Chemical constituents from stems and leaves of semi-mangrove plant, Barringtonia racemosa / 中草药

Objective: To study the chemical constituents of the stems and leaves of semi-mangrove plant, Barringtonia racemosa. Methods: The chemical constituents of B. racemosa were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies and preparative HPLC. Their structures were identified by physicochemical properties, spectroscopic analysis, as well as comparisons with the data reported in literature. Results: A total of 17 compounds were isolated from the 90% ethanol extract of the stems and leaves of B. racemosa, which were identified as chrysin (1), ayanin (2), genkwanin (3), rhamnocitrin (4), tricin (5), dillenetin (6), 5,3'-dihydroxy-7,4'-dimethoxyflavone (7), 5,7,3',4'-tetramethoxyflavone (8), 5-hydroxy-6,7,8,3',4'- pentamethoxy flavone (9), petasitolone (10), sarmentol F (11), dehydrovomifoliol (12), blumenol A (13), 10-hydroxyaristolan-9-one (14), alphitolic acid (15), oleanolic lactone (16), and 11,12-dehydroursolic acid lactone (17). Conclusion: All compounds are isolated from the genus Barringtonia for the first time.

Chemical constituents from stems of Syzygium jambos var. jambos and their in vitro cytotoxicity / 中草药

Objective: To study the chemical constituents from the stems of Syzygium jambos var. jambos. Methods: The phyotchemicals were isolated by using various chromatographic techniques such as silica gel, Sephadex LH-20, alumina column chromatography, and preparative HPLC process. The structures were elucidated on the basis of spectral data analysis. Their cytotoxicity against human liver cancer cells Huh-7 was conducted using MTT assay. Results: Twelve compounds were isolated from the EtOAc and MeOH extracts in the stems of S. jambos and identified as alphitolic acid (1), urolithin A (2), dibutyl phthalate (3), betulinic acid (4), ursolic acid (5), 1-(4-methoxyphenyl)-1, 2-propanediol (6), 2, 6-dimethoxy-1, 4-benzoquinone (7), diisobutyl phthalate (8), β-sitosterol (9), 3-acetyl-ursolic acid (10), asiatic acid (11), and arjunolic acid (12), respectively. All tested compounds 1, 7, 10, and 11 + 12 mixture did not show the cytotoxic activities towards human tumor cells Huh-7 in the test range up to 50 μmol/L. Conclusion: Except compound 4, the other 11 compounds are obtained from this plant for the first time. All tested compounds exhibit no significant cytotoxic activity against Huh-7 cells.

An HPLC-UV and HPLC-ESI-MS based method for identification of anti-inflammatory triterpenoids from the extracts of Ugni molinae / Método CLAE-UV y CLAE-IES-MS para la identificación de triterpenoides anti-inflamatorios de los extractos de Ugni molinae

The aim of this study was to establish an analytical method to detect the presence of the responsible triterpenoids of the anti-inflammatory activity of the leaves of Ugni molinae (murtilla). Successive leaves extracts of EtOAc (EAE) and ethanol (TEE) were prepared, obtaining for the first time from TEE a triterpenoid-rich sub-fraction (TF). The topical anti-inflammatory activity of TF was assessed (43.3 percent at 1 mg/ear) by means of the TPA-induced mouse ear oedema model, which was compared to EAE (83.1 +/- 3.2 percent) and TEE (78.3 +/- 11.8 percent) activities, both previously evaluated by us. These extracts were characterized in their triterpenoids by HPLC-UV and HPLC-ESI-MS. We demonstrated that TF has triterpenoids responsible in part of the anti-inflammatory activity, among them, madecassic and maslinic acids. These two compounds have been reported for the first time for this species. ED50 for madecassic and alphitolic acids are also here reported.

El objetivo de este trabajo fue establecer un método analítico para determinar la presencia de los triterpenoides responsables de la actividad anti-inflamatoria de las hojas de Ugni molinae (murtilla). Fueron preparados los extractos seriados de EtOAc (EAE) y etanólico (TEE) desde sus hojas, obteniendo desde el TEE por primera vez una sub-fracción rica en triterpenoides (TF). Se demostró la actividad anti-inflamatoria tópica del TF por el modelo de edema de oreja de ratón inducida por TPA (43,3 por ciento a 1 mg/oreja), la cual fue comparada con las de los EAE (83,1 +/- 3,2 por ciento) y TEE (78,3 +/- 11,8 por ciento) determinadas en nuestros estudios previos. Dichos extractos fueron caracterizados en sus triterpenoides por CLAE-UV y CLAE-IES-MS. Demostramos que el TF contiene triterpenoides responsables en parte de la actividad anti-inflamatoria, entre ellos, los ácidos madecásico y maslínico, reportados por primera vez para esta especie. Se informan además las DE50 para los ácidos madecásico y alfitólico.

A novel lupane triterpene from seeds of Ziziphus jujuba var.spinosa / 中草药

Objective To study the chemical constituents from the seeds of Ziziphus jujuba var.spinosa,which is used as a sedative herbal medicine in China.Methods The constituents were separated and purified by silica gel column.Their structures were elucidated based on their physicochemical properties and spectral analysis.Results Six compounds were obtained from 95% EtOH extracts in the seeds of Z.jujuba var.spinosa.They were identified as 2?,3?-dihydroxy-lup-20(29)-en-28-oic acid methyl ester(Ⅰ),?-sitosterol(Ⅱ),betulin(Ⅲ),betulinic acid(Ⅳ),hexadexanoic acid 2,3-dihydroxypropyl ester(Ⅴ),and daucosterol(Ⅵ).Conclusion Compound Ⅰ is a novel lupane triterpene named as alphitolic acid methyl ester,compound Ⅴ is obtained from the seeds of Z.jujuba var.spinosa for the first time.