A new hexenol glycoside from Buddleja officinalis / 中国中药杂志

The chemical constituents of the flower buds of Buddleja officinalis were investigated in this study. Eight compounds were isolated from the water extract of B. officinalis by column chromatography, and their structures were elucidated on the basis of physicochemical properties and spectral data. These compounds were identified as(Z)-hex-3-en-1-ol-1-O-β-D-glucopyranosyl-(1→2)-[β-D-xylcopyranosyl-(1→6)]-β-D-glucopyranoside(1), ebracteatoside B(2), jasmonic acid-11-O-β-D-glucopyranoside(3), 6-hydroxyluteolin-7-O-β-D-glucopyranoside(4), luteolin-7-O-galacturonide(5), vicenin-2(6), decaffeoylverbascoside(7), and 6-O-(E)-feruloyl-D-glucopyranoside(8). Compound 1 is a new 3-hexenol glycoside. Compounds 2, 3, and 6 were isolated from Buddleja genus for the first time, and compounds 4 and 5 were isolated from this plant for the first time.

Buddleja asiatica Lour. Derived Phytochemicals against Staphylococcus aureus Causing Skin Diseases

Phytochemicals from Buddleja asiatica plant extract are traditionally used to cure skin diseases. It is caused by Staphylococcus aureus. Molecular docking method applied using “Biovia Discovery Studio”. “High positive values of -CDOCKER energy and -CDOCKER interaction energy” suggested that lignoceric acid can effectively deactivate the L-lactate dehydrogenase enzyme thereby interrupting the life cycle of the organism

Buddleja asiatica Derived Phytochemicals against Diarrhea

Plants produce nonnutritive compounds that are often extracted to make herbal medicines. It has been reported that extracts of Buddleja asiatica are effective to treat diarrhea. One of the causes ofDiarrhea is an infection by Campylobacter jejuni. The objective of the study is to identify the phytochemical of Buddleja asiatica capable of curing Diarrhea. ATP-phosphoribosyl transferase enzyme plays an important role in Purine Metabolism. Molecular docking method applied using “Biovia Discovery Studio”. “High positive values of -CDOCKER energy and -CDOCKER interaction energy” suggested that Lignoceric acid can effectively deactivate the transferase enzyme thereby interrupting the life cycle of the organism

Effect of Buddleja officinalis eye drops to inflammatory factors of lacrimal gland cells of castrated male rabbit with dry eye / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM:To observe the effect of different concentrations Buddleja offcinalis eye drops(BOED)to inflammatory factors of lacrimal gland cells of castrated male rabbit with dry eye.<p>METHODS:Forty-two healthy adult New Zealand rabbits were randomly divided into 7 groups, 6 rabbits each group. Group A: blank group; Group B: model group; Group C: low concentrations BOED group; Group D: medium concentrations BOED; Group E: high concentrations BOED; Group F: the placebo group; Group G: testosterone groups. All rabbits of groups B to G were cut off bilateral testis and epididymis except group A. The rabbits of groups C, D, E and F were dropped relevant eye drops except groups A and B. All rabbits were tested for Schimer I test(SIT)and break-up time(BUT)before operation and 4wk after operation. Expressions of inflammatory factors of TNF-α, IL-1β from lacrimal gland cells were checked in immunohistochemical staining method.<p>RESULTS:1)Comparison of SIT and BUT: Compared with the groups D, E and G, group C had statistical significance(<i>P</i><0.01). Compared with groups D and E, G groups, there was no significant difference among those group after operation(<i>P</i>>0.05); 2)Comparison of inflammatory factors of TNF-α, IL-1β after operation: Compared with value of the average optical density of TNF-α and IL-1β of group C, those of groups D, E and G were obviously different after operation(<i>P</i><0.05). Compared D with E, G groups, there was no significant difference between those group after operation(<i>P</i>>0.05).<p>CONCLUSION: 1)Compared with androgen, BOED has the similar effect of depressing the expression of inflammatory factors. But its effect is slightly weaker than the androgen; 2)The depressing effect of medium and high concentration BOED are better than low concentration. Compared with medium and high concentration of BOED, there was no significant difference between two groups.

