Effect of Extract of Ginkgo Biloba(EGb)on Cell of Neonatal Rat Cardiocyte Hypertrophy Induced by Prostaglandin F2 Alpha / 医学研究杂志

Objective To investigate the effects and mechanisms of extract of ginkgo biloba(EGb)on the cell of cardiocyte hypertrophy induced by prostaglandin F2 alpha.Methods normal cells were used as negative control,and spontaneously PGF2? and EGb were used as experimental groups.The cells were isolated and cultured on cultured neonatal rat cardiocyte by PGF2?,cardiocyte hypertrophy was determined by the cell surface and the total protein of cells;the level of intracellular ROS measured by the fluorescence microscope.Results In cardiocyte hypertrophy,the cell surface,the level of intracellular ROS and the total protein of cells increased significantly in cutured neonatal rat cardiac treated with PGF2?.Compared with cells treated with PGF2?,EGb(40?g/ml,80?g/ml,100?g/ml)inhibited cardiocyte hypertrophy by PGF2?-induced,decreased in the cell surface by 19%,27%,33% and in the total protein of cells by 21%,39%,47% respectively(all P

Inhibitory effect of nitric oxide on hypertrophic response of cardiomyocytes stimulated by stretch in vitro / 第三军医大学学报

3?10 -4 mol/L), and the role was persistent if its concentration was kept. Meanwhile, the increase of intracellular free Ca 2+ induced by stretch was also inhibited by NO. Conclusion Cardiocytes hypertrophic response stimulated by stretch could be inhibited by nitric oxide. The inhibitory effect might be due to the decrease of the concentration of intracellular free Ca 2+ .