Research progress of anti-tumor effect and mechanism of CDDO and its derivatives / 中国药房

2-cyano-3，12-dioxooleana-1，9 （11）-dien-28-oic acid （CDDO） is a compound synthesized by taking oleanolic acid， a natural triterpene， as a precursor or precursor， and transforming three modifiable functional groups in the molecule through a series of chemical structure modification. In order to improve its anti-tumor activity， CDDO derivatives are further synthesized. In this paper， the research results of anti-tumor effects and mechanisms of CDDO and its derivatives in recent years are summarized. It is found that CDDO and its derivatives have a wide range of anti-tumor effects， and can show significant anti-tumor effects on breast cancer， pancreatic cancer， lung cancer and ovarian cancer at low concentrations such as micromole or even nanomole， among which CDDO methyl ester compound （CDDO-Me） and CDDO imidazolidinone compound （CDDO-Im） have the most obvious effects. CDDO and its derivatives exert anti-tumor activity mainly by inducing tumor cell apoptosis， and regulating metabolic reprogramming and immune microenvironment. The involved pathways mainly include Janus protein tyrosine kinase （JAK）/ signal transduction and transcription activation protein 3（STAT3） signal pathway， nuclear factor E2-related factor 2 （NRF2） signal pathway， phosphatidylinositol 3 kinase （PI3K）/protein kinase B （also known as Akt）/mammalian rapamycin target protein （mTOR） signal pathway， Wnt/β-catenin signal pathway， nuclear factor κB signal pathway.

Chemical constituents from n-butanol parts of Lycii Cortex / 中草药

Objective: To study the chemical constituents from n-butanol-souluble part of Lycii Cortex (the root bark of Lycium chinense). Methods: The air-dried Lycii Cortex were powdered and extracted with 70% ethanol under reflux. After the removal of solvent under reduced pressure, the crude extract was extracted with petroleum ether, ethyl acetate and n-butanol successively. The compounds were isolated and purified by silica gel, Sephadex LH-20, ODS and semi-prepared high performance liquid chromatography from the n-butanol part of Lycii Cortex. The structures were identified by nuclear magnetic spectrometry, mass spectrometry and other spectral analyses. Results: Ten compounds were isolated from n-butanol parts of Lycii Cortex and characterized as (1'S,2R,5S,10R)-2-(1',2'-dihydroxy-1'-methylethyl)-6,10-dimethylspiro [4,5] dec-6-en-8-one 2'-O-β-D- glucopyranoside (1), (1'R,2R,5S,10R)-2-(1',2'-dihydroxy-1'-methylethyl)-6,10-dimethylspiro [4,5] dec-6-en-8-one 2'-O-β-D- glucopyranoside (2), (1R,6R,9S)-6,9,11-trihydroxy-4,7-megastigmadien-3-one 11-O-β-D-glucopyranoside (3), vanillic acid-4-O-β- D-glucopyranoside (4), 3,4-dihydroxyphenylpropionic acid (5), 3,4-dihydroxybenzenepropionic acid methyl ester (6), glucosyringic acid (7), dihydrophaseic acid 3'-O-β-D-glucopyranoside (8), isoscoploletin-β-D-glucoside (9) and fabiatrin (10). Conclusion: Compound 3 is isolated from Solanaceae family for the first time and compounds 1, 2 and 4 are isolated from Lycium genus for the first time. The NMR data of compound 2 is first reported as well.

Triterpenoids isolated from leaves of Ledum palustre / 中草药

Objective: To study the chemical constituents from the leaves of Ledum palustre and their antitumor activities. Methods: The chemical constituents were isolated and purified by chromatography of silica gel column, recrystallization and HPLC, and their structures were elucidated by spectral analysis. The in vitro cytotoxic activities of the isolated compounds were studied by MTT method. Results: Nine compounds were isolated and identified as ledumone (1), uvaol (2), lepenone (3), α-amyrenone (4), ursolic acid (5), lupeol (6), α-amyrin (7), fern-9(11)-ene-2α,3β-diol (8), and fernenol (9). Conclusion: Compound 1 is new named as ledumone, and compounds 4, 8, and 9 are isolated from Ledum palustre for the first time. Fernane type triterpene 1, 8, and 9 show no significant cytotoxicities against A549 and K562 cells.

Steroidal components from rhizome of Smilacina henryi / 中草药

Objective To study the chemical components from the roots of Smilacina henryi. Methods Silica gel, Sephadex LH-20, and preparation of thin layer chromatography techniques were used for isolation, and 1D, 2D-NMR, IR, and HR-ESI-MS data were used for structure identification. Results Seven steroidal components were obtained and its chemical structures were elucidated as (25S)-5α-spirostan-9(11)-en-3β,17α-diol 3-O-β-D-glucopyranosyl-(1→2)-β-D-galactopyranoside (1), (25S)-5α-spirostan-9(11)-en- 3β,17α-diol (2), (25S)-5α-spirostan-9(11)-en-3β,17α-diol 3-O-β-D-glucopyranoside (3), diosgenin (4), aspidistrin (5), henryioside A (6), and henryioside B (7). Conclusion Compound 1 is a new steroidal saponin named henryioside D, and compounds 2-5 are isolated from the plant for the first time.

Your dilemma, my identity: Unusual immunogenetic profiles of pediatric B cell acute lymphoblastic leukemia.

