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1.
Chinese Journal of Preventive Medicine ; (12): 99-102, 2005.
Artigo em Chinês | WPRIM | ID: wpr-299190

RESUMO

<p><b>OBJECTIVE</b>To elucidate the potential molecular mechanism responsible for the early time of tumor promotion, gene expression profile was studied in the transformed BALB/c 3T3 cells induced by 12-O-tetradecanoylphorbol-13-acetate (TPA).</p><p><b>METHODS</b>The two-stage cell transformation model was established by using the initiator of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and promoter of TPA. Cell proliferation was measured by trypan blue staining and cell cycle analysis was carried out by flow cytometry assay. A cDNA microarray representing 1 152 genes was used to investigate the gene expression profiles of BALB/c 3T3 cells exposed to TPA at 4 h and 24 h respectively.</p><p><b>RESULTS</b>TPA could effectively inhibit cell proliferation and induce the G1 and S cell cycle arrested in the early time. Moreover 19 genes were found differentially expressed at least twofold in the TPA treated cells as compared with the control cells, 9 of them were upregulated and 10 downregulated. Most of the differentially expressed genes were involved in cell proliferation, differentiation or apoptosis, and related to ras or p53 signal transduction pathway.</p><p><b>CONCLUSION</b>TPA could influence the transcriptional expression of some genes related to cell cycle modulation and ultimately result in the cell growth arrest.</p>


Assuntos
Animais , Camundongos , Apoptose , Genética , Células 3T3 BALB , Ciclo Celular , Genética , Diferenciação Celular , Genética , Proliferação de Células , Transformação Celular Neoplásica , Genética , Citometria de Fluxo , Expressão Gênica , Perfilação da Expressão Gênica , Metilnitronitrosoguanidina , Farmacologia , Análise de Sequência com Séries de Oligonucleotídeos , Métodos , Acetato de Tetradecanoilforbol , Farmacologia
2.
Chinese Journal of Biotechnology ; (12): 703-708, 2002.
Artigo em Chinês | WPRIM | ID: wpr-256135

RESUMO

Phycoerythrocyanin(PEC) lyase-isomerase PecE/PecF from Mastigocladus laminosus is the specific enzyme for biosynthesis of PEC alpha-subunit(alpha-PEC). In this work, the specificity of PecE/PecF on substrate apoproteins was reported. PecE/PecF could catalyse the reconstitution of phycocyanobilin(PCB) with apoproteins of alpha-PEC from two different subspecies of Mastigocladus laminosus, as well the site-directed mutated apoprotein of alpha-PEC with Trp at 128 to Phe in vitro, but could not catalyse the reconstitution of PCB with apoprotein of phycocyanin alpha-subunit(alpha-CPC) from Mastigocladus laminosus. The surfactant Triton X-100 had no effect for the reconstitution of alpha-PEC, while it could improve the reconstitution of PCB with apoprotein of alpha-CPC.


Assuntos
Apoproteínas , Metabolismo , Proteínas de Bactérias , Catálise , Cianobactérias , Complexos de Proteínas Captadores de Luz , Liases , Metabolismo , Octoxinol , Farmacologia , Proteínas , Metabolismo , Especificidade por Substrato
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