Expression of Foxp3 +Tregs and PD1 in gastric cancer tissues and their correlation with clinicopathological factors and prognosis / 中国病理生理杂志

AIM:ToinvestigatetheexpressionofFoxp3+regulatoryTcells(Foxp3+Tregs)andprogrammed death receptor 1 (PD1) in gastric cancer tissues and their association with clinicopathological factors and prognosis of the patients.The correlation between the 2 molecules was also analyzed at the same time.METHODS: The tumor sections from 111 gastric cancer patients were stained for Foxp3 and PD1 by the method of immunohistochemistry.The associations of the expression levels of these 2 molecules with clinicopathological factors involved in the disease progression and progno-sis were statistically analyzed .The relationship of their expression was detected.RESULTS:Foxp3 +Tregs and PD1 were expressed in the gastric cancer tissues, and PD1 was expressed in the tumor infiltrating lymphocytes ( TILs) .The expres-sion of Foxp3 and PD1 was correlated with lymph node metastasis, clinicopathological stage and prognosis of gastric cancer patients.The expression of these 2 determinants in the patients with lymph node metastasis and an advanced clinicopatho-logical stage was distinctly higher ( P<0.05 ) .The patients with positive expression of the 2 indexes presented a lower overall survival rate and worse prognosis (P<0.05).A significantly positive correlation between the infiltration of Foxp3 +Tregs and the expression of PD1+TILs was also observed (P<0.01).CONCLUSION:Foxp3 +Tregs and PD1 +TILs co-infiltrate in the gastric cancer tissues, which can be used as biological markers to predict the disease progression and prog-nosis.

Comparison of laparoscopic total gastrectomy and laparotomic total gastrectomy for gastric cancer / Comparação entre a gastrectomia total laparoscópica e a gastrectomia total laparotômica para o câncer gástrico

BACKGROUND: The use of laparoscopy for the treatment of gastric cancer suffered some resistance among surgeons around the world, gaining strength in the past decade. However, its oncological safety and technical feasibility remain controversial. AIM: To describe the results from the clinical and anatomopathological point of view in the comparative evaluation between the surgical videolaparoscopic and laparotomic treatments of total gastrectomy with linphadenectomy at D2, resection R0. METHOD: Retrospective analyses and comparison data from patients submitted to total gastrectomy with D2 linphadenectomy at a sole institution. The data of 111 patients showed that 64 (57,7%) have been submitted to laparotomic gastrectomy and 47 (42,3%) to gastrectomy entirely performed through videolaparoscopy. All variables related to the surgery, post-operative follow-up and anatomopathologic findings have been evaluated. RESULTS: Among the studied variables, videolaparoscopy has shown a shorter surgical time and a more premature period for the introduction of oral and enteral nourishment than the open surgery. As to the amount of dissected limph nodes, there has been a significant difference towards laparotomy with p=0,014, but the average dissected limph nodes in both groups exceed 25 nodes as recommended by the JAGC. Was not found a significant difference between the studied groups as to age, ASA, type of surgery, need for blood transfusion, stage of the disease, Bormann classification, degree of differentiation, damage of the margins, further complications and death. CONCLUSION: The total gastrectomy with D2 lymphadenectomy performed by laparoscopy presented the same benefits known of laparotomy and with the advantages already established of minimally invasive surgery. It was done with less surgical time, less time for re-introduction of the oral and enteral diets and lower hospitalization time compared to laparotomy, without increasing postoperative ...

RACIONAL: A utilização da videolaparoscopia para o tratamento das neoplasia gástricas sofreu certa resistência entre os cirurgiões ao redor do mundo, ganhando força na década passada. Porém, sua segurança oncológica e viabilidade técnica continuam controversas. OBJETIVO: Demonstrar os resultados do ponto de vista clínico e anatomopatológico na avaliação comparativa entre o tratamento cirúrgico videolaparoscópico e laparotômico da gastrectomia total com linfadenectomia a D2, ressecção R0. MÉTODOS: Estudo retrospectivo dos dados dos prontuários dos pacientes submetidos à gastrectomia total com lindadenectomia a D2, em uma única instituição. Dos 111 prontuários, 64 (57,7%) foram submetidos à gastrectomia laparotômica e 47 (42,3%) à gastrectomia totalmente vídeolaparoscópica. Foram avaliadas variáveis relacionadas ao ato cirúrgico, a evolução pós-operatória e a achados anatomopatológicos. RESULTADOS: A técnica videolaparoscópica demonstrou tempo cirúrgico menor e período para re-introdução alimentar oral e enteral mais precoce que a operação laparotômica. Quanto ao número de linfonodos dissecados, houve diferença significante a favor da laparotômica (p=0,014); porém, a média de linfonodos dissecados em ambos os grupos ultrapassou os 25 preconizados pela JAGC. Não foi encontrada diferença significante entre os grupos estudados no que diz respeito à idade, gênero, ASA, tipo de operação, necessidade de hemotransfusão, estadiamento, classificação de Bormann, grau de diferenciação, comprometimento de margens, complicações e óbito. CONCLUSÕES: A gastrectomia total com linfadenectomia a D2 realizada por videolaparoscopia apresenta os mesmos benefícios conhecidos da cirurgia laparotômica e com as vantagens já estabelecidas da cirurgia minimamente invasiva. Ela apresentou menor tempo cirúrgico, menor tempo para a re-introdução das dietas oral e enteral e tempo de alta menor em relação à laparotômica, sem aumentar as complicações pós-operatórias. .

