Analysis of drug use in medical institutions of Guangdong area during 2017-2022 / 中国药房

OBJECTIVE To analyze the situation of drug use in medical institutions of Guangdong area from 2017 to 2022， and to provide reference for the production， sale and procurement of drugs by government departments， medical insurance departments， pharmaceutical enterprises， drug sales enterprises and hospitals. METHODS Using the retrospective analysis method， based on original data from Guangdong hospital information network from 2017 to 2022， statistical analysis was conducted on the drug procurement amount of 118 medical institutions that had been online for the past 6 years， as well as the distribution of 14 major categories of drugs among all purchased drugs by online medical institutions， and sales amount of drug production enterprises. RESULTS The drug procurement amount of 118 medical institutions that had been online in Guangdong area from 2017 to 2022 showed a growth-negative growth-slow growth trend. Among the 14 major categories of drugs， the top 5 drugs in the list of consumption sum were anti-infective drugs， cardiovascular drugs， nervous system drugs， anti-tumor drugs， and digestive system drugs； among the top 200 drugs in terms of procurement amount， three major categories of drugs， namely the anti-infective drugs， anti-tumor drugs and cardiovascular system drugs， occupied the main position. The top 10 drugs in the list of procurement amounts were mainly anti-tumor drugs and cardiovascular system drugs； foreign manufacturers occupied the top 3 places in the list of sales amounts in medical institutions of Guangdong area. CONCLUSIONS In Guangdong area， the growth of drug procurement amount in medical institutions is gradually slowing down， the usage of anti-infective drugs is still abundant， the prevention and treatment of chronic diseases need great attention， and foreign pharmaceutical companies are in a leading position in the sales amount of medical institutions.

Affinity maturation of anti-TNF-alpha scFv with somatic hypermutation in non-B cells

Activation-induced cytidine deaminase (AID) is required for the generation of antibody diversity through initiating both somatic hypermutation (SHM) and class switch recombination. A few research groups have successfully used the feature of AID for generating mutant libraries in directed evolution of target proteins in B cells in vitro. B cells, cultured in suspension, are not convenient for transfection and cloning. In this study, we established an AID-based mutant accumulation and sorting system in adherent human cells. Mouse AID gene was first transfected into the human non-small cell lung carcinoma H1299 cells, and a stable cell clone (H1299-AID) was selected. Afterwards, anti-hTNF-α scFv (ATscFv) was transfected into H1299-AID cells and ATscFv was displayed on the surface of H1299-AID cells. By 4-round amplification/flow cytometric sorting for cells with the highest affinities to hTNF-alpha, two ATscFv mutant gene clones were isolated. Compared with the wild type ATscFv, the two mutants were much more efficient in neutralizing cytotoxicity of hTNF-alpha. The results indicate that directed evolution by somatic hypermutation can be carried out in adherent non-B cells, which makes directed evolution in mammalian cells easier and more efficient.

Targeted deletion of mouse Rad1 leads to deficient cellular DNA damage responses

The Rad1 gene is evolutionarily conserved from yeast to human. The fission yeast Schizosaccharomyces pombe Rad1 ortholog promotes cell survival against DNA damage and is required for G(2)/M checkpoint activation. In this study, mouse embryonic stem (ES) cells with a targeted deletion of Mrad1, the mouse ortholog of this gene, were created to evaluate its function in mammalian cells. Mrad1 (-/-) ES cells were highly sensitive to ultraviolet-light (UV light), hydroxyurea (HU) and gamma rays, and were defective in G(2)/M as well as S/M checkpoints. These data indicate that Mrad1 is required for repairing DNA lesions induced by UV-light, HU and gamma rays, and for mediating G(2)/M and S/M checkpoint controls. We further demonstrated that Mrad1 plays an important role in homologous recombination repair (HRR) in ES cells, but a minor HRR role in differentiated mouse cells.

The Clinical Application of Yashiro Catheter in Chemoembolization of Pelvic Tumors / 实用放射学杂志

Objective To study the clinical application of Yashiro catheter in chemoembolization of pelvic tumors.Methods Yashiro catheters were used in 11 of 23 cases with pelvic tumors who using Cobra catheter could not insert into homolateral internal iliac artery and median sacral artery,the blood supply arteries of tumors were found and selective chemoembolism was performed.The embolism of main stemof bilateral internal iliac arteries were done except the blood supply arteries of tumors with gelfoam in 12 of 23 cases and resection wasperformed by the same surgeons in 24~48 hours after chemoembolization.Results The time of intubatton in one branch of homolateralinternal iliac artery and median sacral artery was obviously shorten with Yashiro catheters.Superselective intubatton was successful in allpatients.The tumors were easy to strip,less bleeding and short operation time during operation.There were not recurrence followed-up 6~18 months after operation.Conclusion Chemoembolization of pelvic neoplasm by Yashiro is an effective and safe technique,it is beneficial to the removal of the pelvic tumor completely and reducing the recurrence of the tumors or metastasis.

The Analysis of Interventional Therapeutic Effect Recently of 47 Cases With Hysteromyoma / 实用放射学杂志

Objective To discuss the curative effect of hysteromyoma by uterine artery embolism.Methods The Seldinger's method was used for uterine artery embolism to treat hysteromyoma in 47 cases.The size of tumor and clinical symptoms were observed 3 and 6 months later after treatment.Results The blood supply of hysteromyoma was from uterine artery branches and mostly were bilateral blood supply style.6 months later after treatment ,the tumors were shrinked and clinical symptoms improved markedly in all cases.Conclusion The recent effect of intervenlional treatment for hysteromyoma is good.Infiltration anesthesia of uterine cavity can distinctly reduce incidence of pelvic cavity pain.

Analysis of the Curative Effects of Interventional Therapy for Adenomyosis in 39 Cases / 实用放射学杂志

Objective To explore the curative effects of uterine arterial embolization(UAE) in treating adenomyosis.Methods Bilateral UAE was performed by Seldinger’s technique in 39 patients with adenomysis (11 cases coexisted with uterine leiomyoma).The catamenia,menorrhalgia,anemia,the size of uterus and lesions were observed after procedure.Results 6 months after treatment,mean catamenia was reduces 56.2%(?