Comparison of three kinds of catheterization in the treatment of lacrimal duct obstruction / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To compare the therapeutic effects of lacrimal duct retrograde catheterization, double loop antegrade catheterization, and lacrimal duct U-shaped catheter for patients with lacrimal duct obstruction.<p>METHODS: Totally 108 patients with lacrimal duct obstruction who were treated in our hospital from July 2015 to June 2018 were selected as subjects and prospectively studied. They were randomly divided into three groups: A(36 cases), B(36 cases), and C(36 cases). In group A, lacrimal duct retrograde catheterization was performed. Group B was treated with double-loop antegrade catheterization. Group C was treated with lacrimal duct U-shaped catheterization. All patients were followed up for 6mo. The intraoperative basic conditions(Lntraoperative blood loss, operation time, hospital stay), treatment efficacy, and the occurrence of complications and recurrence of lacrimal duct obstruction of the three groups were compared. <p>RESULTS: There were significant differences in the amount of intraoperative blood loss, operation time and hospital stay between the three groups(<i>P</i><0.05). The total effective rate of treatment in group A was 91.7%, which was lower than 94.4% in group B and 97.2% in group C, but the difference in total effective rate between the three groups was statistically significant(<i>P</i>>0.05). The complication rate was 11.1% in group A, 5.6% higher than that in group B, and higher than 2.8% in group C(<i>P</i>=0.010). The recurrence rate of lacrimal duct obstruction in group A was 9.1%, higher than 5.9% in group B, higher than that in group C(<i>P</i>=0.029).<p>CONCLUSION: Retrograde catheterization of the lacrimal duct, double-loop antegrade catheterization, and lacrimal duct U-shaped tube for the treatment of patients with lacrimal duct obstruction have higher therapeutic effects, postoperative complications and less recurrence of lacrimal duct obstruction, but lacrimal passage Retrograde catheterization can reduce the amount of intraoperative blood loss, operation time, and U-shaped catheterization of the lacrimal duct can reduce the length of hospital stay, and can be widely used in clinical practice.

Design and Evaluation of Online/offline Interface Based on Double Loop for Two-dimensional Chromatography / 分析化学

By using a double loop technique based on a set of mechanical transmission components and a flow path system,a double function of injection and fraction collection was realized. On the basis of double loop technique,a novel type of online/offline interface for two-dimensional liquid chromatography was designed to construct an efficient separation system coupling two different separation modes with a higher peak capacity, and the functions of the interface were evaluated. By means of connecting the interface to an HPLC system, the multiple functions of injection, fraction collection and injection again were fulfilled for preparation and purity analysis of 4 kinds of aromatic compounds. As for combination of 2 sets of HPLC system,5 kinds of protein samples were separated preliminarily by strong cation exchange chromatography, and the components difficult to separate were collected and injected into reversed phase chromatographic system for further separation. Furthermore, the interface was applied to coupling two chromatographic systems in both strong cation exchange mode and microcolumn reversed-phase mode for the two-dimensional separation of bovine serum albumin enzymatic digest. When 1 mAU was set as the integral threshold,a total number of 292 peaks were identified. With the help of the online/offline interface, the preparation of microscale samples, fine separation of hardly separated samples and two-dimensional separation of complex samples were achieved flexibly. The result indicated that the system was a potent tool for the construction of two-dimensional chromatographic system and separation research.

Preparation and performance research of target nmr contrast agent gd-dota-e-2 / 中国药学杂志

OBJECTIVE: To prepare Gd-DOTA-E-2 targeted contrast agent and evaluate its relaxation properties, cytotoxicity and targeting. METHODS: E-2 was synthesized by F-moc solid phase method, covalently bonded with chelating agent DOTA, meanwhile ultilizing chelating ligand Gd3+ to prepare MRI contrast agent Gd-DOTA-E-2. The structure was characterized by ESI-MS and NMR, and the properties were evaluated by relaxation properties, MTT and mouse MRI contrast assay. RESULTS: ESI-MS and NMR RESULTS showed that Gd-DOTA-E-2 was synthesized successfully, and its relaxation efficiency(k=9.551 mmol-1•L•s-1) is 1.84 times more efficient than a commercially available contrast agent of Gd-DOTA; MTT RESULTS showed that the toxicity of Gd-DOTA-E-2 cell is lower than that of Gd-DOTA; the experimental RESULTS in MRI angiography showed that Gd-DOTA-E-2 is possessing of targeting and can image clearly. CONCLUSION: Gd-DOTA-E-2 is a kind of MRI contrast agent with good stability, low cytotoxicity and targeted properties in vivo,which is valuable in research and application.

Application of individualized nursing and double loop learning in early onset severe preeclampsia / 中国实用护理杂志

Objective Combining the concept of double loop learning in enterprise management with individualized nursing practice,the application value of this method in nursing care of patients with early onset severe preeclampsia(ESPE)was analyzed. Methods Patients with ESPE were selected from January 2014 to December 2016, which were divided into observation group and control group by random digits table method. The patients in observation group were given individualized nursing care with the idea of double loop learning,while the patients in control group received routine nursing care. The hospital admission, maternal pregnancy outcome and nursing satisfaction of the patients were compared between two groups. Results The observation group included 96 cases and the control group included 101 cases. There was no significant difference between two groups in age, gestational age, systolic blood pressure, diastolic blood pressure, and 24 hours urine protein(P>0.05).There were 87 cases of cesarean section in the observation group,while 94 cases in the control group, and there was no significant difference(P>0.05). The gestational weeks,the incidence of neonatal asphyxia,the nursing satisfaction was(35.5±2.7)weeks,5.2%(5/96), 90.6%(87/96)in the observation group and(34.1±2.0)weeks, 15.8%(16/101),72.3%(73/101)in the control group, the difference was statistically significant(t=4.149, χ2=5.843, 10.862, P<0.05 or 0.01). Conclusions In the ESPE nursing management, the individualized nursing and double loop learning can improve the child pregnancy outcomes and nursing satisfaction.