A randomised study of evaluation of antiulcerogenic activity of quercetin in rats

Background: Peptic ulcer is very common disease. Peptic ulcer results probably due to an imbalance between aggressive factors (acid, pepsin, H. pylori) and defensive factors (gastric mucus, prostaglandins and bicarbonate secretion). Whatever may be the cause of peptic ulcer, it is the gastric acid that prevents ulcer healing and maintain the ulcer. Therefore, most of the drugs available for treatment of peptic ulcer either neutralize the secreted acid or decrease the acid secretion.Methods: The study was conducted on albino rats (Wistar) of 200-250g and maintained under standard conditions (room temperature 24-27oC and humidity 60-65%) with 12h light and dark cycle. The food in the form of dry pellets (Amrut Lab., hyd) and water were available ad libitum. The animal experiments were approved by the ethics committee of the institute.Results: In pyloric ligation model, the rats pre-treated with quercetin showed highly significant protection (p <0.001) when compared to control group and significant protection when compared to ranitidine pre-treated group (p <0.05). In indomethacin model, both quercetin and ranitidine pre-treated groups showed significant protection when compared to control group (p <0.01).Conclusions: In conclusion, it appears that quercetin possess anti-ulcerogenic principles like flavanoids, phenolic compound and caratinoid. These phytoconstituents provides protection against gastric mucosal damage induced by pylorus ligation, aspirin and ethanol, through inhibition of gastric acid, pepsin, histamine and free radical and stimulation of mucus secretion.

Anti-ulcer effect of rhizome of Curcuma longa Linn, by the method of pyloric ligation

Background: Substantial part of the world population has been known for a long time to suffer from peptic ulcer disease. In the present study, Curcuma longa, a plant belonging to the Zingiberaceae family was chosen for investigating its anti-ulcer properties.Methods: The rhizomes of Curcuma longa were collected locally. The extract was prepared by soxhlet extraction with 50% ethanol. Albino rats of Wistar strain (120-200 grams) obtained from the animal house of medical college Thiruvananthapuram were used. Ranitidine was collected from Kerala Sate Drugs and Pharmaceutical LTD Alapuzha. Antiulcer study in rats were done using the method of pyloric ligation.Results: Antiulcer study in rats using the method of pyloric ligation, extract of Curcuma longa in 1000mg/Kg dose levels exhibited significant protection against shay ulceration. The results were comparable to that of standard drug Ranitidine.Conclusions: The present study with extract of Curcuma longa revealed that it has significant anti-ulcer activity.

Preventive effects of Clostridium butyricum on gastric ulceration induced by pylorus ligature in mice / 中国病理生理杂志

[ ABSTRACT] AIM:To investigate the preventive effects of Clostridium butyricum ( C.butyricum) on the type of pylorus ligated gastric ulcer ( GU) in mice and the underlying mechanisms.METHODS:ICR mice were randomly divided into 4 groups:sham operation group, model group, C.butyricum pretreatment group and omeprazole pretreatment group. Gastric pyloric ligation was adopted to establish GU model in mice.The gastric juice was collected to measure the content of gastric free mucus, the pH of gastric juice and the activity of pepsin.The gastric tissues were collected for routine HE stai-ning to observe the pathological changes.The content of glycogen was detected by PAS staining.The protein expression of Bax and Bcl-2 in the gastric mucosa was also assessed by immunohistochemical staining.RESULTS: The HE and PAS staining showed that the C.butyricum pretreatment obviously attenuated the mucosa lesion induced by ligation.Compared with model group, the pH of gastric juice was significantly raised.The activity of pepsin fell off in C.butyricum group, which was lower than that in omeprazole group.In comparison with model group, the content of gastric free mucus was dra-matically increased and PAS staining showed a significant rise in C.butyricum group, but not in omeprazole group.The protein expression of Bax was decreased and the protein expression of Bcl-2 was upgraded in C.butyricum group than those in model group.CONCLUSION:C.butyricum protects gastric mucosa against the challenge of pylorus ligation in mice and its mechanism may be related to inhibiting gastric acid secretion and the activation of pepsin, increasing the production of gastric free mucus, strengthening the expression of bcl-2 gene and inhibiting the expression of bax gene.

Protective effects of left-handed pantoprazole sodium on the rat model of gastric ulcer generated by pyloric ligation / 中国比较医学杂志

Objective The aim of this study was to investigate the effect of left-handed pantoprazole sodium on rat models of gastric ulcer generated by pyloric ligation .Methods The rat models induced by pylorus ligation were treated with a single intravenous injection of left-handed pantoprazole sodium .The gastric ulcer inhibition rate , volume of gastric juice, activity of pepsin, and level of serum gastrin were determined and gastric mucosa tissue was examined by pathology . Results The gastric ulcer index , volume of gastric juice , activity of pepsin and level of serum gastrin in the rats pretreated with left-handed pantoprazole sodium (0.9, 1.8, 3.6 mg/kg, i.v.) were significantly lower than those in the model group .The lesions of gastric mucosa were also improved in the pantoprazole-treated groups .Conclusions Left-handed pantoprazole sodium has remarkable protective effects on the gastric mucosa in rat models of gastric ulcer induced by pyloric ligation.

Gastroprotective and anti-secretory effect of Pep-Up Tablet on pyloric ligation-induced gastric ulcer model in rats.

