RESUMO
Objective:To improve the HPLC method for the determination of esmolol hydrochloride injection .Methods: HPLC was used and the column was a Welch Xltimate C 18 column(250 mm ×4.6 mm, 5μm).The mobile phase was acetonitrile -methanol-phosphate buffer (15:20:65), and the detection wavelength was 222 nm.The flow rate was 1.0 ml· min-1, the column temperature was 30℃, and the sample volume was 20μl.Results:The concentration of esmolol hydrochloride showed a good linear relationship ( r=1.0000) with the peak area within the range of 12.26-196.16 μg· ml-1, and the average recovery was 101.2%(RSD=0.7%, n=9).Conclusion:The method is simple, rapid, accurate and reproducible , which can show truer drug content when compared with the current standard , and is more suitable for the content determination of esmolol hydrochloride injection .