RESUMO
ABSTRACT: The objective of the present research was to develop a protocol for micropropagation of Anthurium bonplandii and Anthurium maricense by direct organogenesis. Nodal segments, with two or three nodes, were used as explants. The cultures were kept in a growth chamber at a temperature of 25±2ºC, under a photoperiod of 16 hours and a luminosity of 30μmol m-2 s-1. At 60 days, the number of regenerated buds per explant (NBE) was counted. The experiment was carried out in an entirely randomised design consisting of six treatments for six different concentrations of 6-benzylaminopurine (6-BA) added to the P2 (Pierik) medium (0.0, 1.11, 2.22, 3.33, 4.44, and 5.55µM). All the treatments were performed in four repetitions with 10 culture tubes containing one explant each. The regression analyses were adjusted to a quadratic model, with R2 = 88.7% and 62.4% for A. maricense and A. bonplandii, respectively. The regressions indicate that the addition of 6-BA to the P2 medium resulted in larger values of NBE in both the species. The ideal 6-BA concentration for micropropagation varied depending on the species, with 2.5 and 1.7 NBE determined at 6-BA concentrations of 4.70 and 3.37µM for A. maricense and A. bonplandii, respectively.
RESUMO: O objetivo do trabalho foi desenvolver um protocolo para micropropagação de Anthurium bonplandii e A. maricense por meio da organogênese direta. Foram utilizados como explantes, segmentos nodais contendo de dois a três nós. As culturas foram mantidas em sala de crescimento com temperatura de 25±2ºC, fotoperíodo de 16 horas e intensidade luminosa de 30μmol m-2 s-1. Aos 60 dias, avaliou-se o número de brotações regeneradas por explante (NBE). O experimento foi em um delineamento inteiramente casualizado composto por seis tratamentos referentes ao meio P2 (Pierik) adicionado de seis concentrações de 6-benzilaminopurina (6-BA): 0,0; 1,11; 2,22; 3,33; 4,44 e 5,55µM, com quatro repetições de dez tubos de ensaio, contendo um explante cada. As análises de regressão se ajustaram ao modelo quadrático com R2=88,7% e 62,4% para A. maricense e A. bonplandii, respectivamente. As regressões indicaram que adições de 6-BA ao meio P2, promoveram maior número de NBE em ambas as espécies. A concentração de 6-BA estimada como ideal para a micropropagação varia de acordo com a espécie, sendo estimado 2,5 e 1,7 NBE, quando a concentração de 6-BA for igual a 4,70 e 3,37µM para as espécies de A. maricense e A. bonplandii, respectivamente.
RESUMO
In order to explore appropriate measures to promote germination after the harvest of Epimedium pseudowushanense, 6-BA, urea, ammonium bicarbonate and GA₃ were chosen to spray on the root and rhizomes, and then the biological indicators such as branches, leaf length, leaf width, plant height and so on, were measured in different periods, and the contents of epimedin A, epimedin B, epimedin C and icariin in the dry leaves were detected by HPLC. Results showed that 6-BA 90 mg·L⁻¹(B1), 6-BA 60 mg·L⁻¹(B2),6-BA 30 mg·L⁻¹+urea 300 mg·L⁻¹ (C1), 6-BA 60 mg·L⁻¹+urea 300 mg·L⁻¹(C2),6-BA 60 mg·L⁻¹+ ammonium bicarbonate 300 mg·L⁻¹(C4) significantly increased bud germination in the early period, and the plants quickly set up new system of photosynthesis, the branches in a month of which were higher than the control group respectively by 165.9%, 115.76%, 103.86%, 104.50%, 81.67%.However the branches developed the next year and the dry weight of leaves per plant in group B1 and B2 were much lower than that in control group. The groups that use 6-BA and nitrogen at the same time reaped a good yield of leaves even though the treatment had no significant influence on the branches developed the next year. The dry weight of leaves of C1, C2, C4 treatments were 36.80%, 32.84%, 45.97% more than the control group respectively. Therefore, C1, C2 and C4 treatments are the more appropriate to promote recovery after harvest. Furthermore, different groups, except 10 mg·L⁻¹ 6-BA treatment significantly reduced the content of epimedin C, other groups didn't have any significant effect on the contents of such flavonoids.
RESUMO
Objective: The roots of Paris polyphylla var. chinensis were used as explants to establish a rapid propagation technique. Methods: The effects of hormone 6-BA, 2,4-D, NAA, IBA, KT, IAA, ceftriaxone, activated carbon, etc on callus induction, differentiation of adventitious bud, and formation of adventitious roots were studied to find out the best culture medium formulation by using MS as basic culture medium. Results: The best medium for callus induction of P. polyphylla var. chinensis was MS + 6-BA 1.0 mg/L + NAA 1.5 mg/L + 2,4-D 3.0 mg/L, and the best medium for differentiation of adventitious bud was MS + 6-BA 3.0 mg/L + IAA 0.3 mg/L + KT 1.0 mg/L + ceftriaxone 300 mg/L, while the best medium for formation of adventitious roots was 1/2 MS + IBA 0.5 mg/L + NAA 0.1 mg/L + activated carbon 0.5%. The tissue cultured seedling was moved to sterile water for 5 d when the first leaf emerged and four adventitious roots appeared, then the seedling was transplanted into the soft soil. The tissue cultured seedling would grow strongly with 100% survival rate. Conclusion: The adventitious bud is inducted and then the root from bare-root seedling is cultured on the basis of obtained callus. Finally the best conditions to foster the tissue cultured seedling of P. polyphylla var. chinensis have been screened.