RESUMO
Objective: To study the quality standard of Niaoduqing Tablet, a preparation of Chinese herbal medicine. Methods: Thin layer chromatography (TLC) was used for the identification of Radix et Rhizoma Rhei, Radix Polygoni Multiflori, Radix Sophorae Flavescentis, Radix Salviae Miltiorrhizae and Rhizoma Chuanxiong in Niaoduqing Tablet. TLC scanning was used for the determination of astragaloside IV and HPLC for determination of paeoniflorin in Niaoduqing Tablet. Results: TLC identification were highly specific. A good linearity for astragaloside IV was ranged 1.006～9.064?g and that for paeoniflorin was ranged 0.176～0.880?g. The average recovery of astragaloside IV was 97.69 %( RSD= 2.13 %) and that of paeoniflorin was 97.47 %( RSD=0.87 %). Conclusion: This method can be used for the standard of quality control of Niaoduqing Tablet.
RESUMO
[Objective] To establish a method for the determination of paeoniflorin content in Kun'an Pills. [Methods] High pressure liquid chromatography (HPLC) was used. The chromatographic conditions were: Hypersil ODS column, acetonitrile-water (17 : 83) as mobile phase, detection wavelength at 230 nm and the flow rate being 1.0 mL/min. [Results] A good linearity of paeoniflorin was shown in the range of 0.096 8 - 0.484 0 ?g, r = 0.999 8; the average recovery was 97.84% and the relative standard deviation being 1.44%. [Conclusion] This method is simple and effective and can be used for the quality control for Kun'an Pills.
RESUMO
Objective To establish a proper extraction method for Yangxue Ruanjian Prescription (YRP) by comparing the chemical component contents and analgesic and anti - inflammatory effect of extracts extracted from YRP by different methods. Methods Water - decocting, alcohol - refluxing and alcohol - percolating methods were adopted and component indexes of paeoniflorin and gentiopicrin were determined by HPLC. The anti - inflammatory effect of the extracts were observed on mice with xylol - induced auricular swelling and analgesic effect by hot - plate method. Results The content of gentiopicrin is similar in the extracts extracted by three methods and the content of paeoniflorin is higher in alcohol - percolating extract than in alcohol - refluxing extract, lowest in water - extraction. The analgesic effect of water - decocting extract is better than that of alcohol - refluxing extract, and that of alcohol - percolating extract is weakest; the anti - inflammatory effect of the three extracts is almost the same. Conclusion Water - decocting extraction is suitable to YRP, and this is determined by the characteristics of Chinese compound prescription.
RESUMO
Objective: To establish a HPLC method for the determination of paeoniflorin in Zhuifeng Tougu pill. Methods: HPLC was used with C18 column,CH3OH-H2O(30 :70)as mobile phase and detection wavelength at 230nm. Results: Paeoniflorin showed a good linearity in the range of 21.02～210.20?g. The average recovery was 100.7%and RSD was 2.0%. Conclusion: This method is simple and accurate with good reproducibility, and can be used for the quality control of Zhuifeng Tougu pill.