Genética , Implantes Experimentais , Neovascularização Fisiológica , Osteogênese , Plasmídeos , Genética , Distribuição Aleatória , Proteínas Recombinantes , Genética , Fator A de Crescimento do Endotélio Vascular , GenéticaRESUMO
<p><b>OBJECTIVE</b>To explore a new method for the therapy of avascular necrosis of the femoral head.</p><p><b>METHODS</b>The recombinant plasmid pCD-hVEGF165 was mixed with collagen and was implanted in the necrotic femoral head. The expression of vascular endothelial growth factor (VEGF) was examined by RNA dot hybridization and immunohistochemical techniques. Repair of the femoral head was observed by histological and histomorphometric analysis.</p><p><b>RESULTS</b>The expression of VEGF was detected in the femoral head transfected with the VEGF gene. The femoral head transfected with the VEGF gene showed a significant increase in angiogenesis 2 and 4 weeks after gene transfection and a significant increase in bone formation 6 and 8 weeks after gene transfection on histomorphometric analysis (P < 0.01).</p><p><b>CONCLUSIONS</b>Transfection of the VEGF gene enhances bone tissue angiogenesis. Repair of osteonecrosis could be accelerated accordingly, thus providing a potential method for therapy of osteonecrosis.</p>