Comparison of clinical effects between unipedicular and bipedicular approach to percutaneous vertebroplasty for osteoporosis vertebral compressive fractures / 实用医学杂志

Objective To compare the clinical effect and safety of percutaneous vertebroplasty between unipedicular and bipedicular approach for patients with osteoporosis vertebral compressive fractures. Methods A retrospective study was carried out on 147 patients with single osteoporotic vertebral compressive fractures from 2008 to 2014. Ninety-five cases were treated by PVP via a unipedicular approach (unipedicular group) and 52 cases were treated by the bipedicular approach (bipedicular group). The operation time, Cobb, frequency of X-ray exposure,complications like bone cement and postoperative VAS score were compared. Results No significant differences on the VAS score, cobb and complications were found (P > 0.05). However, there were significant differences on the operation time and X-ray exposure frequency (P<0.05). Conclusions No significant differences on clinical effects were found between the unipedicular and the bipedicular approach to percutaneous vertebroplasty for osteoporosis vertebral compressive fractures. However, the unipedicular approach can shorten the operation time and reduce X-ray exposure.

Percutaneous vertebroplasty in the treatment of vertebral hemangioma：bone cement operation / 中国组织工程研究

BACKGROUND:Increasing studies have found that, percutaneous vertebroplasty and bone cement are characterized by few traumas, few bleeding, simple operation, rapid recovery, rapid pain relief, and effective efficacy. However, tumor recurrence, symptom improvement, bone cement leakage and other complications make people controversial. OBJECTIVE: To explore and summarize clinical outcomes and safety of percutaneous vertebroplasty and bone cement for treating symptomatic vertebral hemangioma. METHODS:Fifty-two cases patients of symptomatic vertebral hemangioma (including 21 male and 31 female, aged 16-63 years) were treated with percutaneous vertebroplasty and bone cement. The lesions were found at T5-T12 in 36 cases and at L1-L3 RESULTS AND CONCLUSION: Al 52 patients were folowed up for at least 6 months after the operation. The Visual Analogue Scale and Oswestry Disability Index were significantly increased at 1 day, 3 weeks and 6 months post-surgery, when compared to before surgery (P< 0.05); the modified Macnab evaluation was excelent in 40 cases, good in 7 cases, fair in 3 cases, and poor in 2 cases, and the excelent and good rate was 90%. Only one patient had recurrence at 6 months after surgery and one patient had the leakage of bone cement. No other complications were found. Percutaneous vertebroplasty and bone cement could effectively cure vertebral hemangioma and relieve the pain, which is a safe treatment. in 16 cases. Clinical outcomes such as Visual Analogue Scale, Oswestry Disability Index and modified Macnab criteria, as wel as complication before and after surgery were evaluated.

A comparative study of tumor-associated macrophages isolation in colorectal carcinoma by Ficoll-Hy-paque density gradient centrifugation and Percoll density gradient centrifugation / 实用医学杂志

Objective Percoll density gradient centrifugation and Ficoll-Hypaque density gradient cen-trifugation, which are frequently-used methods for separation of tumor-associated macrophages (TAMs) from solid carcinoma were compared, in order to find an effective way to separate TAMs from colorectal carcinoma (CRC). Furthermore, we studied the best adherence time of separating macrophage among mononuclear cells. Methods specimens were collected from CRC patients , after digesting into single cells , TAMs were separated from the same specimen by 100% Ficoll, 35% percoll and 25% combined with 65% percoll respectively. After these pre-liminary separation, the collected cells were purified a second time by adherence separation. The purity of TAMs were detected by immunofluorescence. Results TAMs purity from Ficoll-Hypaque density gradient centrifugation was 80.18%, statistically higher than that from Percoll density gradient centrifugations (54.33% and 10.93% re-spectively). Conclusion Compared to Percoll density gradient centrifugation, Ficoll-Hypaque density gradient centrifugation is a more effective and simple way to isolate TAMs from colorectal carcinoma , suggesting it can be wildly used in clinical and basic medical research. 2-4 hours is the best adherence time for isolating macrophage.

