RESUMO
Objective To establish a method for Emodin assay in Ampelopsis radix, and to improve the quality control of Ampelopsis radix. Methods Reversed phase high performance liquid chromatography was used. The column: Apollo-C18 (4.6 mm×250 mm, 5 μm); mobile phase: methanol:0.2% phosphoric acid (85:15); flow rate: 1.0 ml/min; column temperature: room temperature; detection wavelength: 220 nm. Results With the specified chromatographic conditions, there was a good linear relationship for Emodin in Ampelopsis radix in the range of 0.124-3.968 μg/ml. The linear regression equation was Y= 53 962X–966.46, r=0.999 7. The average recovery was 99.7%. RSD=2.5% (n=9). Conclusion This method is accurate, sensitive and repeatable. It is suitable for the determination of emodin in Ampelopsis radix.
RESUMO
Objective: To analyze the effect of starch composition on the dissolution of small molecular alkaloids in Aconiti Radix when co-decocted with medicines rich in starch, and to explore the mechanism of the effect of starch on alkaloids. Methods: RRLC-Q- TOF-MS was used to analyze the effect of starch on the dissolution of alkaloids in the decoctions. HPLC-UV was applied to determine the content of alkaloids in the decoctions of starches with alkaloids, and analysis of variance and partial least square method were used to analyse the difference. Results: The dissolution of alkaloids in Aconiti Radix, including the diester-, monoester-, and amine- diterpenoid alkaloids, wsa obviously decreased when co-decocted with starch, especially the monoester- and amine-diterpenoid alkaloids co-decocted with Pinelliae Rhizoma, Ampelopsis Radix, starch of Pinelliae Rhizoma, starch of Ampelopsis Radix, and normally sold starch, while the content of diester-diterpenoid alkaloids was increased when co-decocted with starch-removed Pinelliae Rhizoma and Ampelopsis Radix. When co-decocted directly with starch, free alkaloids in water decoction was decreased compared with the decoction without starch. Conclusion: Starch could inhibit the dissolution of alkaloids in Aconiti Radix in the decoction. Free Aconitum alkaloids could be integrated by the starch, which could finally affect their content in decoction.
RESUMO
Objective: Under the guidance of eighteen antagonisms of Chinese materia medica, to analyze the effect of Aconiti Radix Cocta combined with Ampelopsis Radix in different ratios on the characteristic constituents and to explore the in vitro compatibility mechanism of Aconiti Radix Cocta and Ampelopsis Radix. Methods: A certain weight of Aconiti Radix Cocta was combined with various amounts of Ampelopsis Radix, and a rapid resolution liquid chromatography coupled with quadruple-time-of-flight mass spectrometry (RRLC-Q-TOF-MS) method based on chemical profiling approach was used to determine the content of alkaloids in decoctions and sediments. The aconitine alkaloids were established and were subjected to PLS-DA and ANOVA to provide intergroup difference and distribution characteristics. In addition, the method of UV spectrophotometer was adopted to determine the content of starch and tannins in Ampelopsis Radix. Results: There were certain differences among each group, especially between the single Aconiti Radix Cocta and Aconiti Radix Cocta combined with Ampelopsis Radix at the ratio of 1:3. With the increasing of the ratio of Ampelopsis Radix in the combination, the content of alkaloids appeared to increase in the decoction while decrease in the sediment. After determination, the mass fractions of starch and tannic acid in Ampelopsis Radix were 25.44% and 0.31%, respectively. Conclusion: The aconitine alkaloids could be deposited with the starch in Ampelopsis Radix, meanwhile decreased in the decoction, which establishes the foundation for the further research on the chemical mechanism of Aconiti Radix Cocta combined with Ampelopsis Radix.