Introduction of SDL in Department of Anatomy: Evaluation of Learning and SDL Readiness

Background: Self-directed learning (SDL) is a process in which individuals assume the responsibility of identifying their learning needs, setting goals, locating sources, developing and implementing proper strategies, and evaluating the outcomes of learning both individually and collectively. The advantages of SDL include, but not limited to, gaining more autonomy in learning, having higher motivation for it, acquiring lifelong learning skills, and developing greater self-control, self-confidence, and self-management. SDL has become popular in medical curricula and has been advocated as an effective learning strategy for medical students to develop competence in knowledge acquisition. Aim and Objectives: To introduce self directed learning in the Department of Anatomy, to encourage faculty members to accept SDL as a Teaching Learning method and encouraging students to develop an attitude of self directed learning & to become lifelong learners. Methodology: After proper sensitization of the faculty & students regarding SDL, two sessions were conducted during the Anatomy Dissection Hall timings of the First Year students. The performance of the students, in the SDL topics was assessed using the MCQ & SAQ tests. Feedback regarding the SDL methodology was taken both from the students as well as the faculty. Also the Questionnaires were given to students applying Fischer’s Self Directed Learning Readiness Scale (SDLRS) & Jefferson Scale of Life Long learning –Health Profession Version (JeffSLL-HSP) & the results were statistically analyzed. Results: A total of 126 MBBS students of the first semester participated in SDL session and most students scored more than mean score in the tests after SDL sessions, there was a fourfold increase in the number of students scoring more than 80% in the two SDL tests. Also participating students showed high readiness towards SDL and were oriented to become Life Long Learners. Conclusions: SDL can be effectively implemented in the Department of Anatomy and it motivates students to become life long learners.

Mechanisms of Gancao Xiexin Decoction on protecting intestinal epithelial cell barrier under stress state by regulating PERK-elF2α-CHOP signaling pathway / 中国药理学通报

Aim To observe the protective effect of Gancao Xiexin Decoction (GXD) on intestinal epithelial cells (IECs) under endoplasmic reticulum stress from the perspective of PERK-elF2α-CH0P signaling pathway. Methods Caco-2 cells were cultured and divided into normal control group (NC), model control group(MC), low-dose GXD group(GXD-L), mediumdose GXD group (GXD-M) and high-dose GXD group (GXD-H). Models of the stress epithelial cells were induced by tunicamycin(Tm), and GXD groups were treated with GXD contained serum at the same time. The cell survival rate was assessed by CCK-8 method, the cell apoptotic rate and cell cycle distribution were determined by flow cytometry, and the cell barrier permeability was determined by TEER and FITC-dextran method; the expression levels of core proteins of PERK-elF2α-CH0P signaling pathway were detected by Western blot. Results Compared with MC group, GXD intervention could improve cell survival rate(P < 0. 05), reduce their apoptotic rate(P <0. 01), relieve cell cycle arrest(P < 0. 01), improve cell barrier permeability by increasing cell TEER value (P < 0. 01) and decreasing FITC-dextran concentration (P < 0.05), and the levels of p-PERK, p-elF2α, ATF4 and CHOP in GXD-M and GXD-H groups all visibly descended (P < 0. 01). Conclusions GXD can reduce the excessive apoptosis of IECs and protect intestinal epithelial cell barrier homeostasis by inhibiting the signal transduction of PERK-elF2α-CH0P apoptotic pathway.

Operacionalización psicodinámica del eje estructura en un paciente drogodependiente / Psycho-dynamic Operationalization of the Structure Axis in Drug Addicted Patients / Operacionalização psicodinâmica do eixo estrutura num paciente drogo-dependente

Resumen Objetivo. Comprender la relación entre el perfil psicodinámico y la adicción a las drogas en pacientes internos en una comunidad terapéutica, considerando la operacionalización del eje estructura según el Diagnóstico Psicodinámico Operacionalizado (OPD-2). Método. Se implementó el estudio cualitativo de cuatro procesos individuales de psicoterapia psicodinámica. Cada caso fue analizado por separado y se operacionalizaron los ejes diagnósticos del OPD-2; instrumento validado internacionalmente para la investigación, evaluación, focalización e indicación del tratamiento. Resultados. El análisis de caso permitió concluir que las limitaciones en las capacidades cognitivas, emocionales y vinculares corresponden a un nivel estructural medio-bajo. Discusión. Para la intervención psicoterapéutica es importante comprender la dinámica psíquica que sostiene la conducta adictiva con base en un acertado diagnóstico dimensional.