B-cell acute lymphoblastic leukemia (B-ALL) is characterized by CD19 expression, which is one of the most important prerequisites, along with expression of CD10, CD22 and/or CD79a. Rearrangements involving MLL gene are seen in CD10− B-ALL (pro-B cell origin) and t(9;11)(p21;q23) is most commonly reported in acute myeloid leukemia (AML), where it is known to carry very good prognosis in pediatric AMLs and rarely in acute lymphoblastic leukemia (ALL). We report a case of CD10+, CD19− pediatric ALL with rearrangements of MLL gene as a result of t(9;11)(p21;q23), thus conferring a very poor prognosis. The case emphasizes use of comprehensive panel of antibodies for fl ow cytometric immunophenotyping and cytogenetic correlation for correct diagnosis and prognostication. KEY WORDS: Acute lymphoblastic leukemia, CD19, MLL gene, t(9;11)(p21;q23)

Chemical constituents of whole plant of Euphorbia thymifolia / 中草药

Objective: To separate and identify the chemical constituents from the whole plant of Euphorbia thymifolia. Methods: The constituents were isolated and purified by various chromatographic methods and were identified on the basis of spectral analysis and physicochemical characters. Results: Thirteen compounds were separated and identified as luteolin (1), apigenin (2), quercetin (3), kaempferol (4), ethyl galloyl acid (5), p-coumaric acid (6), protocatechuic acid (7), gallic acid (8), caffeic acid (9), 3, 4-seco-8βH-ferna-4 (23), 9 (11)-dien-3-oic acid (10), 3, 4-seco-oleana-4 (23), 18(19)-dien-3-oic acid (11), tricosanol (12), and β-sitosterol (13), respectively. Conclusion: Addition to the compound 5, the other compounds are isolated from this plant for the first time.

Chemical constituents of Euphorbia royleana / 中草药

Objective: To study the chemical constituents in the aerial parts of Euphorbia royleana. Methods: The constituents were isolated and purified by chromatographic of silica gel, Sephadex LH-20, RP18, and MCI columns, and their structures were elucidated by spectroscopic analyses. Results: Twelve compounds were isolated from the 70% acetone extracts in the aerial parts of E. royleana and their structures were identified as (6S, 9R)-roseoside (1), 13-carboxyblumenol C 9-O-β-glucoside (2), 3, 3'-dimethylellagic acid-4-O-β-D-glucopyranoside (3), cycloart-23-ene-3β, 25-diol (4), 23(E)-25-methoxycycloart-23-en-3β-ol (5), α-amyrin (6), triptohypol F (7), 9(11), 12-dieneoleana-3β-ol (8), friedelane-3β, 29-diol (9), D:A-friedoolean-29-ol-3-one (10), dischidiol (11), and lupeol (12). Conclusion: Compounds 1, 2, 7-11 are obtained from the plants in Euphorbia L. for the first time and compounds 3-5 and 12 are isolated from this plant for the first time.

Chemical constituents of Ramaria flava / 中国药学杂志

OBJECTIVE: To study the chemical constituents of Ramaria flava. METHODS: Several chromatographic methods were used to isolate the chemical constituents from this mushroom. Their structures were elucidated on the basis of spectral data. RESULTS: Eleven compounds were identified as: adenine (I), 5α, 8α-epidioxyergosta-6, 22-diene-3β-ol (II), 5α, 8α-epidioxyergos-ta-6, 9(11), 22-triene-3β-ol (III), Phenylacetic acid (IV), Bis (2-ethylhexyl) phthalate (V), 5α, 8α-epidioxyergosta-6, 9 (11)-diene-3β-ol (VI), stigmasterol (VII), D-allitol (VIE), α-D-(+) Glucose (IX), 3-O-β-Z)-Glucopyranosyl-22E, 24R-5α, 8α-epidioxy-ergosta-6, 22-diene (X), and 3-O-β-D-Glucopyranosyl-22E, 24R-5α, 8α-epidioxyergosta-6, 9, 22-triene(XI). CONCLUSION: All the 11 compounds were isolated from Ramaria flava for the first time. Copyright 2012 by the Chinese Pharmaceutical Association.

Changes in Perceptions of George W. Bush's Personality in the Wake of the September 11 2001 World Trade Center Attacks / Cambios en la percepción de la personalidad de George W. Bush posterior a los ataques al World Trade Center el 11 de Septiembre de 2001

Using data gathered just before and just after the September 11th terrorist attacks, we examine how perceptions of Bush's personality changed in the following two weeks. Fifty participants provided ratings of Bush using the California Q-sort at various times before (including immediately before) and after the attacks. At each time interjudge agreement was strong. There was general consistency between the pre-and post-attack assessments, but the common view of Bush shifted in several important ways. Consistent with his soaring popularity, the changes were toward more positive perceptions, even for characteristics unrelated to the attacks. Findings are discussed in terms of possible mechanisms driving the changes in perception. These findings, which are based on careful assessments conducted shortly before the emergence of any hint of what was to come, provide a unique perspective on changes in Bush's image as they unfolded in the immediate wake of the terrorist attacks.

Utilizando información recolectada justo antes y justo después de los ataques terroristas de septiembre 11 de 2001, se examinó cómo las percepciones de la personalidad de George Bush cambiaron en las dos semanas subsecuentes. Cincuenta participantes proveyeron puntajes sobre Bush utilizando el California Q-Sort en diferentes momentos antes (incluyendo el "inmediatamente antes") y después de los ataques. En cada momento temporal el acuerdo entre-jueces fue alto. Hubo consistencia general entre las evaluaciones antes y después de los ataques, pero la manera de ver a Bush tuvo cambios importantes en algunos puntos específicos. De manera consistente con su altísima popularidad, los cambios fueron hacia percepciones más positivas, aún en características no relacionadas con los ataques. Los hallazgos son discutidos en términos de mecanismos positivos que conducen los cambios en la percepción. Estos hallazgos, que están basados en evaluaciones cuidadosamente realizadas antes de siquiera haber tenido noción de lo que iba a suceder, proveen una perspectiva única de los cambios en la imagen de Bush tal y como se desarrollaron después de los ataques terroristas.