A new flavanoid from Rabdosia rubescens / 中草药

Objective: To study the chemical constituents of Rabdosia rubescens. Methods: The chemical constituent of R. rubescens was separated and purified by using of various column chromatographic technologies (silica gel, MCI, and ODS column chromatography) as well as HPLC. Structures were elucidated by physicochemical characteristics and spectral data. Results: One new flavanoiel compound was isolated from supercritical fluid extract of R. rubescens, and it was elucidated as 3’-hydroxy-2’,4’- dimethoxy-flav-3-ene (1). Conclusion: Compound 1 is a new flavanoid, named as rubescenane A.

Analysis of Volatile Components in Wild Strains of Rabdosia rubescens in Jiyuan Area / 中国药房

OBJECTIVE:To analysis the volatile components in wild strains of Rabdosia rubescensin in Jiyuan area. METH-ODS:Headspace solid phase microextraction (HS-SPME) was conducted to extract the volatile components in 43 wild species of R. rubescens,GC-MS was conducted to identify the components,and peak area normalization method was conducted to calculate the relative content of the components. RESULTS:The variety and relative contents of the volatility components in 43 wild species had obvious differences and the ranges of species number and relative content was from 0 to 11 and from 0 to 84.03% respectively. Caryophyllene,caryophyllene oxide,dihydroactinidiolide,phytone and patchouli alcohol were main components which existed in a variety of different strains of volatile components of R. rubescens,and were mainly composed of terpenes,ketones,alcohols and es-ters. In addition,fatty acids such as palmitic acid,oleic acid and linoleic acid were contained in a few individual varieties. CON-CLUSIONS:The internal quality in 43 wild species of R. rubescens has certain differences.

Study on the Chemical Constituents of Rabdosia rubescens / 中国药房

OBJECTIVE:To study the chemical constituents of Rabdosia rubescens. METHODS:The chemical constituents of R. rubescens were isolated and purified by silica gel,MCI GEL-CHP 20P,Sephadex LH-20 column and recrystallization,and their structure was identified on the base of spectral analysis and physicochemical properties. RESULTS:8 compounds were isolated in R. rubescens and identified as 24ζ-methyl-5α-lanosta-25-ketone(1),maoyecrystal F(2),rosthorin A(3),β-sitosterol(4),daucosterol (5),heptacosanoic acid (6),oridonin (7) and ponicidin (8). CONCLUSIONS:Compound 1 is isolated from R. genera for the first time and compound 3,6 are isolated from this plant for the first time. The research lays a certain foundation for the quality evaluation of R.rubescens.

Antibacterial Activity of Compounds from Rabdosia rubescens / 世界科学技术-中医药现代化

This study was aimed to assay the antibacterial activity of compounds from Rabdosia rubescens. Disc dif-fusion (K-B method) was used to screen the in vitro antibacterial activity. All kinds of chromatography were used to isolate the chemical constituent and structure was identified by MS and NMR spectroscopy. The results showed that six compounds were isolated and identified as oridonin (1), rosmarinic acid (2), caffeic acid (3), salicylic acid (4), ferulic acid (5), vanillic acid (6). Oridonin had activity against Staphylococcus aureus (SA), Methcillin-resistant Staphylococcus aureus (MRSA), β-lactamase positive Staphylococcus aureus (ESBLs-SA), and showed the highest ac-tivity (MIC is 3.125, 6.25 and 6.25 μg·disc-1, respectively), but was still weaker than that of berberine as positive control (MIC is 0.156 μg·disc-1). Ferulic acid had activity against SA and MRSA (MIC is 50 and 50 μg·disc-1, re-spectively). Salicylic acid had only activity against SA (MIC 50 μg·disc-1). It was concluded that oridonin, ferulic acid and salicylic acid were the main antibacterial activity compounds from R. rubescens.

A New Asymmetric ent-Kauranoid Dimer from Rabdosia rubescens / 中草药·英文版

ObjectiveTo study the ent-kaurane diterpenoids from Rabdosia rubescens.MethodsThe compounds were isolated by chromatographies and their structures were identified by spectral analyses.ResultsFour compounds were isolated,and they were identified as bisrubescensin E (1),2α,3α,24-trihydroxyurs-12-en-28-oic acid (2),2α,3α,24-trihydroxyurs-12,20-(30)-dien-28-oic acid (3),and 6,7-dihydroxycoumarin (4).ConclusionCompound 1 is a new asymmetric ent-kauranoid dimer.Compound 2 is isolated from the plant for the first time.Compounds 3 and 4 are isolated from the plants ofRabdosia (B1.) Hassk for the first time.

Efficacy of Rabdosia Rubescens in the Treatment of Gingivitis / 华中科技大学学报（医学）（英德文版）

This study evaluated the efficacy ofrabdosia rubescens against gingivitis and compared the therapeutic efficacy of different dosage forms of rabdosia rubescens. A multi-center, randomized, dou-ble-blind, double-simulation, positive-controlled and parallel trial was conducted. A total of 136 patients exhibiting clinical symptoms of gingivitis were enrolled. The subjects were randomly assigned to two groups: test group (n=67), in which rabdosia rubescens drop pill (960 mg) and 4 tablets of simulation agent of rabdosia rubescen were orally given to the subjects three times a day for 5 days; and control group (n=69), in which the subjects were administered the tablets of rabdosia rubescens (1000 mg) and 24 drop pills of simulation agent of rabdosia rubescens thrice daily for 5 days. The experimental proto-cols and diagnostic criteria were established by expert panel prior to the experiment. The clinical symp-toms were graded according to the severity of the disease and quantified. The total scores and scores for each clinical symptom of gingivitis were assessed at baseline and on the 6th day post-treatment. The therapeutic efficacy was compared between the two groups and in each group itself before and after the treatment. The results showed that in the two groups, the subjects who were given rabdosia rubescens,drop pill or tablet, had a decrease in total scores and scores for each clinical symptom when compared with those before treatment (P<0.01). There was significant difference in the therapeutic efficacy be-tween the test group and the control group with the efficacy rate being 92.54% and 79.71% respectively (P<0.05). It was concluded that rabdosia rubescens showed great promise in treating gingivitis. And rabdosia rubescens drop pill was more efficacious than rabdosia rubescens tablet.

Antitumor and immunological activities of polysaccharides from rabdosia rubescens / 中国病理生理杂志

AIM: To investigate the antitumor and immunological activities of the polysaccharides obtained from rabdosia rubescens (RRP). METHODS: In vitro and in vivo tumor inhibitory experiments as well as MTT method were used. RESULTS: With increasing concentration, the RRP exerted direct cytotoxicity effect in vitro on the cell growth of EAC mice,and its inhibitory rate in vivo against sarcoma 180 reached 35% ( P