Abstract Objective. To understand the relation between the psychodynamic profile and drug addiction in patients hospitalized in a therapeutic community, taking into account the operationalization of the structure axis based on the OperationalizationPsychodynamic Diagnostic (OPD-2). Method. A multi-case method was implemented consisting of the qualitative study of four individual processes of psychodynamic psychotherapy. Each case was analyzed individually and the diagnostic axes proposed by the OPD-2 were operationalized, this being an instrument of psychodynamic orientation, validated internationally through research, evaluation, focalization and treatment indication. The case of a drug-addicted patient in the process of rehabilitation was chosen for the purpose of multi-axial evaluation. Results. The case analysis concluded that limitations in cognitive, emotional and bonding abilities correspond to a middle-low structural level. Discussion. In psychotherapeutic interventions, it is important to understand the psychological dynamic present in addictive behavior starting with an appropriate dimensional diagnosis.

Resumo Escopo . Compreender a relação entre o perfil psicodinâmico e a adição às drogas em pacientes internos numa comunidade terapêutica, considerando a operacionalização do eixo estrutura com base no Diagnóstico Psicodinâmico Operacionalizado (OPD-2). Metodologia . Foi implementado um método multicaso, que consiste no estudo qualitativo de quatro processos individuais de psicoterapia psicodinâmica. Cada um dos casos foi analisado por separado e foram operacionalizados os eixos propostos pelo OPD-2, que é um instrumento de orientação psicodinâmica validado internacionalmente para a pesquisa, avalição, focalização e indicação do tratamento. Para o propósito da avaliação multiaxial, foi elegido o caso de um paciente drogo-dependente em processo de reabilitação. Resultados . A análise de caso permitiu concluir que as limitações nas capacidades cognitivas, emocionais e vinculares correspondem a um nível estrutural médio-baixo. Discussão . Para a intervenção psicoterapêutica é importante compreender a dinâmica psíquica que sustenta o comportamento aditivo com base num acertado diagnóstico dimensional.

Expression and correlation of SIRT1 and inflammatory factors in peripheral blood of patients with major depressive disorder / 中华行为医学与脑科学杂志

Objective To explore the expression changes of SIRT1 and related inflammatory regula-tors in peripheral blood of patients with major depressive disorder and analyze the correlation between SIRT1, depression and inflammatory regulators. Methods Forty patients with major depressive disorder and forty healthy controls were selected. Hamilton Depression Scale (HAMD-17) was used to assess the degree of de- pression in patients with depressive disorder. Quantitative Real-time PCR( RT-PCR) was used to detect the relative expression levels of SIRT1,Elf-1,NF-κB,IL-1β,GM-CSF mRNA,and enzyme linked immunosorbent assay(ELISA) was used to detect the expression levels of SIRT1,Elf-1,NF-κB,IL-1β,GM-CSF proteins. The correlation between the severity of depression disorder and SIRT1 and the correlation between SIRT1 and Elf-1 and NF-κB were analyzed. Results (1)Compared with the control group,SIRT1 mRNA expression significantly decreased in the case group (P<0. 01),while Elf-1,NF-κB,IL-1β,GM-CSF mRNA expression significantly increased in the case group (P<0. 01). ( 2) The expression of plasma SIRT1 protein((8. 23± 1. 78)ng/ml) in the case group was lower than that in the control group (P<0. 01). The expressions of plas-ma Elf-1 protein((1 921. 67±271. 07)pg/ml),NF-κB protein((2 057. 29±260. 44)pg/ml),IL-1β protein ((186. 60±31. 00) pg/ml) and GM-CSF protein((183. 69±28. 87) pg/ml) were higher than those in the control group((1 512. 92±284. 54)pg/ml,(1537. 18±313. 82) pg/ml,(144. 79±31. 48) pg/ml,(162. 82± 27. 90) pg/ml,respectively,all P<0. 01). (3) SIRT1 mRNA expression level was negatively correlated with the severity of major depressive disorder (r=-0. 51, P<0. 01) and was negatively correlated with the mRNA expression levels of Elf-1 and NF-κB (r=-0. 66,P<0. 01,r=-0. 64,P<0. 01). Conclusion The expres-sion level of SIRT1 in peripheral blood of patients with major depressive disorder is correlated with the sever-ity of depression. This may be related to the decrease of SIRT1 expression in peripheral blood leukocytes of patients with major depressive disorder,which activates the pathway of NF-κB and Elf-1 and increase expres-sion of GM-CSF and IL-1β.

