RESUMO
Objective: To establish an ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of 23 plant growth regulator (PGR) residues in Chinese materia medica (CMM). Methods: Samples were extracted with acetontrile, and then determined by UPLC-MS/MS directly without further clean up. The matrix-matched external standard method was used for quantitative analysis. Results: The calibration curves showed good linearity in each range with correlation coefficients greater than 0.99. The limits of detection (LODs) were 0.01-20.80 ng/mL for the 23 PGRs spiked in Codonopsis pilosula and Angelica dahurica. The recoveries of the 23 PGRs spiked in C. pilosula and A. dahurica at three levels of 0.01, 0.04, and 0.1 mg/kg were in the range of 71.0%-101.4%, the relative standard deviation (RSDs) were 0.8%-15.2%. Commonly used CMM including eight species (63 batches) was analyzed by this method. Conclusion: The method proved to be simple, rapid, sensitive, accurate, and suitable for the simultaneous determination of 23 PGR residues in CMM
RESUMO
Objective: To study the effects of 10 kinds of furanocoumarines in Angelica dahurica (oxypeucedanin, isoimperatorin, phellopyerin, isoimperatorin, imperatorin, byakangelicol, angelicin, scopoletin, umbelliferone, and byakangelicine) on the intestinal absorption and transportation of vincristine. Methods: The apparent permeability coefficient (Papp) of vincristin was used as evaluated parameter, and Caco-2 (human colon cancer cell lines) cell monolayer was used as intestinal epithelial cell model to study the effects of 10 furanocoumarins on transportation of vincristine in Caco-2 cell monolayer. Results: There were different effects of the 10 furanocoumarins on transportation of vincristine. Oxypeucedanin, isoimperatorin, and imperatorin could improve the transport of vincristine in Caco-2 cell monolayer (P < 0.05), while isoimperatorin, byakangelicol, angelicin, umbelliferone, and byakangelicine could inhibit the transport of vincristine in Caco-2 cell monolayer (P < 0.01). However, phellopyerin and scopoletin have no effects on the transport of vincristine on Caco-2 cell. Conclusion: Different kinds of furanocoumarins have different effects on intestinal transport of vincristine, which may be related to the structure of furocoumarins and the functional group about their connection.