Meta-analysis of Capecitabine-platinum and S-1-platinum in the Treatment of Advanced Gastric Cancer / 中国药师

Objective:To evaluate the efficacy and safety of capecitabine or S-1 combined with oxaliplatin or cisplatin in the treat-ment of advanced gastric cancer. Methods:Six databases including Cochrane Library, Pubmed, Embase, CNKI, VIP and WanFang were retrieved. Randomized controlled trials ( RCT) in the treatment of advanced gastric cancer were involved for the quality assess-ment and Meta-analysis. Results:A total of 14 RCT involving 1 051 patients were included. There were no significant differences in the total effective rate, complete remission rate and partial remission rate between the two groups(P>0. 05). In the safety evaluation, the incidence of hand-foot syndrome was higher in capecitabine-based regimens than that in S-1-based regimens[RR=2. 59,95%CI (1. 61,4. 17),P0. 05). Con-clusion:Capecitabine combined with oxaliplatin or cisplatin has similar efficacy with S-1 combined with oxaliplatin or cisplatin in the treatment of advanced gastric cancer, and the incidence of hand-foot syndrome is higher in capecitabine-based regimens and the inci-dence of stomatitis is higher in S-1-based regimens.

Roles of Dopamine in Proliferation of Gastric-Cancer Cells

PURPOSE: Dopamine is a neurotransmitter, but in the GIT, the roles of dopamine are a regulator of epithelial cell proliferation, an endogenous protective factor, and a regulator of stomach cancer cell proliferation. By using two different gastric-cancer cell lines, we assessed the effects of dopamine and dopamine receptors on the proliferation of human gastric-cancer cells. MATERIALS AND METHODS: To assess the effects of dopamine and dopamine receptors on the proliferation of human gastric-cancer cells, we investigated cell proliferation and the expression of D1, D2L, and D2S receptor in two gastric-cancer cell lines, SNU 601 and KCU-C2. The effects of dopamine and dopamine receptors on the level of the cell proliferation were determined by staining with an A/H/E (acridine orange, hoechst and ethidium bromide) mixture. RESULTS: After dopamine treatment, the cell viability was significantly decreased in SNU 601 cells (P<0.05) where the D2L receptor was absent, but not in KCU-C2 cells. After treatment with raclopride, a D2 receptor antagonist, dopamine-dose-dependent inhibition of cell proliferation was observed in SNU 601 cells (P<0.05). After treatment with SCH 23390, a D1 receptor antagonist, dopamine significantly increased cell proliferation in KCU-C2 cells (P<0.05), but inhibited cell proliferation in SNU 601 cells (no D2L receptor). CONCLUSION: The dopamine signal via the D1 or the D2S receptor inhibited proliferation of gastric-cancer cells, but that via the D2L receptor increased proliferation. These results suggest that the regulatory effects of dopamine in the gastric-cancer cell proliferation may be controlled by using dopamine receptors.