Usefulness of Enhanced Liver Fibrosis, Glycosylation Isomer of Mac-2 Binding Protein, Galectin-3, and Soluble Suppression of Tumorigenicity 2 for Assessing Liver Fibrosis in Chronic Liver Diseases

BACKGROUND: Liver biopsies have been partially replaced by noninvasive methods for assessing liver fibrosis. We explored the usefulness of four novel biomarkers, enhanced liver fibrosis (ELF), glycosylation isomer of Mac-2 binding protein (M2BPGi), galectin-3, and soluble suppression of tumorigenicity 2 (sST2), in association with liver fibrosis. METHODS: ELF, M2BPGi, galectin-3, and sST2 were assayed in 173 patients with chronic liver diseases. The results were analyzed according to fibrosis grade (F0/1, F2, and F3/4) by transient elastography (TE). RESULTS: ELF, M2BPGi, galectin-3, and sST2 values differed significantly according to TE grade; ELF and M2BPGi values were higher in F2 and F3/4 than in F0/1 (P≤0.001, all), sST2 values were higher in F3/4 than in F0/1 and F2 (P < 0.05), and galectin-3 values were higher in F3/4 than in F0/1 (P=0.0036). ELF and M2BPGi showed good TE fibrosis detection performance (area under the curves [AUC], 0.841 and 0.833 for ≥F2; and 0.837 and 0.808 for ≥F3). The sensitivity and specificity for predicting TE grade F≥2 were 84.1% and 76.7% for ELF and 63.6% and 91.5% for M2BPGi. CONCLUSIONS: This is the first study to compare the liver fibrosis assessment of four novel biomarkers: ELF, M2BPGi, galectin-3, and sST2. The biomarkers varied significantly according to TE grade, and each biomarker showed a different trend. ELF and M2BPGi seem to have comparable good performance for detecting liver fibrosis.

Survey Of Emf Emitted From Air Conditioners And Switchboards In Electrical And Electronic Engineering Laboratories of Southeast University Bangladesh

The aim of this survey is to investigate whether the Electromagnetic Fields (EMF) emitted from various Air conditioners and Electrical Switch boards at different Labs at the Electrical and Electronic Engineering (EEE) department of Southeast University, Bangladesh,affects the students. There is a standard threshold value recommended by World Health Organization (WHO) for both electric and magnetic fields. Electro-Magnetic Fields commonly known as Non Ionizing Radiation is emitted from high power transmission lines, computer monitor/video display unit, radio waves of different frequencies (extremely low frequency to microwaves), telecommunication, satellite, radar etc. which causes health hazards to living system and environment. There has been no such study performed in Bangladesh. Magnetic Fields around the equipments as well as the threshold values of Electric and Magnetic fields were measured.

