RESUMO
The Brazilian Amazon Region is a highly endemic area for hepatitis B virus (HBV). However, little is known regarding the genetic variability of the strains circulating in this geographical region. Here, we describe the first full-length genomes of HBV isolated in the Brazilian Amazon Region; these genomes are also the first complete HBV subgenotype D3 genomes reported for Brazil. The genomes of the five Brazilian isolates were all 3,182 base pairs in length and the isolates were classified as belonging to subgenotype D3, subtypes ayw2 (n = 3) and ayw3 (n = 2). Phylogenetic analysis suggested that the Brazilian sequences are not likely to be closely related to European D3 sequences. Such results will contribute to further epidemiological and evolutionary studies of HBV.
Assuntos
Humanos , Carcinoma Hepatocelular , Movimento Celular/fisiologia , Neoplasias Hepáticas , Metaloproteinase 9 da Matriz/genética , Transdução de Sinais/fisiologia , Inibidor Tecidual de Metaloproteinase-1/genética , Linhagem Celular Tumoral/citologia , Linhagem Celular Tumoral/fisiologia , Colagenases/genética , Dipeptídeos/farmacologia , Inibidores de Metaloproteinases de Matriz , Metaloproteinase 1 da Matriz/genética , /genética , /genética , /genética , Inibidores de Proteases/farmacologia , Transdução de Sinais/efeitos dos fármacos , TransfecçãoRESUMO
An alpha actin gene segment, isolated from Nile tilapia (Oreochromis niloticus), was characterized by nucleotide sequencing, predicted amino acid sequence and Southern blot hybridization. Genomic DNA amplification resulted in a 1063-bp fragment corresponding to a partial alpha-cardiac muscle actin gene containing exons 3 to 6. Southern blot analysis of the restriction-digested DNA revealed that the Nile tilapia genome contains multiple muscle actin isoforms. Although comparison of the nucleotide sequence, amino acid residues and exon-intron organization of the isolated actin gene with those of other vertebrates showed a high level of identity, diagnostic amino acid residues can still be correlated to distinct actin genes in fish species.