Advances on the treatment of Fusobacterium nucleatum-promoted colorectal cancers using nanomaterials / 生物工程学报

Fusobacterium nucleatum (Fn) is an oral anaerobic bacterium that has recently been found to colonize on the surface of colorectal cancer cells in humans, and its degree of enrichment is highly negatively correlated with the prognosis of tumor treatment. Numerous studies have shown that Fn is involved in the occurrence and development of colorectal cancer (CRC), and Fn interacts with multiple components in the tumor microenvironment to increase tumor resistance. In recent years, researchers have begun using nanomedicine to inhibit Fn's proliferation at the tumor site or directly target Fn to treat CRC. This review summarizes the mechanism of Fn in promoting CRC and the latest research progress on Fn-related CRC therapy using different nanomaterials. Finally, the applications perspective of nanomaterials in Fn-promoted CRC therapy was prospected.

The inhibitory effect of ozone water on bad breath pathogens in vitro / 实用口腔医学杂志

Objective: To study the inhibitory effect of ozone water on bad breath pathogens in vitro. Methods: In vitro cultured bad breath pathogens Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi) and Fusobacterium nucleatum (Fn) were identified by Gram stain and a PCR test. Ozonated water by was prepared by ozone generator and the concentration of ozone in water was measured using iodine titration. Artificial saliva was used to observe its interference on ozone function. Results: Gram stain and PCR results were consisted with strain characteristics. The ozone concentration of ozonated water reached to the maximum of 0. 2 mg/L. Ozone water with the concentration was 0. 05, 0. 1 and 0. 2 mg/L inhibited the proliferation of Pg, Pi and Fn. But the inhibitory effect was weakened when the concentration decreased. The artificial saliva reduced the effect of ozone water. Conclusion: The Pg, Pi and Fn can be inhibited by ozone water. Artificial saliva may reduce the effects of ozone water on the bacteria.

In vitro antibacterial activity of domestic-made water-soluble propolis against oral anaerobic bacteria from the infected root canal / 重庆医科大学学报

Objective:To evaluate bacteriostasis effect of domestic-made water-soluble propolis on prevotella intermedia(Pi),fu-sobacterium nucleatum(Fn)and mixture bacteria of Pi and Fn in vitro.Methods:With agar dilution,minimal bactericidal con-centrations(MBC)of propolis for Fn,Pi and mixture bacteria were determined.Results:MBC of propolis for Fn,Pi or mixture bacteria were 0.025%,0.025%,0.05% respectively.Conclusion:Propolis shows the favourable antibacterial activity against the main anaerobic bacterium in infectious root canal which it has a value of application for disinfecting root canal.