The clinical and genetic features of a case with antenatal form of leukoencephalopathy with vanishing white matter disease / 临床儿科杂志

Objective To explore the clinical features and gene mutations of antenatal form leukoencephalopathy with vanishing white matter disease (VWM).Methods The clinical data and genetic test results in a patient with antenatal form of VWM were retrospectively analyzed.Results A three months old patient was admitted to our hospital with intermittent convulsions commenced from the first month after birth.The baby had low birth weight (1900g) and asphyxia at birth.Developmental retardation and cataracts in both eyes were found on physical examination,and the patient couldn't stare,gaze-following,be amused and raise his head.In addition,he showed hypermyotonia of both lower extremities.Diffused and symmetrical abnormal signals same as that of the cerebrospinal fluid in the cerebral white matter were observed by brain CT and MRI scanning,and the lesions were gradually enlarged.Moreover,a missense mutation (c.1016G＞A) and a frameshift mutation (c.1809delC) in EIF2B5 gene inherited from his parent were detected by DNA sequencing.Conclusions VWM is one of the most prevalently inherited childhood white matter disorders,but the case of antenatal form is very rare.The diagnosis should be based on clinical manifestations and EIF2B genetic analysis.To our knowledge,the frameshift mutation c.1809delC has never been reported to date.

ELF (Enhanced Liver Fibrosis) como marcador não invasivo de fibrose hepática na hepatite C crônica / ELF (Enhanced Liver Fibrosis) as a non invasive predictor of liver fibrosis in hepatitis C

A fibrose hepática é o aspecto mais relevante e o mais importante determinante de morbimortalidade na hepatite C crônica (HCC). Historicamente, a biópsia hepática é o método de referência para avaliação da fibrose causada pela HCC, apesar de apresentar limitações. O estudo de marcadores não invasivos, que possam obviar a necessidade da biópsia, é uma área de constante interesse na hepatologia. Idealmente, a avaliação da fibrose hepática deveria ser acurada, simples, prontamente disponível, de baixo custo e informar sobre o prognóstico da patologia. Os marcadores não invasivos mais estudados são a elastografia hepática transitória (EHT) e os laboratoriais. A EHT já foi extensamente validada na HCC e está inserida na rotina de avaliação destes pacientes. Dentre os laboratoriais, existem diversos testes em continua experimentação e, até o momento, nenhum foi integrado à prática clínica no Brasil, embora já aplicados rotineiramente em outros países. O Enhanced Liver Fibrosis (ELF), um teste que dosa no soro ácido hialurônico, pró-peptídeo amino-terminal do colágeno tipo III e inibidor tissular da metaloproteinase 1, tem se mostrado bastante eficaz na detecção de fibrose hepática significativa e de cirrose na HCC. Neste estudo o ELF teve o seu desempenho avaliado em relação a biópsia hepática e demonstrou apresentar boa acurácia na detecção tanto de fibrose significativa quanto de cirrose. Na comparação com a EHT apresentou acurácia semelhante para estes mesmos desfechos, com significância estatística. No entanto, foi observada uma superestimação da fibrose com a utilização dos pontos de corte propostos pelo fabricante. Este achado está em acordo com a literatura, onde não há consenso sobre o melhor ponto de corte a ser empregado na prática clínica. Com a ampliação da casuística foi possível propor novos pontos de corte, através da análise clássica, com a biópsia hepática como padrão ouro...

