Association of total leukocytic count and differential leukocytic count as bioinflammatory markers with the glycemia of animal model of diabetes

To measure the White blood counts [TLC and DLC] and see their association with the glycaemic status [fasting blood glucose, glycosylated Hb] of normal and diabetic rats. This experimental study was carried out at Animal house / National Reference Laboratory for Poultry Diseases [NRLPD], Animal Division of National Agriculture Research Centre [NARC] Islamabad, Department of Biochemistry and Molecular Biology Army Medical College Rawalpindi from December 2006 to January 2007. A total of 60 albino rats [30 normal and 30 diabetic] were included in the study. Leucocytic counts were done by the visual method. Care was observed not to count clumped red cell debris. Blood glucose levels were determined by applying glucose oxidase method and the determination of total HbAlc was done using its Diagnostic kit. Significant difference was observed in the values of fasting glucose [p<0.05] but no significant difference was present in the values of Glycosylated Hb [HbAlc] [p>0.05] after experimentation. Comparison of total leukocytic count in normal and diabetic groups [p>0.05] revealed no significant difference. There was also no significant difference found in the values of Neutrophils and Lymphocytes counts [p>0.05]. Because of inadequate values of monocytes, Eosinophils and Basophils in both groups; the statistical analysis revealed no significant results. In a rat model of diabetes mellitus, hyperglycaemia of short duration has no role in producing inflammation as is indicated by no rise in the levels of otal leukocytic counts [TLC and DLC]

Association of c-reactive protein as an inflammatory marker with the glycaemic status of the alloxan treated diabetic rats

To measure the level of C-reactive protein and find its association with the glycaemic status [fasting plasma glucose, glycosylated haemoglobin] of metabolically normal and diabetic albino rats. Total 60 Albino rats were included into [metabolically normal rats n=30; diabetic rats n =30]. Plasma glucose levels were determined by using glucose oxidase method while determination of total Hb and glycosylated hemoglobin [HbA[1]c] was done by diagnostic kit that uses weak cation-exchange resin to bind Hb. The% HbA[1]c was determined by measuring the absorbance at 415 nm of the glycosylated hemoglobin fraction and the total hemoglobin fraction. The ratio of the two absorbances gave the% of HbA[1]c. C-reactive protein was measured by the ELISA kit. Significant difference was found in the values of fasting glucose, of the normal and diabetic groups [p<0.05] but no significant difference was present in the values of HbA[1]c of both groups. There was no significant difference in the values of C-reactive protein of the normal and diabetic groups. Short duration hyperglycemia has no role in producing inflammation and raising the levels of bioinflammatory marker C-reactive protein

Protein and plasma amino acid metabolism in cancer patients

Proteins present in the human body have numerous functions. Amino acids are the building blocks of proteins used for structural and regulatory functions of body. In cancer cachexia the patients develop anorexia, diminished intake of calories with significant abnormalities in carbohydrate, lipid and protein metabolism and progressive tissue depletion accompanied by weight loss. The gradual loss of host protein stores is central to this process which leads to amino acid imbalance in the blood and some times decreased levels of certain amino acids. Increased urinary amino acids occur in patients of certain cancers. These changes in the amino acid result into altered host defence against cancer and also the therapeutic complications in these patients. Extra nutritional support through gut and total parenteral nutrition [TPN] can alter the tumour host nutrition balance and repletion occur