Expression of human vitamin D3 receptor in Ecoli

The ability to produce large quantities of receptor is important for understanding certain fundaments mechanisms of action of the vitamin D3 receptor [VDR]. Conventional receptor isolation techniques can provid only small quantities of purified receptor. The method presented in this study utilizes a ubiquitin fusion technology and a low temperature [< 30 §C] nalidixic acid induction of the lambda PL promoter. Using this nove method, we have overexpressed the biologically active form of human vitamin D3 receptor [hVDR] in E. coli