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Chinese Journal of Biotechnology ; (12): 1068-1073, 2010.
Artigo em Chinês | WPRIM | ID: wpr-292170

RESUMO

On the basis of successful cloning the full length hemagglulinin (HA) and neuramidinase (NA) gene and sequence analysis of influenza virus H1N1, part of the gene was ligated into pMETA. Expression vectors pMETA/HA (52-1 557 bp) and pMETA/NA (121-1 263 bp) were constructed and expressed in pMAD16 induced by methanol. Recombinant protein was purified through Ni2+ affinity chromatography. Western blotting and ELISA were used to determine the antigenic activity of the recombinant protein. SDS-PAGE showed that the recombinant capsid gene could be overexpressed in Pichia methanolica. ELISA and Western blotting showed that the recombinant protein had antigenicity.


Assuntos
Clonagem Molecular , Vetores Genéticos , Genética , Hemaglutininas , Genética , Vírus da Influenza A Subtipo H1N1 , Genética , Neuraminidase , Genética , Pichia , Genética , Metabolismo , Proteínas Recombinantes , Genética , Alergia e Imunologia
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