RESUMO
OBJECTIVE:To establish a HPLC method for the determination of aristolochic acidⅠand aristolochic acid Ⅱin human serum.METHODS:The separation was performed on C 18 column(250mm?4.6mm,5?m).The mobile phase consisted of methanol-water-acetic(72∶27∶1)at a flow rate of1.0ml/min.The detection wavelength was250nm.RESULTS:The linear ranges for aristolochic acidⅠand aristolochic acid Ⅱ were0.84～13.50?g/ml(r=0.9997,n=5)and2.03～32.50?g/ml(r=0.9994,n=5),respectively.The lowest determination concentration of aristolochic acid Ⅰ and aristolochic acid Ⅱ were0.3?g/ml and0.1?g/ml,respectively.CONCLUSION:The method is simple and practicable,and it can provide basis for clinical diagnosis and treatment of aristolochic acid nephropathy.
RESUMO
OBJECTIVE： A RP－ HPLC method was established for determination of serum concentration of propofol in man METHODS： The mobile phase consisted of methanol－ water（ 68∶ 32v/v） The detection was carried out at wave length 258nm and flow rate 1ml/min with carbamazepine as internal standard RESULTS： The retention times of propofol and carbamazepine were 9 20 and 5 16 min， respectively The mean recovery of propofol was 99％ The within－ day and inter－ day variations were all less than 10％ Propofol and carbamazepine were seperated well The assay linearity was obtained in the range of 1～ 16μ g/ml in serum（ r=0 9 994） CONCLUSION： The method is sensitive， simple and reliable for the determination of propofol concentration
RESUMO
Objective To investigate the pharmacokinetics of propofol in children of different ages after a single dose. Methods Thirty-five ASA Ⅰ or Ⅱ children were divided into 3 age groups: group A
RESUMO
OBJECTIVE:A RP-HPLC method was established for determination of serum concentration of propofol in man METHODS:The mobile phase consisted of methanol-water(68∶32v/v)The detection was carried out at wave length 258nm and flow rate 1ml/min with carbamazepine as internal standard RESULTS:The retention times of propofol and carbamazepine were 9 20 and 5 16 min,respectively The mean recovery of propofol was 99% The within-day and inter-day variations were all less than 10% Propofol and carbamazepine were seperated well The assay linearity was obtained in the range of 1～16?g/ml in serum(r=0 9 994) CONCLUSION:The method is sensitive,simple and reliable for the determination of propofol concentration
RESUMO
OBJECTIVE:To establish a method for quick content determination of clobetasol propionate in temeifu pigmentum.METHODS:UV spectrophotometry was used,and the determination was performed at the wavelength of 240nm.RES-ULTS:The detectable concentration of clobetasol propionate have a good linear relationship with its absorbability in the range of 12～18?g/ml.The inter-day and intra-day average recoveries were 99.87%(RSD=1.21%) and 99.79%(RSD=1.01%),respectively.CONCLUSIONS:The present method is rapid and accurate,which can be widely used in small hospitals not equipped with high performance liquid chromatography.