Evaluation of Elemental and Volatile Compounds of Three Selected Plants of Genus Hibiscus

Aims: The present study aims to evaluate the minerals, bioactive compounds of 3 selected Hibiscus Genus i,e. Hibiscus sabdariffa L., Hibiscus cannabinus L., and Hibiscus acetosella Welw. Place and Duration of Study: The selected plants were collected during May to October 2018 from Imphal (24°37’N and 93°39’E) Manipur North Eastern State of India, which lies 2590 feet above sea level, and study were carried out in Genetics Laboratory, Department of Life Sciences, Manipur University. Methodology: The minerals composition and bioactive compounds were evaluated by using Graphite Flame Atomic Absorption Spectrometer (GF-AAS) method and Gas Chromatography-Mass Spectrometry (GCMS), respectively. Results: The elemental analysis shows the presence of Calcium, Magnesium, Iron, Zinc, Copper, Sodium, Potassium, Selenium, Chromium, Cobalt. By using the GC-MS method, the compounds are identified with Retention time (RT) and area percentage. The two compounds are identified for methanol extract and four compounds for chloroform extract of Hibiscus sabdariffa L. For Hibiscus cannabinus L., three compounds are identified for methanol extract and four compounds for chloroform extract and for Hibiscus acetosella Welw. eleven compounds for methanol extract and three compounds for chloroform extract. Conclusion: The selected plants are good source of Sodium, Potassium, Selenium, Chromium, Cobalt, Calcium, Magnesium, Iron, Zinc, Copper and bioactive compounds which had antibacterial, anticancer, antioxidant properties and renal related disorders protection effects. However, it is needed to study the pharmacological activity for further evaluation.

Determination of Residual Palladium in Bendamustine Hydrochloride by GFAAS / 中国药师

Objective:To develop a method for the determination of palladium in bendamustine hydrochloride by GFAAS. Meth-ods:The sample was destroyed by heat, and the content of palladium was determined by GFAAS with the detection wavelength of 247. 6 nm. Results:The absorbance and the content of palladium showed a good linear relationship within the range of 20-60 ng· ml-1(r=0. 998 4). The average recovery of palladium was 102. 9%(RSD=1. 7%, n=9). Conclusion: The method is sensitive and simple, which can be used for the determination of palladium in bendamustine hydrochloride.

Development of Urine CRM for the quality Control of Heavy Matal Determinations / 대한산업의학회지

Urine certified reference material (CRM) has been developed to help clinical labolatories control analytical accuracy. Two levels of freeze-dried urine were prepared. The low level CRM was made from normal urine and the abnormal level CRM was prepared by spiking the normal urine with As, Cd, Cr, Cu, Hg, Ni and Pb. Urine reference materials were analyzed by graphite furnace atomic absorption spectrometry (GFAAS) and inductively coupled plasma mass spectrometry (ICP-MS) . Analyte elements were separated from matrix elements by using ion exchange resin and hydride generation. Isotope dilution method was employed to enhance analytical accuracy. Round robin test results are also presented which were carried out with 5 clinical laboratories.