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OBJECTIVE@#This study aimed to investigate the therapeutic effects of Xiaoyao San (XYS), a herbal medicine formula, on exercise capacity and liver mitochondrial metabolomics in a rat model of depression induced by chronic unpredictable mild stress (CUMS).@*METHODS@#A total of 24 male SD rats were randomly divided into four groups: control group (C), CUMS control group (M), Venlafaxine positive treatment group (V), and XYS treatment group (X). Depressive behaviour and exercise capacity of rats were assessed by body weight, sugar-water preference test, open field test, pole test, and rotarod test. The liver mitochondria metabolomics were analyzed by using liquid chromatography-mass spectrometry (LC-MS) method. TCMSP database and GeneCards database were used to screen XYS for potential targets for depression, and GO and KEGG enrichment analyses were performed.@*RESULTS@#Compared with C group, rats in M group showed significantly lower body weight, sugar water preference rate, number of crossing and rearing in the open field test, climbing down time in the pole test, and retention time on the rotarod test (P < 0.01). The above behaviors and exercise capacity indices were significantly modulated in rats in V and X groups compared with M group (P < 0.05, 0.01). Compared with C group, a total of 18 different metabolites were changed in the liver mitochondria of rats in M group. Nine different metabolites and six metabolic pathways were regulated in the liver mitochondria of rats in X group compared with M group. The results of network pharmacology showed that 88 intersecting targets for depression and XYS were obtained, among which 15 key targets such as IL-1β, IL-6, and TNF were predicted to be the main differential targets for the treatment of depression. Additionally, a total of 1 553 GO signaling pathways and 181 KEGG signaling pathways were identified, and the main biological pathways were AGE-RAGE signaling pathway, HIF-1 signaling pathway, and calcium signaling pathway.@*CONCLUSION@#XYS treatment could improve depressive symptoms, enhance exercise capacity, positively regulate the changes of mitochondrial metabolites and improve energy metabolism in the liver of depressed rats. These findings suggest that XYS exerts antidepressant effects through multi-target and multi-pathway.
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Objectives:To investigate the risk factors associated with carotid atherosclerosis based on a retrospective longitudinal monitoring cohort study of healthy subjects during physical examinations.Methods:From 2005 to 2019, a total of 3 344 healthy adults who received at least five routine physical examinations at the Beijing Physical Examination Center and had no carotid atherosclerosis for the first time were selected as the research objects, including 1 714 males (51.25%).The average age of the male and female participants was 47.14 and 47.56 years old, respectively. A retrospective longitudinal monitoring cohort was established and the incidence of carotid atherosclerosis was documented during the follow-up. A Cox regression model was used to analyze the risk factors related to carotid atherosclerosis.Results:At the follow-up of 5.17 years, 2 038 patients had developed carotid atherosclerosis (60.94%, 66.69% in males and 54.91% in females). Multivariate Cox regression analysis showed that advanced age (>60 years old, HR=18.973, 95% CI: 12.353-29.139), female ( HR=0.639, 95% CI: 0.582-0.701), hypertension ( HR=1.166, 95% CI: 1.047-1.298), and low estimated glomerular filtration rate ( HR=1.011, 95% CI: 1.009-1.013) were associated with the onset of carotid atherosclerosis. Conclusions:The main factors influencing the development of carotid atherosclerosis included age, sex, blood pressure, and estimated glomerular filtration rate.
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More and more studies have found that intestinal flora is associated with connective tissue diseases. This review summarizes characteristics of intestinal flora and its mechanism of action in connective tissue diseases, and mainly elaborates correlations between intestinal flora imbalance and 3 common connective tissue diseases (lupus erythematosus, systemic scleroderma and Sjogren′s syndrome) , its possible mechanism of action and related hypothesis. Probiotics can regulate the intestinal flora imbalance, and serve as one of treatments for these common connective tissue diseases.
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Objective To explore the scientific development direction in medical institutions under the new health care reform,and provide a reference for promotion of scientific innovation and development of medical institutions in Beijing.Methods Through analyzing the fund of Natural Science Foundation of China (NSFC) in 2016,scientific competitive power of medical institutions in Beijing was explored from the aspect of project type,subject classification and talent training.Results The NSFC by medical institutions in Beijing mainly focused on medical science department (92.68％),of which corresponding subjects were mainly oncology,neural and mental disease and traditional Chinese medicine.The project approved rates of tertiary hospitals subordinate to country and Beijing were 43.46％ and 29.22％,respectively.Only a few dispersing talent projects were obtained.Conclusions Funds and approved rate of NSFC increased.Medical institutions in Beijing had relatively strong scientific competitive power in the fundamental researches of major and difficult diseases,and traditional Chinese medicine.Fundamental researches,talent training,research team building and international cooperation should be improved in the further.