Chemical Constituents from Buddleja officinalis / 中国药学杂志

OBJECTIVE: To study the chemical constituents from the flower buds and inflorescences of Buddleja officinalis. METHODS: Various chromatographic techniques such as silica gel and Sephadex LH-20 chromatography were used in this experiment. RESULTS: Sixteen compounds were isolated from the medicinal plant, and their structures were identified as follows acacetin(1), apigenin(2), luteolin(3), acacetin-7-O-glucoside(4), cosmosiin(5), luteolin-7-O-glucoside(6), acacetin-7-O-glucuronide(7), apigenin-7-O-glucuronide(8), linarin(9), luteolin-7-O-rutinoside(10), neobudofficide(11), acteoside(12), crocin III(13), crocin II(14), crocin I(15), and N1, N5, N10-(E)-tri-p-coumaroylspermidine(16). CONCLUSION: Compounds 4, 7, 8, 14-16 are obtained from the medicinal plant for the first time, and compounds 7, 8, 14-16 are obtained from Loganiaceae for the first time.

Actividad antioxidante en infusiones, tinturas y aceites esenciales de especies nativas de la Patagonia Argentina / Antioxidant activity in teas, tinctures and essential oils of native species from Patagonia Argentina

Introducción: las plantas aromáticas y medicinales son una fuente potencial de componentes antioxidantes. La Patagonia Argentina presenta un ambiente diverso en especies nativas, las cuales deberían ser estudiadas en mayor profundidad debido a su potencial farmacéutico, así como para contribuir a fomentar su conservación. Objetivos: estudiar la actividad antioxidante de infusiones, tinturas y aceites esenciales de las siguientes especies nativas de la Patagonia Argentina: Acantholippia seriphioides (A. Gray) Moldenke, Adesmia boronioides Hook. f., Buddleja globosa Hope, Fabiana imbricata Ruiz & Pav., Solidago chilensis Meyen. Identificar los componentes volátiles presentes en los aceites esenciales. Métodos: se obtuvieron infusiones y tinturas por la guía de las normas de la Farmacopea Argentina VI edición. Los aceites esenciales se obtuvieron por hidrodestilación con un aparato tipo Clevenger. El análisis de los componentes volátiles se realizó mediante cromatografía de gases-espectrometría de masas. Los ensayos de actividad antioxidante se realizaron por el método del difenil-picrilhidrazilo. Resultados: las especies presentaron el siguiente orden de actividad antioxidante: B. globosa > S. chilensis ≥ F. imbricata ≥ A . seriphioides > A. boronioides. Las infusiones de B. globosa, S. chilensis y A. seriphioides, presentaron una actividad antioxidante similar a Camellia sinensis (L.) Kuntze ("té verde") y superior a Ginkgo biloba (L.) Mant (especies reconocidas por su alto contenido de antioxidantes). La actividad encontrada para el aceite esencial de A. seriphioides se deba a sus contenidos en timol y carvacrol. En cuanto a la actividad de S. chilensis podría adjudicarse a su alto porcentaje de limoneno. Conclusiones: este trabajo es el primero que estudia la actividad antioxidante de plantas medicinales y aromáticas en la región noroeste de la Patagonia Argentina; los resultados obtenidos demuestran que las especies estudiadas de dicha región son una fuente rica en compuestos antioxidantes y de potencial valor como suplemento dietario(AU)