Liver fibrosis is the most relevant issue concerning chronic hepatitis C (CHC) and determines its prognosis. Historically, liver biopsy has been the reference method for evaluating fibrosis related to CHC, though it presents many drawbacks. There is a continuing interest in the development of non invasive markers capable of replacing liver biopsy. The ideal surrogate for fibrosis evaluation should be accurate, simple, low cost and yield prognostic information. So far, the most well known non invasive methods are transient hepatic elastography (TE) and laboratory panels. TE has already been extensively validated and is integrated in patients routine. There is plenty of laboratory panels in continuing evaluation and some are already integrated in daily practice abroad. In Brasil, until the present moment, it is not a reality. Enhanced Liver Fibrosis (ELF) panel comprises the serum concentration of hyaluronic acid, tissue inhibitor of matrix metalloproteinases-1, and aminoterminal propeptide of type III procollagen and has demonstrated good performance in detecting significant fibrosis and cirrhosis in CHC patients. In the present study ELF had it’s performance evaluated against liver biopsy and obtained satisfactory accuracy in detecting significant fibrosis and cirrhosis. In comparison to TE no statistically significant diference was observed, for the same endpoints mentioned before. However, the application of manufacturer’s cutoff points produced overestimation of fibrosis stages. These findings are in accordance with other author’s results, in that there is no consensus so far on the most adequate cutoff points for main clinical end points. Enlarging the data permited calculating new cutoff points, through the classical statistical approach, using liver biopsy as the gold standard...

Caracterización del ambiente físico en viviendas de hormigón en "La Coronela", La Habana, 2010-2011 / Characterization of physical environment in concrete houses, "La Coronela", Havana, 2010-2011

Introducción: el creciente aumento de la población ha ocasionado un déficit cuantitativo de viviendas, el cual es abordado desde diferentes soluciones constructivas. Una de ellas es la vivienda de edificios multifamiliares de hormigón, conocida como FORSA. Objetivos: caracterizar, desde el punto de vista sanitario, ambientes físicos de viviendas FORSA de edificios multifamiliares en el asentamiento "La Coronela", en La Habana. Métodos: Para lograr esta caracterización se estudiaron factores de riesgo, como el microclima, temperaturas de cubiertas y envolventes y la penetración del viento, campos de radiación no ionizante de muy baja frecuencia (ELF), clima luminoso y ruido durante una semana correspondiente a la estación húmeda del año 2010 y otra a la estación seca de 2011. Se seleccionaron nueve viviendas de edificios multifamiliares de tres y cinco plantas, ubicadas en niveles bajos y altos, con y sin aleros, y con distintas orientaciones de fachada. Resultados: existía calor moderado en las dos estaciones estudiadas, que fue algo más cálido en las viviendas de edificios multifamiliares de cinco plantas. El nivel de la vivienda y la presencia de aleros no parecieron ofrecer efecto importante en el clima interior. El viento exterior penetró poco en los interiores. El componente magnético del campo ELF no transgredió los valores guía de la Organización Mundial de la Salud. La iluminación natural resultó apropiada, no así la artificial, pero los coeficientes de reflexión de pisos resultan elevados. El nivel sonoro incumplió la norma sanitaria vigente NC 26 de 2012 aplicada para nuevas urbanizaciones. Conclusiones: El ambiente interior de las viviendas FORSA del asentamiento "La Coronela" presenta un clima ligeramente inconfortable para los residentes, independientemente del nivel de la vivienda y la presencia de aleros.

Background: The growing increase in population has caused a quantitative deficit of houses which has been approached from different constructive solutions. One of these is the house of multifamily buildings made of concrete, known as FORSA. Objectives: to characterize, from a sanitary point of view, physical environments of FORSA houses of multifamily buildings in "La Coronela" settlement in Havana. Methods: to achieve this characterization, different risk factors were studied such as microclimate, cover and enclosure temperatures and wind penetration, non-ionizing radiation fields of extremely low frequency (ELF), light climate and noise during a week corresponding to the wet season of the year 2010 and another corresponding to the dry season of the year 2011. Nine houses of multifamily buildings of three and five floors were selected. They were located in low and high levels, with or without eaves and with different facade orientations. Results: there was moderate warmth in the two seasons studied, which was somewhat warmer in the houses of five floor multifamily buildings. The level of the houses and the presence of eaves seemed to offer no significant effect on the indoor climate. The external wind hardly penetrated indoors. The magnetic component of ELF field did not transgress the reference values of the World Health Organization. Natural lighting was appropriate but not the artificial one; whereas the reflection coefficients of floors were higher. The sound level breached the current sanitary standard NC 26 of 2012. Conclusions: the environment inside FORSA houses in the settlement "La Coronela" presents a slightly uncomfortable climate for residents, regardless the levels of houses and the presence of eaves.

