RESUMO
Lumican is a member of the small leucine-rich proteoglycan family, which is involved in cell processes related to tumorigenesis and development, such as epithelial-mesenchymal transition, cell proliferation, migration, invasion and adhesion. The expression of Lumican in different tumors is positively or negatively correlated with tumor progression, and can be used as a reference for tumor prognosis and efficacy evaluation. Further study of the correlation and potential mechanism between Lumican and tumor therapy resistance can provide new ideas for predicting clinical therapeutic efficacy.
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Objective To investigate the expression of tumor suppressor gene Lumican mRNA in gastric cancer and its role in the development of gastric cancer.Methods The expressions of Lumican mRNA in gastric cancer tissues,tissues near tumor and normal tissues were detected by using reverse transcription polymerase chain reaction(RT-PCR),clinical pathology for those tissues was studied as well.Results The expression absence rates of Lumican mRNA were 42.4%(28/66),15.2%(10/66)and 0 in gastric cancer tissues,tissues near tumor and normal tissues respectively.The expression absence rate of Lumican mRNA in patients with lymph node metastasis was 61.1%,which was significantly higher than that of the patients without lymph node metastasis(20.0%,?2=11.323,P=0.001).The expression absence rate of Lumican mRNA in the gastric cancer at the advanced stages(stages Ⅲ,Ⅳ)was 61.5%,which was significantly higher than that in the gastric cancer at the early stages(stages Ⅰ,Ⅱ,30.0%,?2=6.417,P=0.011).The expression absence rates of Lumican mRNA in the high,moderate and poor differentiation tumors were 38.5%(10/26),38.5%(10/26)and 57.1%(8/14)respectively,the expression absence rate of Lumican mRNA was no significant association with the differentiation degree(?2=1.576,P=0.455).Conclusion The expression absence of tumor suppressor gene Lumican mRNA may play an important role in the tumorgenesis and influences the prognosis of gastric cancer.
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BACKGROUND AND OBJECTIVES: It has been suggested that the formation and growth of nasal polyp require the remodeling of extracellular matrix. Proteoglycans (PGs) are the major components of the extracellular matrix that maintain the integrity of the structural tissues The leucine-rich repeat PGs include lumican, decorin and biglycan, all of which have many important biologic activities in various pathologic conditions, including the remodeling of the extracellular matrix. Therefore, these small-PG families may be involved in the formation and growth of nasal polyp. MATERIALS AND METHODS: Surgical specimens of nasal polyps and the normal nasal mucosa were assessed for mRNA expressions coding for lumican, decorin and biglycan using the reverse transcription-polymerase chain reaction,which was followed by dot blot hybridization. RESULTS: Lumican, decorin and biglycan mRNAs were expressed in all tissue samples examined. Semi-quantitative dot blot hybridization revealed that the levels of the lumican and biglycan messages are lower in the nasal polyp tissues than in the nasal mucosa. The decorin messages in the nasal polyp were expressed at levels similar to those in the nasal mucosa. CONCLUSION: These results suggest that lumican, decorin and biglycan may be important components of the extracellular matrix in the nasal mucosa. Considering the function of these PGs, the normal levels of decorin associated with low levels of biglycan and lumican may play a role in the pathogenesis of nasal polyposis.