Study on triterpenes from Solanum torvum / 中国中药杂志

<p><b>OBJECTIVE</b>To study on the chemical constituents of Solanum torvum.</p><p><b>METHOD</b>The aerial parts of S. torvum swartz were extracted with 95% alcohol and the constituents were isolated and purified by chromatographic silica gel and Sephadex LH-20. Their structures were determined by NMR and MS spectral analysis.</p><p><b>RESULT</b>Six triterpenes were isolated and identified as 3beta-acetyloleanolic acid (1), 3-O-acetyl-11alpha, 12alpha-epoxy-oleanan-28, 13beta-olide (2), oleanolic acid (3), ursolic acid (4), 2alpha-hydroxy-oleanolic acid (5), 2alpha, 3beta-dihydroxyursolic acid (6).</p><p><b>CONCLUSION</b>All compounds were obtained from this plant for the first time.</p>

Determination of Residues of Organochlorinated Pesticides in Polygonum multiflorum by GC-MS with Solid-phase Extraction / 中国药房

OBJECTIVE:To determine the residues of 9 kinds of organochlorinated pesticides in Polygonum multiflorum.ME-THODS:Samples were extracted with ethyl acetate by ultrasonic wave assistant extraction and purified on Florisil solid-phase extraction column.The residues of organochlorinated pesticides were determined with capillary gas chromatography with GC-MS.The separation was performed on DB-5MS(30 m?0.25 mm,0.25 ?m) fused-silica capillary column with injector temperature of 230 ℃.The initial column temperature was 100 ℃,then raised to 220 ℃ at the rate of 8 ℃?min-1.The column temperature was ra-ised at 250 ℃ at the rate of 5 ℃?min-1 for 10 min.The column flow was 1 mL?min-1 and injection volume was 1 ?L.RESULTS:9 kinds of organochlorinated pesticides were completely separated within 30 minutes.The average recoveries ranged from 80.4% to 97.2%(RSD ranged from 3.5% to 7.4%,n=6).CONCLUSION:The method is sensitive and accurate for the content determination of the residues organochlorinated pesticides.

Determination of Anabasine in Fengshiding Tablets by RP-HPLC / 中药新药与临床药理

Objective To determine the content of anabasine in Fengshiding tablets.Methods RP-HPLC method was established.Diamonsil-C18 was used as chromatographic column,the mobile phase consisted of methanol and 0.1 %triethylamine(5 ∶95,adjust pH to 3.6 with H3PO4),the flow rate was 1.0 mL?min-1 and the detection wavelength at 259 nm.Results The average recovery for Anabasine was 99.0 %,RSD=2.8 %(n=6).Conclusion The method can be used to determine the content of anabasine in Fengshiding tablets.