RESUMO
Objective: To investigate the mechanism of berberine regulating glycogen metabolism and its effect on glycogen structure. Methods: The db/db mice were used as diabetic mice to investigate the effect of berberine on blood glucose levels in db/db mice. The liver glycogen was extracted for SEC and TEM analysis to investigate the effect of berberine on liver glycogen and the mechanism of its effect. Results: Berberine significantly decreased the fasting blood glucose level and the insulin level in serum of db/db mice, and berberine repaired the damaged glycogen structure. Meanwhile, berberine decreased the expression of GP, GDBE, cAMP in liver tissue and the glucagon level in serum of db/db mice. Conclusion: Berberine regulated the cAMP/GP signaling pathway, improved hepatic glycogen structure in db/db mice, and repaired damaged glycogen structure, which may be one of the mechanisms for regulating hepatic glucose metabolism and improving symptoms of diabetes.