Development and implementation of multiple mini-stations interview (MMI) to link with the pre-med phase and basic medical learning phase for 8-year program of clinical medicine / 基础医学与临床

Objective_To guide students to better adapt to the life of medical learning phase, and to plan a good profession design, while eliminate candidates do not meet the characteristics of medical school.Methods_Develop and Implement of seven stations of multiple mini-interview in 3-grave medical students.Results_The score of each station shows a normal distribute, and reliability Cronbach’sαcoefficient is 0.629.Conclusions_The design and implementation of multiple mini-interview f to link with the pre-med phase and Basic medical learning phase for 8-year program of clinical medicine is successful, and meet the purpose of ‘ examination promotes learning’ .

Effects of MMI-166 on apoptosis and apoptosis-related protein expression in human pancreatic cancer SW1990 cells / 中华胰腺病杂志

Objective To investigate the effects of MMI-166 on apoptosis and apoptosis-related protein expression of human pancreatic cancer SW1990 cells and its transplanted tumor,and explore possible mechanism.Methods The human pancreatic cancer xenograft model was constructed by using human pancreatic cancer SW1990 cells.Tumor-bearing nude mice were randomly divided into control and MMI-166 groups,and they were treated with normal saline or MMI-166 (200 mg · kg-1 · d-1) for 28 days.Apoptosis index (AI),p53,c-Myc,Bax,Bcl-2,Survivin,Caspase-1,Fas proteins were detected by deoxynucl-eotidyl transferase-mediated nick end labeling (TUNEL method) and Western blot.MMI-166 of different concentrations (0,50,100 μg/ml) were used to treat human pancreatic cancer SW1990 cell for 24 h.The c-Myc,Survivin proteins expressions were measured by Western blotting.Results Apoptosis index in MMI-166 group was 81.1 ±7.9,which was significantly higher than that in control group (21.3 ±2.2,P =0.000) ; the expressions of c-Myc,Survivin were 7715 ± 2229,4594 ± 1240,which were significantly higher than those in control group (16870 ± 2446,15208 ± 1903,P =0.000) ; the expressions of p53,Bax,Bcl-2,Caspase-1,Fas were not significantly different from those in control group.After 50,100 μg/ml MMI-166 treatment,the expression of c-Myc was significantly down-regulated (0.098 ± 0.003,0.073 ± 0.008 vs.0.169 ± 0.007,F =189.361,P ＜ 0.05) ; and the expression of Survivin was not significantly changed.Conclusions MMI-166 may induce cell apoptosis of SW1990 by down-regulating the expression of c-Myc.