The Significance of Combined Detection of Multi-drug Resistance Gene in Gastric Cancer Prognosis Judgment / 医学研究杂志

Objective To study the significance of combined detection of lung resistance protein(LRP),P-glycoprotein(P-gp),glutathione S-transferace-?(GST-?) and topoisomeraseⅡ(TopoⅡ) in gastric cancer prognosis judgment.Methods One hundred and thirty-six patients with human gastric carcinomas were studied for the expression of LRP,P-gp,GST-?and TopoⅡ by using immunohistochemistry.Results There were significant relationships in the positive expression rate of LRP,P-gp,GST-?between high-Medium differentiated adenocarcinoma and Poorly differentiated adenocarcinoma,Mucinous carcinoma,and Undifferentiated carcinoma(P

Effect of ginkgo biloba exocarp polysaccharides (GBEP) on p53 gene expression and telomerase activity of human gastriccancer SGC-7901 cells / 中国药理学通报

AIM To study the influence of ginkgo biloba exocarp polysaccharides(GBEP) on p53 gene expression and telomerase activity of human gastriccancer SGC 7901 cells in vitro . METHODS The p53 gene expression level of human gastriccancer SGC 7901 cells were measured by immunohistochemistry ABC. The telomerase activity of human gastriccancer SGC 7901 cells were measured by TRAP ELISA. RESULTS GBEP(80～160 mg?L -1 ,48 h) inhibited the expression of mutated type p53 genes and telomerase activity of human gastriccancer SGC 7901 cells. CONCLUSION The action mechanism of GBEP that inhibited the proliferation of SGC 7901 cells may relate to its down regulation effect on the expression of p53 genes and telomerase activity of human gastriccancer SGC 7901 cells.

The Study of Helicobacter Pylori and Gastric Cancer in Animal / 中国医师杂志

Objective To investigate the relationship of Helicobacter pylori(Hp) and gastric cancer in animal.Methods Ninety of two-grade Wistar male rats were randomly divided into experimental group and control group.In 12 weeks after the rats of experimental group were infected with SS1 Hp strain,animal in two groups were then randomly subdivided into blank ,MNNG subgroup and MNNG+NaCl subgroup,15 rats in each subgroup.Animals in the MNNG and MNNG+NaCl subgroups received 100?g/ml of MNNG in the drinking water,and in the MNNG+NaCl still received 10% NaCl 1 5ml,two times a week for ten months.The blank received water plus libitum.All animals were sacrificed in two months after the above treatment.Histopathological features of the glandular stomach were assessed by HE stainning.Results By the end of the study ,87 rats were survival,3 rats died from the mistakes of perfusion of NaCl.Hp was detected in all infected rats.Adenocarcinoma had developed in the antrum of one experimental rat in the MNNG+NaCl subgroup. Adenomatous hyperplasia were observed in two groups,but its incidence in the MNNG and MNNG+NaCl subgroups of the experiment were 40% and 57 14% respectively,higher than that of the control(6 67% and 15 38%)(P0 05).Conclusions We demonstrated that long-term Hp infection could induce the chronic active and atrophic gastritis in animal model,increase the sensitivity of glandular gastric mucosa to carcinogen,and promote the incidence of pre-carcinomatous lesion.

The clinical-pathogical significance and the expression of maspin mRNA and protein in human gastric carcinoma / 重庆医科大学学报

Objective:To investigate the expression of maspin mRNA and protein in gastric cancer and the relationship between clnical pathological parameters. In order to explore their roles in tumorigenesis and progression of gastric cancer.Methods:Immunohistochemistry SP method was used to detemine expression of maspin in gastric adenocarcinoma,signet-ring cell carcinoma and normal gastric mucosa. The reverse transcriptase PCR was carried out to detect the level of maspin mRNA from the above mentioned 30 gastric adenocarcinoma and 20 normal samples. Results:The expression rate of maspin protein was 50% in gastric adenocarcinoma ,46.7% in signet-ring cell carcinoma,and 90% in normal gastric mucosa ,respectively. The relative transcription level of maspin mRNA in normal gastric mucosa was higher than that in gastric adenocarcinoma .Maspin protein expression was significantly related to the depth of invasion and lymph node metastasis of gastric carcinoma,but did not relate to tumor size and TNMstaging. The relative mRNA transcription level of maspin significantly correlated with the lymph node metastasis and the depth of invasion of carcinoma,but did not relate to TNM staging or tumor size. Conclusion:Down-regulated expression of maspin play an important role in gastric tumor. It may have inhibitory effects on invasion and metastasis of gastric tumor. Maspin might be objective indicator for pathobiological behaviors of gastric tumor.

A primary study for the effect of intraoperative regional arterial infusion chemotherapy on gastric cancer / 重庆医科大学学报

Objective:To evaluate the effect and mechanism ofintraoperative regional arterial infusion chemotherapy(RAIC)on gastric cancer. Methods: The expressions of Ki-67 and VEGF,in 30 cases of gastric cancer,before and after the treatment of intraoperative RAIC,were shown by SP immunohistochemical staining. And pathological changes of tumor were detected. Results:After the intraoperative RAIC,the expressions of Ki-67 and VEGF were significantly lower than those before treatmen(tP