Factors Associated with Self-rated Health in the Rural Population: Age- and Gender-specific Analysis / Journal of Rural Medicine

<b>Objective:</b> Multiple studies worldwide have supported the predictive validity of self-rated health (SRH) with regard to disability and mortality among elderly people. Although SRH is an important study topic providing clues to enhance a person’s quality of life, there is currently insufficient data on age- and gender-specific differences among factors associated with SRH in Japan, particularly in rural areas. The present study examined the factors associated with SRH of a segment of Japan’s rural population by age- and gender-specific analysis.<br><b>Methods:</b> We used data from a cohort study of all users who underwent an annual health checkup at a public clinic in a rural area. The study subjects were 155 male and 169 female users from June 2009 to August 2010 who agreed to participate in this study. We divided the study subjects into 4 categories as follows: men aged less than 65, women aged less than 65, men aged 65 and over, and women aged 65 and over. The subjects who responded positively to the SRH-related questions were defined as the high SRH group, and those who responded negatively were defined as the low SRH group. We then compared the data between the high and the low groups in each category.<br><b>Results:</b> In all four categories, there were statistically significant differences in regular hospital or clinic attendance between the high and low SRH groups. In all four categories, there were no significant differences in eating or exercise habits between the two SRH groups.<br><b>Conclusion:</b> Because regular hospital or clinic attendance by a subject is indicative of the presence of chronic health problems, it is natural for the subject’s perception of their own health to be negative. However, rural physicians should provide patients with emotional and psychological support to deal with any health-related concerns positively.

Influence of Exposure to Extremely Low Frequency Magnetic Field on Neuroendocrine Cells and Hormones in Stomach of Rats

Extremely low frequency magnetic fields (ELF-MF) have the ability to produce a variety of behavioral and physiological changes in animals. The stomach, as the most sensitive part of the neuroendocrine organ of the gastrointestinal tract, is crucial for the initiation of a full stress response against all harmful stress. Thus, the purpose of this study was to examine whether ELF-MF stimuli induce changes in the activity of neuroendocrine cells, considering their involvement in endocrine or paracrine effect on surrounding cells. The exposure to ELF-MF (durations of 24 h and 1 or 2 weeks, 60 Hz frequency, 0.1 mT intensity) altered the distribution and occurrence of gastrin, ghrelin and somatostatin-positive endocrine cells in the stomach of rats. The change, however, in the secretion of those hormones into blood from endocrine cells did not appear significantly with ELF-MF exposure. Comparing with sham control, ELF-MF exposure for 1 and 2 week induced an increase in BaSO4 suspension propelling ratio of gastrointestinal tract, indicating that ELF-MF affects gastrointestinal motility. Our study revealed that ELF-MF exposure might influence the activity of endocrine cells, an important element of the intrinsic regulatory system in the digestive tract. The pathophysiological character of these changes and the mechanism responsible for neuroendocrine cell are still unclear and require further studies.

Defectos en la síntesis de cadena ζ en el linfocito T, generando señales intracelulares equivocadas, responsables de lupus eritematoso sistémico / Defects in the synthesis of ζ chain in T lymphocytes, generating wrong intracellular signals, responsible for systemic lupus erythematosus

La expresión anormal de moléculas claves en señalización y la función defectuosa de los linfocitos T cumplen un papel significativo en la patogénesis de la enfermedad autoinmune. Las células T muestran numerosas anormalidades en la señalización del complejo TCRζ¹, estas aberraciones resultan en la alteración de la expresión de citoquinas. Mientras algunas de estas anormalidades explican el aumento de la actividad de células B por células T con incremento de los anticuerpos, la disminución en la producción de IL-2 resulta en un aumento en la susceptibilidad a las infecciones, reducción en la activación de las células T, inducción de la muerte celular y prolongada sobrevida de las células T autorreactivas².