Introduction: medicinal and aromatic plants have potential as sources of antioxidant compounds. There is a great diversity of native species in Patagonia Argentina. It is worthy to study them because of its pharmaceutical potential and to help promote conservation. Objectives: to analyze antioxidant activities of herbal teas, tinctures and essential oils of native species from Patagonia Argentina: Acantholippia seriphioides (A. Gray) Moldenke, Adesmia boronioides Hook. f., Buddleja globosa Hope, Fabiana imbricata Ruiz & Pav., Solidago chilensis Meyen. Identify essential oils compounds. Methods: infusions and tinctures were obtained according to Pharmacopoeia Argentina VIth edition. The essential oils were obtained by hydrodistillation in a Clevenger apparatus. Volatiles compounds were analyzed by gas chromatography-mass spectrometry. Antioxidant activity assays were performed by difenil-picrilhidrazilo method. Results: antioxidant activity order was: B. globosa > S. chilensis ≥ F. imbricata ≥ A. seriphioides > A. boronioides. Infusions of B. globosa, S. chilensis and A. seriphioides presented an antioxidant activity similar to Camellia sinensis (L.) Kuntze ("green tea") and higher than Ginkgo biloba (L.) Mant. The A. seriphioides essential oil activity was probably obeyed to thymol and carvacrol presence. S. chilensis activity could be owing to its high limonene content. Conclusions: this study is the first report about antioxidant activity of medicinal and aromatic plants in the northwest region of Patagonia Argentina. The results showed that analyzed species are a rich source of antioxidant compounds and have potential value as a dietary supplement(AU)

Chemical Constituents from Buddleja officinalis and Their Inhibitory Effects on Nitric Oxide Production

Bioactivity-guided fractionation of a methanolic extract of Buddleja officinalis led to the isolation of two monoterpenes, crocusatin M (1), crocusatin C (2), a flavonoid, acacetin (3), three lignans, lariciresinol (4), pinoresinol (5), and syringaresinol (6), and two triterpenoidal saponins, mimengoside B (7) and songarosaponin A (8). The structures of isolates were identified based on 1D-, 2D-NMR, and MS data analysis. All isolates were tested for their inhibition on LPS-induced NO production in RAW 264.7 cells. As a result, mimengoside B (7) and songarosaponin A (8) showed a mild inhibitory activity of NO production.

HPLC-ELSD for determining contents of three triterpenoidal saponins in fruits of Buddleja lindleyana from different habitats / 中国中药杂志

To establish an HPLC-ELSD method for the quantification of triterpenoids in the fruits of Buddleja lindleyana. The RP-HPLC-ELSD method was used for the determination of triterpenoids in B. lindleyana fruits, which were collected from different habitats. The column used was a packed with 5 μm stationary phase Waters SunFireTM C₁₈ (4.6 mm×150 mm, 5 μm). The mobile phase consisted of Methanol-water(82∶18) at a flow rate of 1 mL•min⁻¹. Column temperature： 30 ℃. ELSD conditions： drift tube temperature： 106 ℃; carrier gas (nitrogen) flow rate： 1.5 L•min⁻¹; amplification factor： 1. The calibration curves showed good linear relationship on a range from 0.702 to 28.08 μg(r=0.999 2) for Clinoposaponin III, 0.390 to 15.60 μg(r=0.998 9) for Desrhamnoverbascosaponin and 0.192 to 7.68μg(r=0.999 0) for Mimengoside I. The average recovery rate(n=6) were 99.41%, 99.08% and 98.67% and it's RSD were 0.86%, 1.56% and 1.80%. This method can be used to determine the contents of triterpenoids in the fruits of Buddleja lindleyana for its simplicity, accurateness and reliability.

Enzyme kinetic analysis of Oncomelania hupensis exposed to active ingredient of Buddleja lindleyana (AIBL) / 中国血吸虫病防治杂志

Objective To analyze the enzyme kinetics of active ingredient of Buddleja lindleyana(AIBL)against Oncomela?nia hupensis,the intermediate host of Schistosoma japonicum. Methods O. hupensis snails were placed in 1 000 ml of 3.55 mg/L AIBL solution for 24,48 h and 72 h,respectively,and the enzyme kinetics of alanine aminotransferase(GPT)was deter?mined by Reitman?Frankel assay,lactate dehydrogenase(LDH)by the chemical inhibition lactic acid substrate method,alka?line phosphatase(AKP)by the disodium phenyl phosphate colorimetric method,acetylcholine esterase(AChE)and malate de?hydrogenas(MDH)by ELISA,and succinate dehydrogenase(SDH)by the phenazine methyl sulfate reaction method(PMS)in the soft tissues of O. hupensis before and after AIBL treatment. Results Following exposure to 3.55 mg/L AIBL solution for 24 h, the GPT,LDH,and AKP activities significantly improved in the soft tissues of O. hupensis,while the SDH and MDH activities were significantly lowered in the head?foot and liver. However,AIBL treatment did not cause significant effect on AChE activity in O. hupensis. Conclusions AIBL causes significant damages to O. hupensis liver and can efficiently act on anaerobic and aer?obic respiration loci,which will hinder energy metabolism,and cause inadequate energy supply in cells used for normal secre?tion,eventually leading to O. hupensis death.

