Combined treatment with myo-inositol and luteolin selectively suppresses growth of human lung cancer A549 cells possibly by suppressing activation of PDK1 and Akt / 南方医科大学学报

OBJECTIVE@#To study the effects of myo-inositol and luteolin on human lung cancer A549 cells and explore the possible mechanisms.@*METHODS@#A549 cells were treated with different concentrations of myo-inositol and luteolin, either alone or in combination, and the cell viability was examined using MTT assay. A549 cells and human bronchial epithelial Beas-2B cells were treated for 48 h with 10 mmol/L myo-inositol and 20 μmol/L luteolin, alone or in combination, and the cell proliferation was detected using MTT assay; the colony formation and migration of the cells were examined with colony formation assay and wound healing assay, respectively. The protein expression levels in A549 cells were detected using Western blotting.@*RESULTS@#Both myo-inositol and luteolin could dose-dependently inhibit the viability of A549 cells. Treatments with 10 mmol/L myo-inositol, 20 μmol/L luteolin, and both for 48 h caused significant reduction in the cell viability (92%, 83% and 70% of the control level, respectively) and colony number (79%, 73% and 43%, respectively), and significantly lowered the wound closure rate (24.61%, 13.08% and 8.65%, respectively, as compared with 29.99% in the control group). Similar treatments with myoinositol and luteolin alone or in combination produced no significant inhibitory effect on the growth, colony formation or migration of Beas-2B cells. The expressions of p-PDK1 and p-Akt in myo-inositol-treated A549 cells and the expression of pPDK1 in luteolin-treated cells were significantly decreased ( < 0.05), and the decrements were more obvious in the combined treatment group ( < 0.05).@*CONCLUSIONS@#Luteolin combined with myo-inositol can selectively inhibit the proliferation and migration of A549 cells, and these effects are probably mediated, at least in part, by suppressing the activation of PDK1 and Akt.

Vascular endothelial growth factor gene polymorphism and Stroke / 国际脑血管病杂志

The incidence of stroke increases year by year.It seriously affects the quality of life in patients.The pathogenesis of stroke is related to a variety of factors,involving genetic polymorphisms,biochemical mechanisms,and inflammatory effect.Among them,vascular endothelial growth factor (VEGF) has become one of the hotspots of research on the pathogenesis of stroke in recent years.This article reviews the correlation between VEGF gene polymorphism and stroke.

Clinical significance of detecting t(11;14) by fluorescence in situ hybridization for the diagnosis of 7 patients with atypical mantle cell lymphoma / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To study the clinical features and diagnosis of 7 patients with atypical mantle cell lymphoma (MCL).</p><p><b>METHODS</b>The 7 MCL patients were misdiagnosed as chronic lymphocytic leukemia (CLL) due to a score of 4 for their immunophenotypes. The clinical features and diagnosis of such patients were retrospectively analyzed.</p><p><b>RESULTS</b>Six patients had superficial lymphadenectasis but their lymph nodes could not be palpated. All 7 patients were as stage IV considering bone marrow infiltration. Scores of immunophenotype of CLL were 4, and interphase fluorescence in situ hybridization (FISH) for t(11;14) were positive in all patients.</p><p><b>CONCLUSION</b>Some MCL patients have clinical features similar to CLL. Interphase FISH can play an important role in the diagnosis of MCL.</p>

Primary cardiac plasmablastic lymphoma: report of a case and literature review / 中华血液学杂志

<p><b>OBJECTIVE</b>To report a case of primary cardiac plasmablastic lymphoma to investigate its clinical feature, diagnosis, therapy and prognosis.</p><p><b>METHODS</b>A case of primary cardiac plasmablastic lymphoma was studied. The imaging examination, conventional histopathological and immunohistochemical staining of this case were detected. The clinical feature, pathogenesis, diagnosis, therapy and prognosis of primary cardiac plasmablastic lymphoma were further investigated through literatures review.</p><p><b>RESULTS</b>The tumor was located in the right atrium. Microscopic examination showed diffuse proliferation of large lymphoid cells. The neoplastic cells were positive for CD38 and CD79a. The patient was treated with chemotherapy combined with autologous stem cell transplantation.</p><p><b>CONCLUSION</b>Primary cardiac plasmablastic lymphoma was extremely rare. Its pathogenesis remained to be unclear. With non- specific clinical manifestations, the diagnosis was mainly confirmed by histopathological and immunohistochemical staining method. Without standard treatment, more patients were treated with chemithreapy regimens similar to the treatment used in aggressine lymphoma. Patients usually had a poor prognosis.</p>

Chronic lymphocytic leukemia with central nervous system invasion: one case report and literature review / 中华血液学杂志

<p><b>OBJECTIVE</b>To investigate clinical features, diagnosis, therapy, and prognosis in a case of chronic lymphocytic leukemia (CLL) with central nervous system (CNS) invasion.</p><p><b>METHODS</b>The cellular morphology of bone marrow, cytology and flow cytometry of cells in CSF, and radiographic manifestations were detected in a patient diagnosed as CLL with CNS invasion. The clinical features, pathogenesis, diagnosis,therapy, and prognosis of CLL with CNS invasion were reviewed by literatures.</p><p><b>RESULTS</b>A clonal population of B lymphocytes with typical characteristics of immunophenotype was identified in the patient. After treated with intrathecal chemotherapy, the patient returned to normal cells count of cerebrospinal fluid and normal neurologic status. Previous studies also have demonstrated the efficacy of intrathecal chemotherapy.</p><p><b>CONCLUSION</b>CNS invasion is a rare complication of CLL. Early detection and treatment may result in better outcomes.</p>

Distribution of lymphomas subtypes in Jiangsu Province: a multicenter analysis of 5 147 cases / 中华血液学杂志

<p><b>OBJECTIVE</b>To investigate the epidemiological characteristics of lymphoma in Jiangsu province.</p><p><b>METHODS</b>A total of 5 147 consecutive lymphoma samples collected from 18 hospitals in Jiangsu province from January 2007 to December 2013 and diagnosed according to the WHO classification were enrolled in this study. Basic epidemiological information including age, gender and lymphoma subtypes was analyzed.</p><p><b>RESULTS</b>The median age of all lymphoma cases was 59(2-96) years, and gender ratio (M/F) was 1.6:1. The subtypes distribution analysis revealed that Hodgkin lymphoma (HL) accounted for 5.19% (n=241), whereas non-Hodgkin's lymphoma (NHL) accounted for 94.81% (n=4 400). Further analysis displayed B-NHL formed 75.44% (n=3 501) of all cases and T/NK-NHL 16.51% (n=766), diffuse large B-cell lymphoma and NK/T-cell lymphoma were the major subtypes of B-NHL and T/NK-NHL (53.50%, 1 873/3 501 and 31.85%, 244/766), respectively.</p><p><b>CONCLUSION</b>Unique epidemiological characteristics of lymphoma in Jiangsu province was different from other regions in China and western country, which can provide strong theoretical basis for public health, clinical and basic research.</p>