The abnormal expression of key molecules in signaling and the malfunction of the T cell T have a significant activity in the pathogenesis of the autoimmune disease. The cells T exhibit numerous abnormalities in the signaling of the complex TCRζ¹, these aberrations result in the alteration of the citoquines. While some of these abnormalities explain the increase of the activity of cells B for cells T with increment of the antibodies production, the decrease in the production of IL-2 induces an increase in the susceptibility to the infections, diminishing in the activation of the cells T, and expansion of the lifespan of the autorreactive cells².

Expression of T cell-specific transcriptional factor Elf-1 in mononu-clear cells, CD4~+ T cells and CD8~+ T cells from umbilical cord blood / 中国病理生理杂志

AIM: To investigate the expression level of transcriptional factor Elf-1 in mononuclear cells, CD4~+ and CD8~+ T cells from umbilical cord blood (UCB). METHODS: Real-time PCR with SYBR green I technique was used for detecting the Elf-1 expression in mononuclear cells, sorted CD4~+ and CD8~+ T cells from 12 cases of umbilical cord blood. The relative mRNA expression level of Elf-1 was analyzed by a formula of 2~(-△Ct)×100%. The expression level of β_2-microglobulin gene (β_2M) was used as an endogenous reference. The peripheral blood from 10 cases of healthy adults was served as control. RESULTS: Elf-1 mRNA expressed in all blood samples collected from both UCB and healthy adults. The expression level showed apparent diversity in different individuals. The relative mRNA expression of Elf-1 in both mononuclear cells (18.55%±2.48%) and CD8~+ T cells (3.52%±0.45%) from UCB were significantly higher than those from healthy adults (9.16%±1.92%, 2.02%±0.27%, respectively, P<0.01, P<0.05). CONCLUSION: The results of Elf-1 expression level from umbilical cord blood indicate that the over-expression of Elf-1 gene in mononuclear cells and CD8~+ T cells might be one of the features of T cell immune state in umbilical cord blood.

The progress of the special transcription factor of T lymphocytes,Elf-1 / 白血病·淋巴瘤

Elf-1 (E74-like factor 1) is a member of Ets transcription factor family,which participated in physiological and pathological process of cells proliferation,differentiation and tumorigenesis. In this review,we summarize that Elf-1 is related to T cell differentiation and development,tumor and autoimmune disease.

Effect of alternating the magnetic feld on phosphate metabolism in the nervous system of Helix pomatia

The effect of extremely low frequency magnetic felds (50 Hz, 0.5 mT) - ELF-MF, on phosphate metabolism has been studied in the isolated ganglions of the garden snail Helix pomatia, after 7 and 16 days of snail exposure to ELF-MF. The infuence of ELF-MF on the level of phosphate compounds and intracellular pH was monitored by 31P NMR spectroscopy. Furthermore, the activity of enzymes involved in phosphate turnover, total ATPases, Na+/K+-ATPase and acid phosphatase has been measured. The exposure of snails to the ELF-MF for the period of 7 days shifted intracellular pH toward more alkaline conditions, and increased the activity of investigated enzymes. Prolonged exposure to the ELF-MF for the period of 16 days caused a decrease of PCr and ATP levels and decreased enzyme activity, compared to the 7-day treatment group. Our results can be explained in terms of: 1. increase in phosphate turnover by exposure to the ELF-MF for the period of 7 days, and 2. adaptation of phosphate metabolism in the nervous system of snails to prolonged ELF-MF exposure.

Effects of extremely low frequency magnetic field on growth kinetics, metabolism and cell cycle of human liver cancer cells / 现代生物医学进展

Objective: To study the effects of extremely low frequency (ELF) magnetic field with fixed parameters on human liver cancer cells (SK-HEP-1) at different aspects. Methods: SK-HEP-1 cells were exposed to 50Hz, 20mT magnetic field during the whole culture process, and then proliferation activity, growth kinetics, metabolic profile and cell cycle were analyzed. Results: 50Hz, 20mT magnetic field inhibits the growth and metabolism of SK-HEP-1 cells, and hampers their mitotic division. Conclusion: 50Hz, 20mT magnetic field could be a potential therapy in the treatment of human malignant tumors.

