RESUMO
The genus Flemingia Roxb. ex W. T. Ait. (Leguminosae) was originally recorded in "Zhiwu Mingshi Tukao", written by WU Qi-jun in Qing Dynasty. Its abundant sources were widely used for multi-nationalities in a long history of application. This review mainly focused on the general applied situations, chemical constituents and pharmacological effects, thus provided a basis for further investigation of genus Flemingia Roxb. ex W.T. Ait., and offered a better understanding of its developing market potential. So far, 197 compounds have been separated and identified from genus Flemingia Roxb. ex W.T. Ait., including flavonoids, saponins, anthraquinones, triterpenes, volatile oil, etc. Moreover, modern pharmacological studies have shown that several pharmacological effects have been found from the extracts and individual compounds (mainly flavonoids), including analgesia and anti-inflammatory, antibacterial and antifungal, estrogen, skin protection, neuroprotection, hepatoprotective effect, and so on. The genus Flemingia Roxb. ex W.T. Ait. is rich in resources and promiscuous in germplasm resources, which results in the remarkble differences in regions, time as well as the different parts of the content chemical composition,and there is no standardized control index of quality. With the development of modern separation and identify techniques, the determination of multiple chemical components has been widely accepted for quality control of genus Flemingia Roxb. ex W.T. Ait. On the whole, these achievements will further expand the existing market potential of the plants in the genus and provide a beneficial support to its further clinical use in modern medicine.
RESUMO
Objective: To screen a DNA barcoding sequence that can identify the medicinal plants of Flemingia Roxb. ex Ait. et Ait. f. accurately and efficiently. Methods: Four species and 14 individuals of Flemingia Roxb. ex Ait. et Ait. f. were collected, the (second internal transcribed spacer, ITS2) of ribosomal DNA, rbcL, psbA-trnH of chloroplast DNA were amplified and sequenced. Meanwhile, the NCBI data were retrieved and the according sequence was downloaded. The total numbers of species and individuals were six and twenty. The PCR amplification and sequencing efficiency, barcoding gap, and NJ trees were used to evaluate the efficiency of species identification. Results: The sequencing success rates of ITS2, rbcL, and psbA-trnH were 100%, 100%, and 85.71%, respectively; Among the three DNA barcoding sequences, only ITS2 has remarkable barcoding gap; ITS2 could distinguish every species of Flemingia Roxb. ex Ait. et Ait. f. (except F. philippinensis and F. stricta). Conclusion: ITS2 could identify the medicinal plants of Flemingia Roxb. ex Ait. et Ait. f. accurately and efficiently, and could be used as an ideal DNA barcoding of species identification for medicinal materials of Flemingia Roxb. ex Ait. et Ait. f.
RESUMO
Objective: To analyze the genetic relationship of the plants in Flemingia Roxb. ex Ait. et Ait. f. and make preliminary quality accessment, in order to provide the theoretical basis for screening the new medicinal resource. Methods: Inter-simple sequence repeats (ISSR) markers were used to analyze the genetic relationship of 14 species in Flemingia Roxb. ex Ait. et Ait. f.. Meanwhile, spectrophotometry was used to measure total flavones. Results: Thirty primers were screened out of 60 ISSR random primers and produced 367 bands totally. Among them 363 bands (98.91%) were polymorphic, four bands were owned by all; The genetic similarity (GS) was 0.5668-0.8338, showing abundant genetic diversity; F. wallichii and F. philippinensis have the closest genetic relationship, F. lineate and F. chappar have the largest genetic distance; The highest content of total flavones is in F. mengpengensis and secondary is in F. philippinensis, total content of flavones in F. wallichii is above average. Conclusion: The plants in Flemingia Roxb. ex Ait. et Ait. f. have the abundant genetic diversity. As medicinal plants, F. wallichii has the potential for replacing F. philippinensis.