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[Objective] To explore the application of Da Vinci robot in laparoscopic pyeloplasty treatment for pediatric hydronephrosis and to improve the effect of operation and shorten the operation time effectively.[Methods] A summary and analysis was performed on the intraoperative cooperation about the clinical data of 14 pediatric hydronephrosis cases (16 sides) whom were performed RALP from November 2015 to July 2016.The intraoperative cooperation includes preoperative education,adequate preparation of surgical instruments,ensuring the normal operation of robot system before surgery and also,the proper body positions,the protection for patients,the establishment of aseptic barrier during operation as well as the maintenance of robot after operation.[Results] All procedures were performed via transperitoneal approach.Docking time was shortened from 40 to 20 min,operation time lasted (115± 38) min.One severe case presented with anastomasis stenosis even after PCN dilatation,and was redid dismembered pyeloplasty half a year later and the ultimate result was good.The other cases recovered well and the overall success rate was 93.75％.There were no robot failure caused by improper cooperation and no complications caused by improper nursing among the 14 pediatric hydronephrosis cases (16 sides) in pediatric hydronephrosis operations.[Conclusion]Da Vinci robot performed well in assisting laparoscopic pyeloplasty treatment for pediatric hydronephrosis,for it leaves light surgical trauma and short duration of operation.Adequate preoperative preparation,medical staff's formal professional technical training and professional cooperation team can not only improve the operation efficiency,but also be the key to the success of the operation.
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Objective To investigate the risk factors and preventive measures of liver transplantation in patients with liver cirrhosis complicated with portal hypertension. Methods About 63 patients with cirrhosis and portal hypertension were treated with liver transplantation in our hospital from June 2014 to June 2016. The risk factors were investigated and the corresponding care measures were taken. Results All the 63 patients underwent the operation successfully. The total operation time ranged from 276 to 750 min, averaged (450.52 ± 25.43) minutes. The intraoperative blood loss was 300~5,600 mL, averaged (1693.06 ± 193.24) mL. Intraoperative blood transfusion ranged from 0 to 3,000mL, with an average (1400.50 ± 400.61) mL. Three patients contracted gastrointestinal bleeding after liver implants, which was cured after timely treatment and 5 patients with sacral pressure red tail, improved after treatment. Conclusion Due to the long operation time, bleeding, low temperature, risks of infection and other factors, pre-assessment of the intraoperative risks and relative coping measures are of great significance for the complete of operations.
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Objective To evaluate the feasibility and efficacy of twin grasper combined with endoloops on closing acute stomach perforation.Methods Two experimental porcines of stomach perforation underwent endoscopic closure by twin grasper combined with endoloops.Survival conditions and closure of perforating were observed.Results Mild infection and abdominal adhesions were observed in one week after operation.The nylon rope fell off and an ulcer-like lesion was presented in the closed position.The methylene blue leak test was negative.Histologic examination revealed chronic inflammatory cells infiltration,granulation,fibroplasias,and regenerative mucous membrane crawling toward the center of perforation.Conclusion The technique of twin grasper combined with nylon ropes in closing the acute stomach perforation is feasible and effective.
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Objective To observe the clinical efficacy of acupoint thread embedding in treating simple obesity and its effect on blood lipids and insulin.Method Eighty patients were randomized into a thread embedding group and an acupuncture group. The acupoint thread embedding was given once every week, while acupuncture was given six times a week, both for 8 weeks in total. The body weight, body mass index (BMI), chest circumference, waist circumference, hip circumference, insulin and blood lipids were observed before and after intervention.Result After intervention, body weight, BMI, waist circumference, waist-hip ratio, fasting insulin level, blood lipids, and insulin resistance dropped significantly in both groups (P0.05).Conclusion Acupoint thread embedding can reduce body weight and three circumferences, and modulate glucose and lipid metabolisms, through which approaching weight loss in a healthy way.
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Objective To explore the nursing effect of strengthened psychological intervention on patients with abortion receiving assisted reproductive technology.Methods 180 patients with abortion receiving assisted reproductive technology from January 2009 to December 2011 were chosen as the research object.They were randomly divided into the control group and the observation group with 90 patients in each group.The control group adopted conventional methods of care for nursing,the observation group received strengthened psychological intervention on the basis of the control group.The assessment results of SCL-90 scale and IDA scale before and after five-day nursing were compared between the two groups.Results The scores of SCL-90 scale and IDA scale after five-day nursing in the observation group were lower than those of the control group,there was significant difference.Conclusions Strengthened psychological intervention for abortion patients receiving assisted reproductive technology demonstrated good effect.It can significantly improve adverse psychological state of these patients.
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Objective To investigate the influence of psychological intervention on the physical and psychomotor development of children born of assisted reproductive technology. Methods 329 cases of ART born children in our hospital were set as the experimental group,the parturients were given pertinent psychological intervention.254 cases of children of spontaneous pregnancy were selected as the control group.The two groups were given follow-up for 24 months.Their physical condition at birth and psychomotor development during follow - up period were compared. Results Compared with the control group,length of infants at birth,body mass of the experimental group were significantly lower,and the incidence rate of premature birth,low birth weight,NICU stay during neonatal stage were higher.But there were no differences in psychomotor development between two groups. Conclusions ART born children of 24-month old show normal psychomotor development,but there are still more unfavorable physical factors,therefore,pertinent nursing intervention should be given based upon psychological intervention in clinic.
