Relationship between hepatitis B virus X protein and hepatic cellular cancer / 药学学报

Hepatic cellular cancer (HCC) is one of the most common cancers in the world, which is a serious threat to human health and life quality. More than 700 000 people die of HCC each year on average, and its incidence increases in many countries. Chronic hepatitis B virus (HBV) infection has been identified as a dominant risk factor for HCC. The pathogenesis of HBV-induced hepatocarcinogenesis is, however, incom-pletely understood. Evidence currently available supports a key role of the HBV X protein (HBx) in the cancer transformation and malignant tumor metastasis. HBx is a multifunctional regulator that may cooperate with the host factors to exert its effects on transcription, signal transduction, cell cycle progression, apoptosis, protein degradation, expression of oncogene and anti-oncogene. This review presents the current knowledge in the molecular pathogenesis of HBx in the induction of HCC.

The research of early diagnosis for the rat hepatom model / 重庆医科大学学报

Objective:to research how to implement early diagnosis for the hepatom model when the rats are alive.Methods:40 young male Wistar rats(30～40 d)were randomly divided into two groups,20 rats in each group(the control group and the interruptedly low doses experimental group).The control group was fed with physiologic saline for 20 weeks.The interruptedly low doses experimental group was fed with 0.2%diethylnitrosamine(DENA)at a dose of 10 mg/kg every week 5 times.The experimental group was induced for 15 continuous weeks.All of them were sacrificed at the 20th week when the rats were inspected by MRI and weighed.The histological changes of liver tissue were observed under microscope.The levels of liver function and?-foetoprotein(AFP)were recorded。all the factors were put into a logistic regression model for multivariate analysis.Results:the induction achievement ratio of hepatoma model in Wistar rats was 85%.The correct rate of MRI for hepatoma was 94.1%.With multivariate analysis,only 1 of the 7 variables in the final logistic regression model was statistically significant:alanine aminotransferase(ALT).Conclusion:Through giving rats low doses of DENA interruptedly and performing MRI for inspecting pathological changes of rat livers with anomaly increased levels of plasma ALT,we can implement early diagnosis for the hepatom model to decrease the rate of death and extrahepatic metastasis when fund and time is limited.