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1.
China Journal of Chinese Materia Medica ; (24): 1564-1572, 2019.
Artigo em Chinês | WPRIM | ID: wpr-774520

RESUMO

To establish an HPLC characteristic fingerprint method of Fuke Qianjin Capsules,and determine the contents of its main components. The analysis was carried out on a Kromasil 100-5-C18 analytical column(4. 6 mm ×250 mm,5 μm) with gradient elution by acetonitrile(A)-0. 1% phosphoric acid aqueous solution(B),a flow rate at 1. 0 m L·min-1 and the detection wavelength of 254 nm.The column temperature was 30 ℃,and the injection volume was 10 μL. The determination method of genistin,jatrorrhizine,andrographolide and 14-deoxy-11,12-didehydroandrographolide index components were studied methodologically. The common mode of the characteristic fingerprint of Fuke Qianjin Capsules was set up with 8 common peaks,which were identified as genistin,jatrorrhizine,palmatine,berberine,andrographolide,14-deoxy-11,12-didehydroandrographolide,Z-ligustilide,and Z-3-butylidenephthalide,respectively,in comparison with the references. The similarities of 20 batches of Fuke Qianjin Capsules samples were above 0. 95. All of the above-mentioned 4 analytes could be well separated under the optimized chromatographic conditions. RSD of precision and repeatability experiment were both less than 1. 5%,and the sample solution was stable during 72 h. All of the compounds had a good linearity and linear range. The contents of genistin,jatrorrhizine,andrographolide,and 14-deoxy-11,12-didehydroandrographolide in 20 batches of Fuke Qianjin Capsules samples were 28. 66-56. 04,94. 77-197. 92,1 705. 33-4 148. 93 and 462. 16-1 225. 96 μg in each capsule,respectively. The developed HPLC characteristic fingerprint and quantitative analysis methods were reliable,accurate and sensitive,and could be used effectively evaluate the quality of Fuke Qianjin Capsules samples.


Assuntos
Cápsulas , Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas , Química , Compostos Fitoquímicos
2.
Chinese Traditional Patent Medicine ; (12)1992.
Artigo em Chinês | WPRIM | ID: wpr-681478

RESUMO

Objective: To determine the content of 14 deoxy 11,12 didehydroandrographolide in XIAOYANG LIDAN TABLETS by TLC Scanning.Methods: 14 deoxy 11,12 didehydroandrographolide was determined by dual wavelength TLC Scanning.Results: The calibration curve was linear within the range of 0.982~4.910?g of 14 deoxy 11,12 didehydroandrographolide. The average recovery and RSD were 99.4% and 2.14% ( n =5), respectively.Conclusion: The method is simple, accurate and reproducable.

3.
Chinese Traditional Patent Medicine ; (12)1992.
Artigo em Chinês | WPRIM | ID: wpr-682265

RESUMO

Objective: To study the changes of andrographolides in the drying process of the extract of Andrographis paniculata Nees. Methods: HPLC method was applied to analyze andrographolide and 14 deoxy 11, 12 didehydroandrographolide in the process. Results: The content of andrographolide descended rapidly in the whole drying process, while the content of 14 deoxy 11, 12 didehydroandrographolide ascended at first 12 hours, declined in content was slowly to follow. Conclusion: Baking temperature is not the only main factor to stimulate the transformation of andrographolide and 14 deoxy 11, 12 didehydroandrographolide.

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