Effects of Kangliyin on N Gene Relative Expression in the Infectious Bronchitis Virus Infected Model / 中药新药与临床药理

Objective To observe the therapeutic effect of Kangliyin(KLY,the compound Chinese herbal formulation with the functions of clearing heat and damp elimination,regulating stomach and dissipate phlegm) on the models with infection of infectious bronchitis virus(IBV),and to evaluate its effect on the IBV N gene relative expression by using real-time florescent quantitative polymerase chain reaction(PCR).Methods Seven-day-old chicks were inoculated with IBV by the nasal and ocular administration.The chicks were divided into 6 groups by stratified random sampling according to the body weight.The 6 groups were blank control group,model group,three KLY treatment groups(receiving KLY at the dosage of 10 g/kg? d-1,20 g/kg? d-1 or 40 g/kg? d-1,respectively),and positive control group(ganciclovir at the dosage of 0.004 g/kg? d-1).The chicks in the model group and blank control group received normal saline,and the medication groups received the corresponding medicine according to the experimental design from the next day of inoculation,and the treatment lasted 5 days.The relative quantity of IBV N gene expression in the lung and bronchial tissues were assayed by real-time florescent quantitative PCR,and the clinical manifestations and the pathological changes of all groups were also examined.Results Compared with the model group,KLY could significantly inhibit the IBV N gene expression,improve the general clinical symptoms and respiratory symptoms,and improve the pathological changes in the bronchus and lung of the IBV infected model.Conclusion KLY could effectively inhibit the viral replication,repair the pathological damage in the bronchus and lung,and improve the general clinical symptoms in the IBV infected model,which is beneficial to the recovery of the chicks from IBV infection.

Effects of Kangliyin on the levels of serum antibody in the chick model of respiratory coronavirus infection / 中国病理生理杂志

AIM:To study the effects of Kangliyin compound (KLY,the compound formulation of a Chinese herbal medicine has 15 kinds of ingredients,including artemisia apiacea,baical skullcap root,tangerine peel,pinellia tuber,frutus aurantii immaturus,caulis bambusae in taeniis,tuckahoe,the larva of silkworm with botrytis,turmeric,agastache,rhubarb,cicada ecdysis,and so on.) treatment on the infectious bronchitis virus (IBV) infection models and to evaluate its effects on the levels of IBV antibody in the serum. METHODS:Seven-day-old chicks were inoculated with the respiration type of IBV M41 strain. All the chicks were bred in a low temperature and highly moisturized controlled environment. The model group and normal control group were fed with sterile normal saline,while the three treatment groups were administrated with KLY compound. Positive control group was irrigated with ganciclovir. These feeds were lasted for 5 d. The clinical symptoms and the pathological changes of all groups' bronchus and lung tissues were examined. The levels of IBV antibody in the serum in each group were also checked by using ELISA method at the end of the study. RESULTS:Compared with the model group,KLY compound significantly increased the serum titer of IBV antibody in the three treatment groups (P