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AIM: To investigate the mechanism and reversal effect of Zuo Jin Wan (ZJW) on cetuximab (CET) resistance in KRAS mutant colorectal cancer cell. METHODS: The mutation status of KRAS gene in SW620, Lovo, HCT116, HT29 and Caco2 cells were detected by Sanger sequencing. CCK-8 assay was used to detect the effects of ZJW, CET, ZJW combined with CET and CET, ZJW in combination with other cell death inhibitors on the survival rate of the above cells, and to observe the reversal effects of ZJW on CET-treated KRAS mutant cells (SW620, Lovo and HCT116). Flow cytometry, colorimetric method, and Fe
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Objective To explore the correlation between cardiac function classification and homocysteine (HCY) ,N-terminal pro-brainnatriureticpeptide(NT-proBNP),fibrinogen(Fbg),D-dimer(D-D) in patients with chronic pulmonary heart disease (CPHD) .Methods A total of 135 cases of CHPD were divided into 4 groups by cardiac function classification of New York Heart Association(NYHA):NYHA class Ⅰ group(group A ,33 cases) ,NYHA class Ⅱ group(group B ,39 cases) ,NYHA class Ⅲ group (group C ,36 cases) ,NYHA class Ⅳ group(group D ,27 cases) .Meanwhile 50 healthy subjects were recruited into HC group .The level of HCY ,NT-proBNP ,Fbg and D-D were detected and compared between different groups .Receiver operating characteristic (ROC) curves were drawn ,the area under ROC curves(AUCs) were calculated to assess the diagnostic value of each item ,the cor-relation between cardiac function classification and HCY ,NT-proBNP ,Fbg ,D-D in CHPD was analyzed .Results Compared with HC group ,the level of HCY ,Fbg and D-D in group A didn′t rose significantly(P>0 .05) .The level of HCY ,NT-proBNP ,Fbg and D-D in group B ,C ,D were significant with those of the control group(P<0 .05) .NT-proBNP increased higher in CHPD as the du-ration of cardiac function classification of NYHA processed (P<0 .05) .The AUCs of HCY ,NT-proBNP ,Fbg ,D-D were respective-ly 0 .710 ,0 .919 ,0 .782 ,0 .864 in CHPD patients .There were significant correlations between cardiac function classification of NY-HA and HCY ,NT-proBNP ,Fbg ,D-D(P< 0 .01) .Conclusion The level of HCY ,NT-proBNP ,Fbg ,D-D could be considered as monitoring and diagnostic indexes of CHPD .
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Objective To explore the correlation between cardiac function classification and homocysteine (HCY) ,N-terminal pro-brainnatriureticpeptide(NT-proBNP),fibrinogen(Fbg),D-dimer(D-D) in patients with chronic pulmonary heart disease (CPHD) .Methods A total of 135 cases of CHPD were divided into 4 groups by cardiac function classification of New York Heart Association(NYHA):NYHA class Ⅰ group(group A ,33 cases) ,NYHA class Ⅱ group(group B ,39 cases) ,NYHA class Ⅲ group (group C ,36 cases) ,NYHA class Ⅳ group(group D ,27 cases) .Meanwhile 50 healthy subjects were recruited into HC group .The level of HCY ,NT-proBNP ,Fbg and D-D were detected and compared between different groups .Receiver operating characteristic (ROC) curves were drawn ,the area under ROC curves(AUCs) were calculated to assess the diagnostic value of each item ,the cor-relation between cardiac function classification and HCY ,NT-proBNP ,Fbg ,D-D in CHPD was analyzed .Results Compared with HC group ,the level of HCY ,Fbg and D-D in group A didn′t rose significantly(P>0 .05) .The level of HCY ,NT-proBNP ,Fbg and D-D in group B ,C ,D were significant with those of the control group(P<0 .05) .NT-proBNP increased higher in CHPD as the du-ration of cardiac function classification of NYHA processed (P<0 .05) .The AUCs of HCY ,NT-proBNP ,Fbg ,D-D were respective-ly 0 .710 ,0 .919 ,0 .782 ,0 .864 in CHPD patients .There were significant correlations between cardiac function classification of NY-HA and HCY ,NT-proBNP ,Fbg ,D-D(P< 0 .01) .Conclusion The level of HCY ,NT-proBNP ,Fbg ,D-D could be considered as monitoring and diagnostic indexes of CHPD .
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Objective To explore the diagnostic value of serum ischemia‐modified albumin (IMA) ,lipoprotein‐associated phos‐pholipase A2 (Lp‐PLA2) and cardiac troponin I (cTnI) in acute coronary syndrome (ACS) .Methods The levels of serum IMA , Lp‐PLA2 and cTnI were detected in 138 cases of ACS ,including 79 cases of acute myocardial infarction (AMI) ,59 cases of unstable angina pectoris (UAP) ,and the detection results were compared with those in 50 individuals undergoing physical examination(con‐trol group) .The levels of serum IMA ,Lp‐PLA2 and cTnI were compared among various groups .The receiver characteristic curve (ROC) was drawn to evaluate the diagnostic value of IMA ,Lp‐PLA2 and cTnI in ACS .Results The levels of serum IMA ,Lp‐PLA2 and cTnI in patients with UAP and AMI were higher than those in the healthy control group ,and there was statistical differ‐ence between them(t=7 .286 ,8 .589 ,8 .952 ,P<0 .01) .Comparing with the UAP group ,the levels of serum IMA ,Lp‐PLA2 and cTnI in the AMI group were significantly increased(t=4 .063 ,5 .987 ,2 .062 ,P<0 .05) .In the ROC curve analysis ,the area under ROC curve (AUC) of IMA ,Lp‐PLA2 and cTnI were 0 .821 ,0 .843 and 0 .852 respectively .Conclusion The levels of serum IMA , Lp‐PLA2 and cTnI can be used for monitoring and early diagnosis of ACS .