In vitro Evaluation of Antimicrobial and Cytotoxic Activities of Rosmarinus officinalis L. (Lamiaceae) Essential Oil Cultivated from South-West Tunisia.

Rosmarinus officinalis (Lamiaceae), commonly known as rosemary and iklil, is often used by North African populations for the treatment of several inflammatory and infectious diseases. This study aimed to investigate the antimicrobial and cytotoxic properties of essential oil extracted from the seeds and leaves of R. officinalis. Antimicrobial activity assays involved the determination of inhibition zones and the minimum inhibitory concentration with regards to sixteen pathogenic microbial strains, using disc diffusion and minimum inhibitory concentration methods. The oil showed excellent activity against Staphylococcus aureus, followed by Staphylococcus epidermidis and Staphylococcus aureus 25923, with strong inhibition zones of 38.00, 29.40 and 26.00 mm, respectively. Cytotoxicity assays involved the application of an MTT testing method against HeLa cell lines. The results yielded high IC50 value values of up to 26,77 μg/ml. overall, the findings provided strong support for the strong candidacy of this plant for potential future application, particularly in the food and pharmaceutical industries, as a safe and costeffective natural additive to substitute toxic synthetic food additives.

The Effect of Green Tea Extract on Cisplatin in Cervical Cancer Cell Lines / 대한산부인과학회지

OBJECTIVE: Green tea polyphenol (GTP) has been shown to have anti-tumor properties in a wide variety of experimental systems. In this study, we evaluated the effects of GTP on the cytotoxic effects of cisplatin in cultured HeLa and SiHa cells. METHODS: The cell lines from Korean Cell Culture Bank were cultured in a RPMI-1640 medium supplemented with a 10% fetal bovine serum, antibiotics streptomycin and penicillin. GTP was extracted from tea leaves (Camellia scinensis) by water extraction and organic solvent fractionation. Cells were seeded at 1 x 10(4) cells/well in RPMI1640 media in triplicate wells on a Nunc Labware 96 well flat bottom microculture plate, with and without GTP (100 microgram/mL) and at different concentrations of cisplatin (0-1000 microgram/mL). After incubating the plates at 37 degrees C in 5% CO2 for 2 days, cell viability was determined using the MTT [3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; thiazolyl blue] assay. RESULTS: The viability of the HeLa cells was decreased to 14% at a 600 microgram/mL concentration of cisplatin, and to 16% at 600 microgram/mL in the SiHa cells as measured by the MTT assay. However, in the HeLa cell, co-cultured with GTP (100 microgram/mL), the cell viability decreased to 68% at 200 microgram/mL of cisplatin and to 17% at 400 microgram/mL of cisplatin. And in the SiHa cell, co-cultured with GTP (100 microgram/mL), the cell viability decreased to 48% at 200 microgram/mL of cisplatin and to 17% at 400 microgram/mL of cisplatin. CONCLUSION: This study showed that cisplatin with GTP seems to have a potentiating effect on Cisplatin cytotoxicity than cisplatin alone.