RESUMO
Objective To study the effective constituents from the stem bark of Erythrina variegata.Methods The compounds Ⅰ-Ⅸ were isolated by a combination of silica gel,Sephadex LH-20,and ODS chromatographies and their structures were identified by spectral methods.Results Nine known compounds were isolated from the stem bark of E.variegata.Their structures were identified by means of spectroscopic analysis as: 5,4′-dihydroxy-8-(3,3-dimethylally)-2″,2″-dimethylpyrano [5,6∶6,7] isoflavone(Ⅰ),stigmasterol(Ⅱ), erythrinasinate B(Ⅲ),3-hydroxy-2′,2′-dimethylpyrano [5,6∶9,10] pterocarpan(Ⅳ),5,7-dihydroxy-8-(3,3-dimethylally)-dihydroflavanone (Ⅴ),5,4′-dihydroxy-2″,2″-dimethylpyrano [5,6∶6,7] isoflavone(Ⅵ),5,7,4′-trihydroxy-6,8-di(3,3-dimethylally) isoflavone(Ⅶ),5,2′,4′-trihydroxy-8-(3,3-dimethylally)-2″,2″-dimethylpyrano [5,6∶6,7] isoflavone(Ⅷ),5,4′-dihydroxy-6-(3,3-dimethylally)-2″,2″-dimethylpyrano [5,6∶7,8] isoflavone(Ⅸ).Conclusion Compounds Ⅰ,Ⅲ,Ⅳ,Ⅶ,and Ⅸ are isolated from this plant for the first time and compound Ⅴ is newly isolated from the plant of Erythrina Linn.
RESUMO
Human erythrocyte specific lectin was isolated from the seeds of Erythrina variegata Linn. var. orientalis Linn. Merrill. The lectin preferentially agglutinated erythrocytes in the sequence of O>B>A = AB. The lectin was purified 19-fold by affinity chromatography on acid treated sepharose 4B with an yield of 81%. The purified lectin was found homogeneous on polyacrylamide gel electrophoresis. The erythroagglutination reaction was inhibited by N-acetyl-D-galactosamine, D-galactose and lactose at very low concentration. The haemagglutination by the purified lectin was not inhibited by different hexose and pentose sugars even at high concentration. The purified lectin was a glycoprotein and agglutinated leucocytes at 3 μg protein concentration. The lectin induced transformation of peripheral blood lymphocytes in cultures