Content Determination of Isoniazid in Isoniazid Tablets by Microfluidic Chip with Contactless Conductivi-ty Method / 中国药房

OBJECTIVE:To establish a method for the content determination of isoniazid in Isoniazid tablet. METHODS:Mi-crofluidic chip with contactless conductivity detection was adopted. The effects of electrophoresis parameters,such as the variety, concentration,pH,additive types and dosage,injection time and separation voltage on the separation detection were explored. RE-SULTS:The optimized buffer system was 1.86 mmol/L citrate-0.14 mmol/L sodium citrate buffer solution (pH3.0),no additive , the injection time was 20 s and the separation voltage was 2.5 kV. In the optimal conditions,the linear range of isoniazid was 10-200 μg/mL(r=0.9993);the limits of quantification and detection were 8.0 μg/mL and 2.4 μg/mL;RSDs of precision,stability and reproducibility tests were lower than 2.0%;recovery was 97.8%-102.9%(RSD=1.9%,n=9). CONCLUSIONS:The method is rapid and simple,and suitable for the content determination of isoniazid in Isoniazid tablet.

Recent analytical strategies on "Date-Rape" Drugs and its metabolites.

Recently, much attention has been given to develop simple analytical methods for screening wide range of date-rape drugs. Drug abuse that changes human behavior and guides to crimes has become a serious problem throughout the world. The determination of various classes of abused drugs is an important in many fields of analytical toxicology, such as forensic analysis and antidoping analysis. The detection method must provide more accurate and reliable results from the test specimens. In this regard, attempt is made in this review to summarize the recent applications of these methods in keeping track with complexity of the sample matrices. The following topics are discussed under the headings of different sampling approaches for screening the drugs related to drug facilitated sexual assault and more commonly utilized analytical methods such as liquid chromatography, gas chromatography and capillary electrophoresis separation techniques coupled with mass spectrometry detector. Therefore, relentless efforts should continue to be deployed toward research and development in low cost, fast and efficient ways of detecting illicit drug from all kind of specimens for reliable and more accurate results.

Simple and Efficient Enantioseparation of Ofloxacin by Capillary Electrophoresis with Contactless Conductivity Detedction Using Binary Chiral Selectors / 分析化学

Novel method of enantioseparation of ofloxacin by capillary electrophoresis with capactively coupled contactless conductivity detection (C~4D) using binary chiral selectors,hydroxypropyl cyclodextrin and hydroxypropyl methyl cellulose ( HP-β-CD and HPMC) ,was developed. The enantiomers of ofloxacin were baseline separated with an uncoated fused-silica capillary(45 cm×50 m i. d. ,L_(effi) = 40 cm)under the optimum separate conditions, 20 mmol/L HAc +6 mmol/L NaAc + 12 mg/L HPMC +35 mmol/L HP-β-CD,separation voltage +13 kV,electrokinetic injection 11 kV×10 s. The calibration curve of the enantiomers showed good linearity in the range from 0. 8 mg/L to 40 mg/L with LOD =0. 3 mg/L. Effects of several factors on resolutions,such as the composition of the running buffer,the pH value,separation voltage,injection method and the parameters of C~4D detector,were investigated. The efficacy of the HP-β-CD and HPMC were discussed. The proposed method has been successfully applied to monitor the enantiomer of ofloxacin contents in the commercial Ofloacin Tablets and Levofloxacin Hydrochloride Tablets,and was demonstrated simple,rapid and reproducible.