RESUMO
The Chinese folk medicinal herb Hypericum japonicum, also known as Tianjihuang or Diercao in Chinese, has been widely used for clearing heat and removing toxin, hemostasis and detumescence, and for the treatment of acute and chronic hepatitis, and gastrointestinal disorders. In recent decades, several main types of chemical constituents such as phloroglucinols, flavonoids, xanthonoids, pyranones, and dipeptides have been reported. Modern pharmacology studies have shown that the extracts or secondary metabolites of the herb possess a variety of bio-activities such as hepatoprotective, antioxidant, anti-tumorous, antiviral, anti-bacterial, and anti-malarial activities as well as effects on cardiovascular diseases. In this paper, we presented the main metabolites and pharmacological activities of the plant in recent 30 years, which should be helpful for the further development and utilization of the herb resources of H. japonicum.
RESUMO
Objective:To observe the resistance of hypericum japonicum Thunb.to helicobacter pylori.Methods:Liquid dilution method was used to culture mixture of hypericum japonicum Thunb.extractum and helicobacter pylori,hypericum japonicum Thunb.extraction and helicobacter pylori respectively.The minimum inhibitory concentration and minimal bactericidal concentration of hypericum japonicum Thunb.extractum and extraction were determined by comparing the growth condition of helicobacter pylori.Results:Both of hypericum japonicum Thunb.extractum and extraction had obvious resistant effect on helicobacter pylori.Conclusion:25 mg/ml hypericum japonicum Thunb.extractum and 6.25 mg crude drug/ml hypericum japonicum Thunb.extraction can suppress the growth of helicobacter pylori effectively.
RESUMO
OBJECTIVE:To establish a HPLC method for the simultaneous determination of quercitrin and isoquercetin in hypericum japonicum thunb.injection.METHODS:Merck Lichrospher RP 18 was used as chromatographic column and the mo-bile phase consisted of acetonitrile-(pH=3.0)buffer phosphate(18∶82)with flow rate at1ml/min,column temperature at25℃and detection wavelength at350nm.RESULTS:The linear range for quercitrin and isoquercetin were0.278?g～4.440?g(r=0.9991)and0.315?g～2.520?g(r=0.9991),respectively.The average recovery were98.60%(RSD=2.23%)and97.79%(RSD=1.74%),respectively.CONCLUSION:This method is accurate,reliable and applicable,and suitable for the quality control of hypericum japonicum thunb.injection.