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Objective To analyze and sort the character of symptom and pathology of 223 HIV/AIDS patients in Yunnan Provine. Methods Sort the clinical periods into groups subject to 223 HIV/AIDS patients who voluntarily accept the consultations and treatments for the first try in Yunnan province, then observe their tongue-appearance accordingly. Results By separately observing the color, shape and aspect of the tongue and tongue-fur which belongs to patient during AIDS period and asymptomatic period, discovered that the pathological tongue-fur appearance of all clinical periods trends to greasy, thereinto, in period of AIDS, 47.45% were white-greasy, 16.06% were yellow-greasy, 8.02% were yellow and thin. In terms of color of tongue, 46.71% of the total samples were light pink and white, 32.12% were red, and 21.17% were purple and gore. In period of asymptomatic, most of the tongue-appearance was light pink and thin white, which obviously exceeded the AIDS period. It indicates that the higher level of immunity, the more normal appearance of tongue can be expected. Group A (200≤CD4
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OBJECTIVES:The effect of water-soluble polymers polyvinylpyrrolidone(PVP) and polyethylene glycol(PEG)on the complexation of drugs with β-cyclodextrin were investigated.METHODS:the thermodynamic properties of drugs in aqueous β-CD solution and in aqueous solut.RESULTS:The maximum solubilization concentration of PVP or PEG appeared from 1.5g.L－1 to 3.0g.L－1.At this low concentration,PVP or PEG had insignificant effect on the viscosity of the aqueous β-CD solution.The solubilising effect ofβ-CD on chloramphenicol was improved by 15% when containing 2.5g.L－1 polyethylene glycol in the solution and on vitamininum B2by 98%when containing 2.5g.L－1 polyvinylpyrrolidone.In aqueous solutions water-soluble polymers were shown to increase the solubilising effect of β-CD on drugs.Additing of PEG or PVP to the aqueous complexation medium resulted in an increased negative enthalpy change,together with an increased negative entropy change.CONCLUSION：Water-soluble polymers were shown to increase the solubilising effect of β-CD on drugs.
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Objective To investigate the effect of insulin combined with Edaravone therapy on delayed cerebral vasospasm (CVS) after experimental subarachnoid hemorrhage (SAH).Methods 40 New Zealand white rabbits were randomly separated into sham-operated group (n=8),SAH group (n=10),insulin group (n=10) and insulin+Edaravone group(n=12).SAH models were made by injecting blood twice into cisterna magna.30 min later of the first blood injected,the insulin 0.2 U/kg was subcutaneous injected in insulin group and insulin+Edaravone group,3 times daily for 7 d;while,the insulin+Edaravone group received Edaravone 0.5 mg/kg (intravenous injected in ear vein) twice a day for 7 d.7 d after the model made,the basilar artery cross sectional area was measured and the pathological changes were obseved to estimate the degree of CVS.The expression of insulin receptor(InRa) in vascular endothelial cell was detected by immunohistochemical staining.Results The basilar artery cross sectional area between insulin+Edaravone group[(0.46?0.3)mm2]and the sham-operated group[(0.48?0.4)mm2] was no significant difference;but they were obviously bigger than those in SAH group[(0.25?0.3) mm2] and the insulin group [(0.30?0.3)mm2](all P
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The free amino acid concentrations in serum were determined in 65 normal adults by use of the physiological fluid analysis in Hitachi 835-50 Model Amino Acid Autoanalyer. 29 specimens were determined by physiological fluid analysis and protein hydrolyzate analysis method simultaneous-ly.The results of Serine, Glycine, Valine, Isoleucine, Leucine, Tyrosine, Phenylalanine, Lysine, Tryptophan, Proline, and the molar ratio of the bran-ched-chain amino acids/aromatic amino acids were very similar. By hy-drolyzate method Aspartic acid was not detected and Threonine, Glutmic acid, Cystine, Methionine and Arginine were higher than physiological fluid analysis.The reasons for such higher values were discussed.
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In simplifying the method of determination of activation coefficients (AC) of blood glutathione reductase (BGR), we used saponin solution to prepare blood hemolysate instead of repeated freezing and thawing, thus the time of estimation was shortened. 5, 5'-Dithio-bis-2-nitrobenzoic acid (DTNB) was used to determine the reduced glutathione (GSH), the end product of the NADPH-dependent glutathione reductase reaction on the substrate, oxidized glutathione (GSSG), by means of a photoelectric colorimeter instead of alloxan and an ultra-violet spectrophotometer. From the data obtained in animals and men, the AC values determined by the two methods actually showed no difference.50 male rats, weighing 65-75 grams, were divided from the same litters into 6 groups, namely, the deficient, 2, 5, 10, 30?g and the normal group. The experimental period was 9 weeks. According to the growth weights, the riboflavin contents of urine and liver of the animals, the riboflavin nutritional status of different groups was evaluated. Referring to the corresponding AC values of all groups at the 9th week, the standards of AC values for assessing ribcflavin nutriture could be defined as: 80 severely deficient.
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From a study of the experimental riboflavin depletion in rats, it was demonstrated that the activation coefficients (AC) of the blood glutath-ione reductase (BGR) could be used to assess the riboflavin nutritional status of the animal. One week after deficiency the AC values were elevated, while after repletion of riboflavin they returned to normal. The change of AC values appeared sooner than that of daily excretions of riboflavin in urine. So AC value of BGR seemed to be a sensitive index in detecting early riboflavin deficiency. The method of the determination of AC value of BGR was modified so that only 0.02 ml. of whole blood was needed. It is rather convenient when used in field nutritional surveys.