DNA content, the expression of VEGF and p53 in pleural effusion can predict the cure and prognosis / 肿瘤

Objective: To investigate the association of nuclear DNA content and vascular endothelial growth factor (VEGF) and p53 expression with therapeutic response of malignant pleural effusion and their value in predicting the prognosis of wet lung cancer. Methods: The survival periods of 43 lung cancer patients with pleural effusions were followed up. The DNA content of 39 patients was measured by the image cytometry (ICM) and the expression of VEGF and p53 was determined by Envision immunohistochemical method. Twenty-nine patients were given bleomycin or interleukin-2 intrathoracically after drainage. Results: DNA aneuploid had a tendency to correlate with therapeutic efficacy of malignant pleural effusion (P = 0. 054). Cox multivariate analysis showed that only p53 expression was independent prognostic factor for lung patients with pleural effusion (P = 0.05). The median survival time of patients was (10.4 ± 3.5) months for p53-negative patients and (2.8 ± 0.6) months for p53-positive patients (log rank = 0.013 2). One-year survival rate was 17.7% for p53-negative patients and 0% for p53-positive patients. Conclusion: DNA content measured by ICM tended to correlate with the therapeutic efficacy of malignant pleural effusion; p53 expression is a unique independent prognostic factor for lung cancer patients with pleural effusions.

Flow cytometric evaluation on the age-dependent changes of testicular DNA contents in rats

An age-dependent cellular change of DNA contents in the testis of Sprague-Dawley rats was investigated by flow-cytometric method. Testicular cell suspensions at the age of 4, 5, 6, 7, 8, 10, 12, 16 and 26 weeks were prepared and stained with propidium iodide. The relative proportions in the number of mature and immature haploid (1n), diploid (2n), S-phase and tetraploid (4n) cells were calculated. The proportion in the number of mature haploid cells was sharply increased to the age of 10 weeks (about 38%), thereafter increased slightly to the level of 42% at the age of 26 weeks. The proportion of immature haploid cells was dramatically increased to the age of 6 weeks, then maintained at the level of 20 to 30% thereafter. The proportion of diploid cells was 64% at the age of 4 weeks, then decreased gradually through the age of 26 weeks. The proportion of S-phase cells was increased to the age of 4 weeks, then maintained at a plateau level to the age of 26 weeks. The proportion of tetraploid cells were about 26% at the age of 4 weeks, then decreased gradually to the age of 26 weeks. These results suggest that the proportions of testicular cells may depend on the age of the rat and that the flow cytometric method may be useful in the evaluation of the spermatogenic status with regard to accuracy and sensitivity.

Effect of ethylene glycol monoethyl ether on the spermatogenesis in pubertal and adult rats

The effects of ethylene glycol monoethyl ether (EGEE) on testicular cell populations in pubertal (5 weeks old) and adult (9 weeks old) male rats were investigated by a flow cytometric method. A total of 50 rats (in number, 25 pubertal and 25 adult rats) was divided into 5 experimental groups including 0 (control), 50, 100, 200, and 400 mg EGEE/kg of body weight. The animals were administered by gavage for 4 weeks. In adult rats, the treatment of EGEE at the dose of 400 mg/kg of body weight decreased significantly the populations of haploid, while it increased those of diploid and tetraploid cells. In pubertal rats, the treatment of EGEE at the dose of 400 mg/kg of body weight caused only minimal changes in the relative percent of testicular cell types. These results suggest that the effects of EGEE on testicular function in pubertal rats appear to be less pronounced than in adult rats.

Relationship between pathological grade,DNA content and MRI-appearance in brain astrocytic tumors / 中国病理生理杂志

AIM: To study the correlation between DNA index(DI),5cER,MRI appearance and the malignant grades of brain astrocytic tumors.METHODS: After Feulgen staining,40 paraffin samples of astrocytic tumors were observed about DI and 5cER by image analysis,MRI appearances were reviewed,including edema around tumor,clearity of boundary,equality of MRI singal,enhancement of tumor,shift of medline,necroses and cyst,then compared them with pathological grade and survival rate.RESULTS: There was a significant positive correlation between DI,5cER,MRI appearance and pathological grade,negative correlation between DI,5cER,MRI appearance and survival rate.CONCLUSION: DI,5cER and MRI appearance are very important in judging pathological grade and patient's prognosis.When 5cER mean of astrocytic tumor exceeds 20,the tumor should be judged as glioblastoma.