RESUMO
To study the effect of Blattidae compound PB on chronic UC induced by alloantigen combined with acetic acid in rats, and to preliminarily explore its mechanism. Methods Chronic UC model was established using alloantigen combined with acetic acid. Normal group and model group were given sodium chloride solution by enema, while the other groups were given respectively to the SASP, the Changyanning and PB low, medium and high doses. The experiment was determined by evaluating DAI, CMDI, HS and the visceral index. Serum IL-4, IL-8, IL-17, CRP, IgG and colonic mucosa TNF-MPO EGF were detected by ELISA. Results Compared with normal group, the levels of IL-8, IL-17, INF-7, CRP, IgG, TNF-a, MPO in model group significantly increased, while the levels of 1L-4, EGF decreased significantly (P <0. 01, P <0. 05). But the levels of DAI,CMDI, HS, IL-8, IL-17, INF-7, CRP, IgG, TNF-ot, MPO in each dose of PB groups were significantly lower than those of model group, and the levels of IL-4, EGF were significantly higher than those of model group (P <0. 01, P < 0. 05). Conclusions Blattidae compound PB can effectively relieve chronic ulcerative colitis induced by alloantigen combined with acetic acid in rats, and its mechanism may be related to promoting Th2 cell activation, changing Thl-prone Thl/Th2 balance to Th2, which regulated cell immunity and humoral immunity to achieve the steady state immune balance.
RESUMO
BACKGROUND: In the Korean population, gene frequencies of Human Neutrophil Alloantigen-1 (HNA-1), HNA-2, HNA-4, and HNA-5 were determined using serological and genotyping methods. However, no study assessing the gene frequencies of HNA-3 among the Korean population has been conducted. The aims of this study were to report the gene frequencies of HNA-3, to estimate the risk of HNA-3a (or HNA-3b) alloimmunization, and to secure donors of granulocytes for anti-HNA-3 antibodies among the Korean population. METHODS: Genotyping of HNA-3a and HNA-3b genes of 110 healthy and unrelated Korean donors was performed using a polymerase chain reaction with sequence-specific primers. RESULTS: The observed frequencies of HNA-3a and HNA-3b were 0.695 and 0.305, respectively, among the Korean population. The HNA-3a gene frequency of Koreans was significantly lower than those of American Whites and Germans (P<0.05). The risk of HNA-3a and HNA-3b alloimmunization due to pregnancy (transfusion) was 0.065 (0.084) and 0.147 (0.250), respectively, among the Korean population. The risk of HNA-3a alloimmunization was significantly higher in the Korean population than in the German and American White populations (P<0.05). CONCLUSION: The gene frequencies of HNA-3 and the risk of HNA-3a (HNA-3b) alloimmunization due to pregnancy or transfusion among the Korean population were determined. We also identified individuals who were HNA-3a/3a or HNA-3b/3b for the granulocyte panel which could be used for anti-HNA antibody identification.