RESUMO
Objective: To establish HPLC fingerprints of Qiwei Tangmaishu tablet and simultaneously determine the contents of the six constituents, acteoside, martynoside, calycosin 7-O-β- D-glucopyranoside, schisandrin, formononetin and deoxyschizan- drin. Methods: The analysis of 50% methanol extract of this drug was performed on a 30℃ thermostatic Waters Symmetry C18 column, with the mobile phase comprising of acetonitrile(A)-0.2% formic acid solution(B)flowing at 1.0 ml/min in a gradient elution manner. The detection wavelength was set at 330 nm for acteoside and martynoside, 254 nm for calycosin 7-O-β-D-glucopyranoside, schisandrin, formononetin and deoxyschizandrin. Results: There were thirteen common peaks in the fingerprints of ten batches of samples with the similarities more than 0.9. Acteoside, martynoside, calycosin 7-O-β-D-glucopyranoside, schisandrin, formononetin and deoxyschizandrin showed good linear relationship within the ranges of 1.47-36.75, 0.66-16.50, 0.89-22.25, 2.58-64.50, 1.91-47.75 and 0.77-19.25 μg/ml(r≥0.9993), and their average recoveries were 97.57%, 99.22%, 96.69%, 100.01%, 98.79% and 96.77%, with the RSD of 0.79%, 1.54%, 0.61%, 0.64%, 0.83% and 0.36%, respectively. Conclusion: The established method is easily operable and repeatable, which could be used for quality control of Qiwei Tangmaishu tablet.
RESUMO
Objective: To investigate the chemical constituents from the whole plant of Incarvillea sinensis var. przewalskii, and a systematically phytochemical investigation was carried out. Methods: The chemical constituents were isolated by column chromatographies, such as silica gel and Sephadex LH-20 columns as well as semi-preparative HPLC, and their structures were determined on the basis of MS, NMR spectroscopic analyses, and/or comparison with literature data. Results: Thirteen compounds, including alkaloids and phenylethanoid glycosides, were isolated from the 80% ethanol extract of the whole plants. Their structures were elucidated as p-butoxyl-salidroside (1), incasine C (2), incarvillateine (3), 7-hydroxyskytanthine (4), incarvilline (5), phenethylalcohol glucoside (6), martynoside (7), acteoside (8), deacylisomartynoside (9), decaffeoy1acteoside (10), carceorioside B (11), salidroside (12), and deacylacteoside dimethyl ether (13). Conclusion: Compound 1 is a new one, and the other compounds are isolated from this species for the first time.
RESUMO
Objective: To investigate the chemical constituents from the roots of Pedicularis henryi. Methods: The compounds were isolated by the combined separation Sephadex LH-20, RP18, MCI-gel CHP-20P, and semipreparative HPLC column chromatography. The structures were identified on the basis of NMR data and the comparison with literatures. Results: Nine compounds were isolated from the 95% ethanol extract of P. henryi, and identified as syringaresinol mono-β-D-glucoside (1), plantarenaloside (2), phenylacetic acid (3), 2″, 3″-O-acetylmartynoside (4), cis-2″, 3″-O-acetylmartynoside (5), martynoside (6), cis-martynoside (7), leucoseceptoside A (8), and jionoside D (9). Conclusion: Compounds 1-9 are isolated from this species for the first time.