Chemical constituents from stems and leaves of Buddleja lindleyana Fort / 国际药学研究杂志

Objective To investigate the anti-H5N1 activities and chemical constituents from stems and leaves of Buddleja lindleyana Fort. Methods Constituents were separated through AB-8 macroporous resin, chromatography of silica gel, Sephadex LH-20 and recrystallization. Structures of the compounds were identified by analysis of physicochemical properties and spectral data. Results Ten compounds were isolated and identified as linarin(l), rutin(2), luteolin(3), quercetin(4), apigenin(5), hesperetin (6), salidroside (7), oleanolic acid (8), ß-sitosterol (9), and daucosterol (l0), respectively. Conclusion Compounds 2-7 were obtained from this plant for the first time.

Chemical constituents from stems and leaves of Buddleja lindleyana Fort / 国际药学研究杂志

Objective To investigate the anti-H5N1 activities and chemical constituents from stems and leaves of Buddleja lindleyana Fort.. Methods Constituents were separated through AB-8 macroporous resin, chromatography of silica gel, Sephadex LH-20 and recrystallization. Structures of the compounds were identified by analysis of physicochemical properties and spectral data. Results Ten compounds were isolated and identified as linarin(1), rutin(2), luteolin(3), quercetin(4), apigenin(5), hesperetin (6), salidroside (7), oleanolic acid (8),β-sitosterol (9), and daucosterol (10), respectively. Conclusion Compounds 2-7 were obtained from this plant for the first time.

Estrous cycle disruptor effect of an ethanolic extract from Buddleja globosa leaves and its main component (verbascoside) / Efecto disruptor del ciclo estral de un extracto etanólico de hojas de Buddleja globosa y de su componente principal (verbascósido)

The estrous cycle disruptor effect of an ethanolic extract (EMATst) from Buddleja globosa leaves and standardized in its main component (verbascoside) was determined in rats after the subcutaneous administration of EMATst. Binding of EMATst and verbascoside to the estrogen receptor (ER) of EMATst and verbacoside was also measuredestablished. EMATst produced a significant alteration inof the estrous cycle only at the highest dose (10-5 M), which could be attributed to an antiestrogenic effect. The Bbinding of EMATst and verbascoside to the ER was competitive and occurred in concentrations 1000 times greater than that of 17beta-estradiol.

El efecto disruptor del ciclo estral de un extracto etanólico (EMATst) obtenido a partir de las hojas de Buddleja globosa y estandarizado en su componente mayoritario (verbascósido) fue determinado en ratas después de la administración subcutánea de EMATst. Se estableció además la unión al receptor estrogénico (RE) tanto de EMATst como de verbascósido. EMATst sólo a la dosis más alta (10-5M) produjo una alteración significativa del ciclo estral, lo que podría atribuirse a un efecto antiestrogénico. La unión al RE de EMATst y verbascósido se produjo a concentraciones 1000 veces mayor que el 17beta-estradiol y de forma competitiva.

Effect of different provenances and production conditions on antioxidant properties in Buddleja globosa leaves / Efecto de diferentes procedencias y condiciones de cultivo sobre propiedades antioxidantes en hojas de Buddleja globosa

Buddleja globosa Hope (matico) is a medicinal shrub native to Chile whose leaves have been traditionally used for wound and ulcer healing, pathologies associated to oxidative stress. Matico leaves display a high content of polyphenols, compounds with recognized antioxidant capacity, which may contribute to its therapeutic properties. Several factors, however, can modify the polyphenol content of matico leaf extracts, including plant material, production techniques, provenances, leaf age, harvest time, irrigation, and desiccation procedures. Thus, standardized leaf extracts prepared with plants from different provenances and harvest conditions were compared in terms of polyphenol content and their protecting antioxidant effects on rat liver microsomal lipids and thiol groups. All factors tested, but irrigation, changed both polyphenol content and antioxidant properties of matico extracts; water stress only affected their antioxidant properties without changing their polyphenol content. Correlation between polyphenol content and lipid peroxidation inhibition was only significant in the provenance study.