Expression of long noncoding RNA MALAT1 gene in human nasopharyngeal carcinoma cell lines and its biological significance / 南方医科大学学报

<p><b>OBJECTIVE</b>To explore the expression of MALAT 1 in nasopharyngeal carcinoma (NPC) and its biological function.</p><p><b>METHOD</b>Real-time PCR was used to detect the expression of MALAT1 in 5-8F, C666-1, CNE-1, CNE-2, HONE-1, 6-10B and NP69 cell lines. CNE-1 cells engineered with MALAT1 RNA interference (RNAi) and RNA activation (RNAa) techniques were examined for changes in cell proliferation, invasion and metastasis using CCK8 assay, colony formation assay, Transwell in vitro invasion assay and wound-healing assay ability.</p><p><b>RESULTS</b>MALAT1 was highly expressed in 5-8F cells with a high metastatic potential, and lowly expressed in normal nasopharyngeal epithelium cells. Overexpression of MALAT1 by RNAa suppressed the expression of E-cadherin, promoted the expression of vimentin and enhanced the proliferation, invasion, and metastasis of CNE-1 cells.</p><p><b>CONCLUSION</b>MALAT1 can enhance the proliferation, invasion, and metastasis of CNE-1 cells.</p>

Effect on avascular necrosis of the femoral head with vascular endothelial growth factor / 中国矫形外科杂志

[Objective]To explore a new method for the therapy of avaseular necrosis of the femoral head with vascular endothelial growth factor(VEGF).[Method]Thirty New Zealand white rabbits were chosen as experimental animals and avascularnecrosis of the femoral head model of 22 rabbits were established.Animals were egually divided into three groups:VEGF group,natural repair group,normal group.The medicament was injected to the femoral head by percutaneous injection in group A and normal saline in group B.The repairing process of bone necrosis of femoral head were studied.[Result]Compared to the untreated model group(group C),the femoral head of VEGF groups showed significant new vessel formation,number of empty bone lacunae decreased and hematopoietic tissue improving in marrow cavity.X-ray film and CT revealed evident that veGF could enhance repairing of the femoral head.[Conclusion]Pereutaneous injection of VEGF to the necro femoral head could significantly enhance bone tissue angiogenesis and ameliorated repair of osteonecrosis,thus providing a potential method for the treatment of the femoral head necrosis.

Change of TNF-?and VEGF on glucocorticoid-induced avascular necrosis of femoral head in rabbits / 中国矫形外科杂志

[Objective]To establish animal model of avascular necrosis of the femoral head in rabbits induced by glucocorti-coid, to further investigate the pathogenesis of glucocorticoid-induced osteonecrosis.[Method]Twenty NewZealand white rabbitswere used and randomly divided into two groups.Group A was injected with horse serum and prednisone. Group B was used asthe control.The concentration of TNF-?was measured by ELISA in rabbits serum of group A and group B.Femoral head speci-mens were studied by histopathologic examination and the expression of VEGF by immunohistochemical examination.[Result]The concentration of TNF-?in rabbits serum of group A was higher than that of group B(P

CT and MRI Diagnosis of Pineal Region Germinoma / 实用放射学杂志

Objective To investigate the role of CT and MRI in diagnosis of the pineal region germinoma.Methods 11 cases of the pineal region germinoma proved by operation and pathology. CT and MRI features were analysed retrospectively. Results In 11 cases on CT most tumors were hyperattenuation or isoattenuation. Enhancement in most of the tumors were homogeneous. The characteristic MRI appearances of the pineal region germinoma were as follows: (1) On T 1WI, the lesions were isointense or slightly hypointense while isointense or slightly hyperintense on T 2WI. The pineal region germinoma showed no edema. (2) The lesions showed homogeneous or inhomogeneous Gd-DTPA enhancement in most of the tumors.Conclusion CT and MRI of multiplanar imaging and Gd-DTPA enhancement are helpful in diagnosis and differential diagnosis of pineal region germinoma. The tumors have sex, location and imaging characteristics.