RESUMO
OBJECTIVE: To study the secondary metabolites of sponge-associated fungus Beauveria feline SX-6-22. METHODS: The compounds were isolated and purified by silica gel, ODS reversed-phase column, preparative HPLC techniques. And their structures were identified by the physicochemical properties and NMR, MS data analysis. X-ray crystal diffraction was used to confirm the absolute configuration of compound1. RESULTS: Nine secondary metabolites were isolated from the extract of the solid fermentation culture of strain SX-6-22, including four new compounds beauverins A-D (1-4) and five known compounds 1, 9-dimethyl-3, 7-dibenzofurandiol (5), diorcinol(6), cordyol C(7), mixture of violaceol (8) and violaceol Ⅱ(9). Compounds 5, 7, 8 and 9 showed strong antioxidant activity with IC50 (9.62±0.12), (11.11±0.02), and (8.44±0.08) μg•mL-1 by DPPH assay. CONCLUSION: Four new compounds and three antioxidant known compounds are isolated from sponge-associated fungus SX-6-22.