ENZYME LABELED FLUORESCENCE 97 TO DETECT THE ACTIVITY OF ALKALINE PHOSPHATASE IN NEUTROPHILS / 解剖学报

Objective To localize endogenous alkaline phesphatase in human neutrophils with ELF(enzyme-labeled fluorescence)-97 phosphate,which yields an intensely fluorescent yellow-green precipitate at the site of enzymatic activity. Methods Neutrophils were isolated from human blood and fixed,then histochemistry with the ELF-97 phosphate was performed with the ELF-97 endogenous phosphatase detection kit.All photography was performed with a Nikon labophot fluorescence/DIC microscope. Results Fluorescent yellow-green granules,well-distributed but size-varied,were observed in neutrophils under fluorescence microscope.There were 94.102?3.133 percent rate of positive neutrophil alkaline phosphatase among 51 cases of healthy volunteers.Conclusion ELF-97 endogenous phosphatase detection kit can provide sensitive,high-resolution localization of endogenous phosphatase activity in neutrophils.

Is Epidemiology Implicating Extremely Low Frequency Electric and Magnetic Fields in Childhood Leukemia?

We have reviewed epidemiological studies examining the association between residential exposure to extremely low frequency electric and magnetic fields (ELF-EMF) and childhood leukemia. We have excluded studies focusing on electrical appliances, because it is difficult to consolidate transient exposure from multiple sources and equally difficult to control information bias. We have identified 24 studies of residential exposure to ELF-EMF and childhood leukemia. About half of these studies were reported as positive and the remaining as null. For each of the studies reported as positive, however, one or more sources of bias could not be confidently excluded. Moreover, studies which were methodologically more sound, or benefited from high quality registry data, were more frequently null than other investigations. We conclude that the empirical evidence in support of an association between ELF-EMF and childhood leukemia is weak.

Is epidemiology implicating extremely low frequency electric and magnetic fields in childhood leukemia?

We have reviewed epidemiological studies examining the association between residential exposure to extremely low frequency electric and magnetic fields (ELF-EMF) and childhood leukemia. We have excluded studies focusing on electrical appliances, because it is difficult to consolidate transient exposure from multiple sources and equally difficult to control information bias. We have identified 24 studies of residential exposure to ELF-EMF and childhood leukemia. About half of these studies were reported as positive and the remaining as null. For each of the studies reported as positive, however, one or more sources of bias could not be confidently excluded. Moreover, studies which were methodologically more sound, or benefited from high quality registry data, were more frequently null than other investigations. We conclude that the empirical evidence in support of an association between ELF-EMF and childhood leukemia is weak.

Expression of T cell-specific transcriptional factor Elf-1 in mononu-clear cells,CD4~+ T cells and CD8~+ T cells from umbilical cord blood / 中国病理生理杂志

AIM:To investigate the expression level of transcriptional factor Elf-1 in mononuclear cells,CD4+ and CD8+ T cells from umbilical cord blood (UCB). METHODS: Real-time PCR with SYBR green I technique was used for detecting the Elf-1 expression in mononuclear cells,sorted CD4+ and CD8+ T cells from 12 cases of umbilical cord blood. The relative mRNA expression level of Elf-1 was analyzed by a formula of 2-△Ct?100%. The expression level of ?2-microglobulin gene (?2M) was used as an endogenous reference. The peripheral blood from 10 cases of healthy adults was served as control. RESULTS: Elf-1 mRNA expressed in all blood samples collected from both UCB and healthy adults. The expression level showed apparent diversity in different individuals. The relative mRNA expression of Elf-1 in both mononuclear cells (18.55%?2.48%) and CD8+T cells (3.52%?0.45%) from UCB were significantly higher than those from healthy adults (9.16%?1.92%,2.02%?0.27%,respectively,P