Buddleja globosa Hope (matico) es un arbusto medicinal nativo de Chile cuyas hojas han sido utilizadas en la medicina tradicional como cicatrizante en caso de patologías relacionadas con el estrés oxidativo. Las hojas tienen un alto contenido de polifenoles, compuestos con reconocidos efectos antioxidantes relacionados con la inhibición de la lipoperoxidación. Varios factores pueden afectar a su contenido, entre ellos el origen de la planta, edad de la hoja, momento de cosecha, riego y métodos de secado. En extractos estandarizados preparados de hojas de diferentes tratamientos se compararon el contenido de polifenoles y los efectos antioxidantes protectores de lipidos y grupos tioles microsomales. Todos los ensayos de cultivo y postcosecha mostraron diferencias significativas entre los tratamientos, excepto para la inhibición de lipoperoxidación en el tratamiento de riego. Plantas de diferente origen muestran que el contenido de polifenoles en las hojas es determinado genéticamente y sufre variaciones por efectos ambientales.

Effects of extract of Buddleja officinalis eye drops on androgen receptors of lacrimal gland cells of castrated rats with dry eye / 国际眼科杂志(Guoji Yanke Zazhi)

AIM: To evaluate the effects of the extract of Buddleja officinalis eye drops in basic tears secretory volume, tear film stability, expression of androgen receptors(AR) in castrated rats with dry eye, and to investigate the therapeutic effects of extract of Buddleja officinalis on dry eye caused by gonadal hormones level imbalance. METHODS:A total of 45 Wistar masculinity rats were divided at random into 9 groups, including normal group(A1,A2 and A3), model group(B1,B2 and B3), therapy group with extract of Buddleja officinalis eye drops(C1,C2 and C3). The "1" stood for being fed for 1 month, and "2" for 2 months, and "3" for 3 months. The dry eye model was established with orchiectomy on group B,C. Group C was treated with Buddleja officinalis extract eye drops for one month. All rats were checked with Schirmer Ⅰ test (SⅠt) and tear film break-up time (BUT). Expression of AR was analyzed by flow cytometer(FCM). RESULTS:The SⅠt value of group C was significantly higher than that of group B (P<0.01) and the BUT value of group C was significantly longer than that of group B (P<0.01), which indicated the eye drop could significantly keep basic tears secretory volume and tear film stability. And the expression of AR of group C was much higher than that of group B,which showed that available composition of the eye drops maybe display androgen-like activity.CONCLUSION:The main components of extract of Buddleja officinalis is the flavonoids which could significantly inhibit happening of dry eye of rat after androgen level lowered. Its mechanism is like androgen's and it could display androgen-like activity to keep basic tears secretory volume and tear film stability.

Buddlejaceae endémicas del Perú

La familia Buddlejaceae es reconocida en el Perú por presentar dos géneros y 16 especies (Norman, 2000; Ulloa Ulloa et al., 2004), principalmente arbustos. En este trabajo reconocemos dos especies endémicas en el género Buddleja. Estos taxones endémicos se encuentran en las regiones Mesoandina, Puna Húmeda y Seca y Páramo, entre los 2650 y 3900 m de altitud. Una de estas dos especies se encuentra representada dentro del Sistema Nacional de Áreas Naturales Protegidas por el Estado.

The Buddlejaceae are represented in Peru by two genera and 16 species (Norman, 2000; Ulloa Ulloa et al., 2004), mainly shrubs. Here we recognize two endemic species, both in the genus Buddleja. These endemic taxa are found in Mesoandean, Humid and Dry Puna, and Paramo regions, between 2650 and 3900 m elevation. One species has been registered within the Peruvian parks system.