Discussion on "Method of Strengthening Qi" in the Treatment of Minimal Residual Leukemia / 国际中医中药杂志

The article tries to discuss the usage of "modthod of strengthening qi" in treating minimal residual leukemia(MRL). We believe that the mechanisms for MRI are qi weakness and pathogenic factors staying in yinfen. By using the method of strengthening qi, we hope to achieve the effects of regulating yin and yang, qi and blood, and physiological functions of visceras and meridians, and enhance body immunity. Thus, we can prevent and treat MRL and resist tumor indirectly.

Quantitative detection of minimal residual leukemia cells in peripheral blood and clinical application / 吉林大学学报(医学版)

Objective To explore the clinical application significance of quantitative detection of minimal residual leukemia cells in peripheral blood in acute leukemia patients.Methods Using PCR amplification of quantitative method of limited dilution,the FLT3 gene was detected in blast cells of peripheral blood from 25 newly diagnosed cases of acute leukemia,and the number of minimal residual leukemia cells in newly diagnosed cases and the cases after one course of chemotherapy was calculated respectively.10 healthy subjects were used as control group.Results ①The positive rate of FLT3 gene in 25 newly diagnosed cases of acute leukemia sample was 80%(20/25),the positive rate in acute myeloblastic leukemia(AML) patients was 88.2%(15/17)and in acute lymphocytic leukemia(ALL)was 62.5%(5/ 8).②The mean DNA content in peripheral blood in 20 cases with FLT3 positive expression in newly diagnosed group was(2.36?1.25)?108 ?g?L-1,being equal to(18.66?8.79)?106 leukemia cells in every microliter peripheral blood.After one course of chemotherapy ,there was 1 case without remission,the DNA content in peripheral blood was 1.69?107?g?L-1,being equal to 1.01?106 leukemia cells in every microliter peripheral blood;there were 3 cases with partial remission,the mean DNA content in peripheral blood was(0.57?0.24)?106 ?g?L-1,being equal to(1.82?0.19)?103 leukemia cells in every microliter peripheral blood.There were 9 cases with complete remission with FLT3 negtive expression,and 7 cases with complete remission with FLT3 positive expression,the mean DNA content in peripheral blood was(0.16?0.06)?106 ?g?L-1,being equal to(1.86?1.31)?102 leukemia cells in every microliter peripheral blood.Conclusion The quantitative and periodic detection of minimal residual leukemia cells would help to evaluate leukemia chemotherapy efficiency and to adjust treatment scheme in time.

In vitro protection of Jurkat cells from daunorubicin induced apoptosis by normal bone marrow stromal cells / 中国输血杂志

Objective To study the influence of bone marrow microenvironment on leukemic cells during chemotherapy and the influence on leukemic cells' drug-resistance and anti-apoptosis. Methods Normal bone marrow stromal cells were isolated using Percoll, and then were cocultured with human acute lymphocyte leukemic cell line Jurkat cells in vitro. Apoptosis percentage of Jurkat cells labelled with Annexin V/PI were detected by FCM. Results Apoptosis percentage of Jurkat cells cocultured with bone marrow stromal cells for 24 hours was lower than those maintained in suspension (P