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The study is to report the establishment of a method of screening the antitumor compounds based on the dynamic bio-response profile of cells to make up for the shortages of conventional end-point tests such as tedious operation and low sensitivity. Based on the principle of electric impedance of cells, the real-time cell electronic sensing (RT-CES) system was used to monitor the effect of epirubicin (EPI), cisplatinum (DDP) and carboplatin (CBP) on the growth of HepG2 cells, with the cell index (CI), half maximal inhibitory concentration (IC50) and detachment curve as evaluation indexes. Meanwhile, cell counting kit-8 (CCK-8) and microscopy were applied for verification. The results showed that CI curve could sensitively real-time profile the inhibitory effect of model drugs on HepG2 cells. The IC50 of EPI, DDP and CBP were 0.53 +/- 0.04, 9.79 +/- 0.26 and 597.00 +/- 3.79 microg x mL(-1), respectively. What's more, the significant differences of detachment curves of the three drugs indicated that their functional mechanisms might be different, this is consistent with the literature. The RT-CES system with non-invasive, label-free and real-time characteristics could be used to monitor the bio-response profile of the three drugs to HepG2 cells, allowing to qualitatively and quantitatively distinguish the antitumor activities of the three drugs, and could be a complementary method for the present screening of antitumor compounds.
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Objective To explore the risk factors of cognitive impairment in rural elderly patients from Changzhi urban. Methods Totally 118 elderly patients were examined with minimental state examination (MMSE). The common data and histories of coronary heart disease,hypertension,diabetes and chronic obstructive pulmonary disease were collected. Results The prevalence of cognitive impairment was 14.4％.Multivariate analysis showed that MMSE scores was associated with age,stroke history,homocysteine and low density lipoprotein(LDL-C) (x2 =21.166,19.323,13.672,3.698,P＜0.01 or P＜0.05),and cognitive impairment was not associated with gender,alive spouse,hypertension,coronary heart disease, diabetes and chronic obstructive pulmonary disease(P＞0.05).Age(OR=0.853,95％ CI:0.765-0.951),stroke history(OR=0.764,95％ CI:0.629-0.929),LDL-C (OR =0.208,95％ CI:0.062-0.701 ) and activities of daily living (ADL)(OR=0.839,95％ CI:0.725-0.972)were independent risk factors of cognitive impairment in rural elderly patients. Conclusions Age,stroke and LDL-C are independent risk factors for rural elderly patients in the community.
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The paper is to report the establishment of a method for quickly evaluating compatibility of Chinese medicines injections. Isothermal titration calorimetry (ITC) was used to evaluate compatibility of Yiqifumai (YQFM) and vitamin C injection (Vc)/5% glucose injection (5% GS). The diversification of Gibbs free energy (deltaG), enthalpy (deltaH) and entropy (deltaS) were used to decide reaction types of colliquefaction procedures of different injections. The reactive profiles were used to determine signs and quantity of heat. And high performance liquid chromatography (HPLC) was used as a supportive method for ITC. Then, feasible binding sites were analyzed based on the information of spatial structures of major compositions. During the colliquefaction procedure of YQFM and Vc, [deltaH] > T[deltaS], so, the reaction is enthalpy-driving. And the reactive profile showed that a big deal of heat was given out during the procedure. Obviously, chemical reactions happened and the major compositions changed. On the other side, the reaction of YQFM mixed with 5% GS was entropy-driving, because [deltaH] < T[deltaS]. The reactive profile showed there was a little heat gave out. So, non-chemical reactions happened, and the major compositions did not change. The conformity existed between the results of ITC and HPLC. ITC could be used to evaluate the compatibility of Chinese medicines injections because of the advantages of ITC, such as real time, fast, sensitive and having more parameters.
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To observe the clinical effects of Depulpin in deciduous teeth. It was suggested that Depulpin was an effective and safe drug for pulp devitalizer in children.
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OBJECTIVE:To establish an RP-HPLC analysis of Carthamin in mice and to study its pharmacokinetics.METHODS:The serum concentration of Carthamin was determined by RP-HPLC.The blood concentration-time curve was established and the main pharmacokinetic parameters were computed.RESULTS:The linear range of Carthamin was 0.558～55.8 ?g?L-1(r=0.999 2),with the lowest limit of detection at 0.005 ?g?L-1Carthamin in vivo assumed two-compartment model and rapid absorption.CONCLUSION:The proposed method is simple,sensitive and reproducible,and it met the standard for pharmacokinetic study.