RESUMO
OBJECTIVE: To determine whether inclusion of vitamin E into kidney storage solutions protects metabolism and tubular ultrastructure of stored rat kidney. METHODS: Rat kidneys were flush stored in Marshall's Citrate (MC) and MC + vitamin E (25% of LD 50 and 50% of LD 50) for 24 hours at 0 degrees C. After storage kidney slices were tested for gluconeogenesis and lactate dehydrogenase (LDH) activity, and examined for cellular ultrastructure. RESULTS: Kidneys stored in MC + vitamin E gave higher gluconeogenesis than those stored in MC alone (p < 0.001). Tubular ultrastructure was better preserved in the presence of Vitamin E. CONCLUSIONS: Vitamin E appears to protect the metabolism and ultrastructure of stored rat kidneys.
Assuntos
Animais , Gluconeogênese/efeitos dos fármacos , Rim/metabolismo , Preservação de Órgãos , Ratos , Ratos Sprague-Dawley , Vitamina E/farmacologiaRESUMO
The buccal smear test was used to screen for Turner syndrome in a sample of girls with severe short stature who did not have any other clinical features of that condition. The majority of the girls did not show X chromatin bodies in the buccal mucosal cells. None of those who qualified for chromosomal analysis showed an XO chromosomal pattern either. We conclude that reliability of the buccal smear test as a screening method for Turner syndrome remains doubtful.
Assuntos
Bochecha , Criança , Pré-Escolar , Citodiagnóstico , Feminino , Transtornos do Crescimento/etiologia , Humanos , Mucosa Bucal/química , Cromatina Sexual , Síndrome de Turner/complicaçõesRESUMO
Growth hormone deficiency is a recognised cause of severe short stature. A community of 16,001 Sri Lankan children aged between 5 and 6 years was screened for short stature using a simple growth chart, and a sample of 172 identified as short was investigated for the aetiology. Growth hormone deficiency was confirmed in 12 out of the 172 children using the insulin-induced hypoglycaemia test. Another group of 27 out of the 172 children who could potentially benefit from growth hormone therapy were identified using an exercise screening test. Growth hormone deficiency has not been previously documented in Sri Lanka.