Aim of the study was to investigate Gastroprotective and anti-secretory effect of Pep-Up Tablet on pyloric ligation-induced gastric ulcer model in rats. Pep-Up Tablet is an Ayurvedic proprietary formulation which is manufactured and marketed by Vasu Healthcare Pvt. Ltd., Vadodara. The selected animals were divided into two groups and each group consisted of six animals. Group-I was considered as disease control and Group-II was as test drug (Pep-Up Tablet) treated group. Pep-Up Tablet (200 mg/kg/day, p.o.) was administered for 7 days by oral route in Group-II. Pep-Up Tablet was studied for its effect on ulcer index, gastric wall secretory parameters and mucin activity. Pretreatment of Pep-Up Tablet showed significant reduction in ulcer index, gastric acid secretion and pepsin activity. Pep-Up Tablet significantly increased mucin activity (TC:TP ratio) as well which was due to significant increase in the total carbohydrate content. From the available data of present study, it can be concluded that Pep-Up Tablet plays important role mainly on inhibition of acid secretion and in increase of mucin secretion which in turn enhances the stability of gastric mucosal barrier. Pep-Up Tablet provided significant gastric cytoprotection against pyloric ligation-induced gastric ulceration in rats.

Effect of Zinc Salts on Acid Secretion and Peptic Ulceration in Albino Rats.

The present study was carried out to see the effect of two zinc salts i.e zinc sulphate and zinc chloride on gastric ulcers induced by stress, pylorus ligation and aspirin in albino rats. The rats were divided into two main groups (zinc sulphate 30, 60, 90 mg/kg i.p and zinc chloride 10 and 20mg/kg i.p). They were further sub-divided into three sub-groups dependant on ulcer model i.e stress, pylorus ligation and aspirin induced ulcers. It was found that zinc sulphate and zinc chloride had a dose dependant reduction in ulcer index in all three models of gastric ulceration. Also, both the salts had anti acid secretory effect, raised pH of gastric secretion and reduced total acidity significantly. Thus zinc salts prevent gastric ulceration. Probably this effect is mediated by anti acid secretory action.

Gastroprotective Effect of Tabernaemontana divaricata (Linn.) R.Br. Flower Methanolic Extract in Wistar Rats.

Tabernaemontana divaricata (L.) R.Br belonging to Apocynaceae family is traditionally used by people in many parts of the world to treat various disorders. The present study was undertaken to investigate anti-ulcer property of Tabernaemontana divaricata flower methanolic extract (TDFME 500 mg/kg, p.o) by pyloric ligation induced gastric ulceration model using Omeprazole (8mg/kg, p.o) as a standard drug in wistar rats. Five parameters i.e., volume of gastric juice, pH, free & total acidities and ulcer index were assessed. The test extract significantly (p< 0.01) decreased volume of gastric juice, free & total acidities and ulcer index. Like standard, it also raised pH of gastric acid. The observed percentage protection for standard and test were 89.84% and 79.53%, respectively. Thus, TDFME 500 mg/kg had a positive effect on all the parameters under study and the results were similar to that of standard. From the above results, it can be concluded that TDFME exhibits remarkable gastroprotective effect.

Evaluation of Cassia singueana extract on stomach HCL production and gastric emptying in rats.

Investigation of the effects of the extract from C. singueana leaves on stomach hydrochloric acid (Hcl) production following histamine administration and on gastric emptying was carried out in rats. Cassia singueana extract (CSE) at 250 and 750 mg/kg significantly (p<0.050) decreased both gastric free-Hcl and total acids as well as the quantity of meal emptied from the stomach when compared with solvent control. The extract at 250 mg/kg exerted a significant decrease in gastric emptying more than cimetidine (100 mg/kg). CSE contains alkaloids, tannins, sterols and terpenes but no flavonoids, saponins, carbohydrates, reducing sugars, starch nor polyuronides.

Protective effect of alanyl-glutamine dipeptide on acute gastric ulcer in rats / 国际药学研究杂志

Objective: To evaluate the protective effect of alanyl-glutamine dipeptide (Ala-Gin) on different acute gastric ulcer models in rats and investigate its possible mechanisms. Methods: The gastric ulcer in rats was induced by waterimmersion restraint stress, ethanol and pylorus ligation. On each gastric ulcer model, sixty SD rats were randomly divided into six groups including the gastric ulcer control group, cimetidine (0.1 g/kg) and marzulene-S (1.0 g/kg) treatment groups,as well as 0.5, 1.0 and 1.5 g/kg Ala-Gin treatment groups. Before the gastric ulcer, different doses of drugs were administered intragastrically,once a day for 3 days. Ulcer index, gastric acid, gastric juice value, gastric acid, free acid, total acid and pepsin activity were used to evaluate and compare the protective effect of Ala-Gin and other anti-ulcer drugs. Results: Ala-Gin significantly reduced the gastric ulcer index in all giving dose (P<0.01) and its protective effect increased significantly with the climbing dose. In all three gastric ulcer models,the anti-ulcer effects of 1.0 and 1.5 g/kg Ala-Gin treatment groups were equal to that of marzulene-S. In addition, Ala-Gin also markedly inhibited the secretion of free acid (P<0.01)and decreased the activity of pepsin (P<0.05) on pylorus ligation gastric ulcer rats. Conclusion: Ala-Gin has obviously anti-ulcer effect on different experimental gastric ulcer models. Except for the known protective effect on gastric mucosa, its anti-ulcer mechanisms may be related to its inhibitory effect on gastric acid and pepsin.