Superoxide dismutase and lipid hydroperoxides in blood and endometrial tissue of patients with benign, hyperplastic and malignant endometrium

Epidemiological and experimental data point to involvement of oxygen derived radicals in the pathogenesis of gynecological disorders, as well as in cancer development. The objective of the present study was to examine changes in activities and levels of copper/zinc superoxide dismutase (CuZnSOD) and lipid hydroperoxides (LOOH) in blood and endometrial tissue of patients diagnosed with uterine myoma, endometrial polypus, hyperplasia simplex, hyperplasia complex and adenocarcinoma endometrii. The results of our study have shown decreased SOD activities and unchanged SOD protein level in blood of all examined patients in comparison to healthy subjects. Decrease of both SOD activity and level was found in endometrium of patients with hyperplasia simplex, hyperplasia complex and adenocarcinoma in comparison to women with polypus or myoma. LOOH level was elevated in both tissues of patients with hyperplasiaor adenocarcinoma in comparison to healthy subjects or patients with benign diagnosis. Our findings suggest that the decrease in SOD activity and level, as well as the increase in LOOH level, in patients with gynecological disorders, render these patients more susceptible to oxidative damage caused by reactive oxygen species (ROS). An imbalance in ROS formation and SOD level may be important in the pathogenesis and/or perpetuation of tissue damage in gynecological patients. Since evidence suggests that SOD may be a therapy target for cancer treatment, our findings provide a basis for further research and options for clinical applications.

Resultados epidemiológicos e experimentais apontam para o envolvimento dos radicais derivados do oxigênio na patogênese das moléstias ginecológicas, assim como no desenvolvimento do câncer. O objetivo do presente estudo foi o de examinar as alterações nas atividades e níveis de Cu/Zn superóxido dismutase (CuZnSOD) e hidroperóxidos lipídicos (LOOH)no sangue e tecido endometrial de pacientes diagnosticados com mioma uterino, pólipo endometrial, hiperplasia simplex, hiperplasia complex e adenocarcinoma do endométrio. Os resultados de nosso estudo mostraram atividades de SOD diminuídas e nível de SOD proteína inalterado no sangue de todos os pacientes examinados em comparação a indivíduos saudáveis. Diminuição de ambos, atividade de SOD e nível protéico, foram encontrados no endométrio de pacientes com hiperplasia simplex, hiperplasia complex e adenocarcinoma em comparação às mulheres com pólipos e/ou mioma. O nível de LOOH estava elevado em ambos os tecidos de pacientes com hyperplasia e adenocarcinoma em comparação a indivíduos saudáveis ou pacientes com diagnóstico benigno. Nossos resultados sugerem que um decréscimo na atividade e nível protéico de SOD, assim como um incremento no nível de LOOH, em pacientes com desordens ginecológicas, tornam esses pacientes mais susceptíveis ao dano oxidativo causado pelas espécies reativas de oxigênio (ROS). Um desequilíbrio na formação de ROS e no nível de SOD pode ser importante na patogênese e/ou perpetuação do dano tecidual em pacientes ginecológicos. Desde que existe evidência de que SOD pode ser um alvo para terapia de câncer, nossos resultados fornecem uma base para futura pesquisa e opções para aplicações clínicas.

Distribution and Expression of Superoxide Dismutase in Pediatric Tonsils and Adenoid / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES: B-lymphocytes and other phagocytes which infiltrate into inflammatory sites in pathologic condition are able to make reactive oxygen species such as superoxide free radicals (SFRs) which have toxic effects on foreign bodies including bacteria as well as normal eukaryotic cells. To prevent harmful effects of SFRs, eukaryotic cells can produce antioxidant enzymes such as superoxide dismutase (SOD) and catalase. The purpose of this study is to investigate the protective system of pediatric palatine tonsils and adenoids against the SFRs and to evaluate the clinical factors including otitis media with effusion that are related to the expressions and activities of copper zinc superoxide dismutases (CuZnSODs) in these tissues. MATERIALS AND METHOD: Twenty-one children who were operated for enlarged tonsils and adenoid with or without otitis media with effusion (OME) from December 1999 to Feburary 2000 were studied. Physcial fidings were evaluated and history-taking was carried out. RT-PCR and immunohistochemistry stain against CuZnSOD were conducted. RESULTS: Immunohistochemistry showed that CuZnSODs were found in the crypt epithelium, mucous membrane, mantle zone and in the extrafollicular area of pediatric adenoids as well as palatine tonsils. There were significant correlations between the presence of OME, paranasal sinusitis and the expression of CuZnSOD of adenoid (p<0.05), the frequency of tonsillitis, recent tonsillitis and the expression of CuZnSOD of tonsils (p <0.05). CONCLUSION: In pediatric tonsils and adenoids, the mechanism of tissue protection against infection and frequent inflammatory reactions may be closely related to the CuZnSODs expression.

Immunohistocheinical Localization of Copper-Zinc Superoxide Dismutase in Human Hepatocellular Carcinoma and Its Surrounding Tissues / 第二军医大学学报

By using protein A-gold labeling probe, copper-zinc superoxide dismutase (Cu-Zn SOD)in paraffin-embedded sections of 24 cases of human hepatocellular carcinoma (HCC) and its surrounding liver tissues was immunolocalizied The experimental results showed that in a good many of HCCs and its surrounding liver tissues, immunostaining of Cu-Zn SOD appeared differently positive, but in 2 cases of grade Ⅱ differentiation, massive pattern of HCC, Cu-Zn SOD immunostaining was negative. In one hepatoma was clear cell carcinoma, and in another one tumor cells followed obvious fatty degenera tioa Two problems that what was significance of Cu-Zn SOD in HCQ and a few cellular nuclei labeled for Cu-Zn SOD are disscussed.