Production of Herbal Protein Isolates with the Enzymatic Hydrolysis Technology

The article aims to develop the technology of the production of herbal protein isolates with 41% proteinconcentration by enzymatic hydrolysis. The technology includes preparing a hydro module from shredded lupineseeds and water in proportion 1:10, hydrolysis of starch with alpha-amylase and glucoamylase, centrifugation,autoclaving of the obtained centrifugate at the temperature of 120-130º С and the pressure of 6х105 Pa for 5-6hours, cooling it to the temperature of 36º С and hydrolyzing it with trypsin solution in phosphate buffer solutionat pH 7.5 for 50-60 minutes, centrifugation, heating and drying at the temperature of 95-100 ºС to get the dryresidue concentration of 45% in the protein preparation. Before adding trypsin, it is intensified by blue spectrumlight with the luminous flux of 35 µW/cm2. Based on the research, there are regulated quality indicators of proteinpreparation, storage requirements, and retention periods: retention period of 9 months at the temperature of 0-4°С with relative humidity not more than 75%.

Post-treatment stability of the occlusal plane according to different vertical facial patterns / 대한치과교정학회지

OBJECTIVE: The purpose of this study was to find changes in the occlusal plane related to different vertical facial patterns and suggest treatment goals and conduct possible treatment mechanisms. METHODS: 60 adult patients (28 males, 32 females) who had been diagnosed as Class I skeletal malocclusion and treated without extraction were selected. Patients were divided into three groups; short face type (group 1), average face type (group 2) and long face type (group 3), using the data on normal occlusion of Korean adults. RESULTS: The results were achieved by analyzing cephalometric tracings of each group at pre-treatment, end-treatment and post-treatment (about 1 year recall check). The inclination of the occlusion plane tends to gradually increase as the face becomes longer. In group 1, COP-X, FOP-X, L6/L1, MP-L6 were significantly decreased, and L1-FOP was significantly increased during the retention period (T3-T2). Group 2 showed no significant change. In group 3, FOP-X was significantly increased during the retention period (T3-T2). During the retention period, FOP-X showed significant change among each group, especially between group 1 and group 3. CONCLUSION: These results suggest that changes of occlusal plane inclination according to facial vertical pattern need to be considered during the retention period for intrusion, extrusion, and incisor overbite.

A study on the skeletal changes after treatment of Class III malccusion patients / 대한치과교정학회지

This study was investigated the changes during treatment and retention period in the Class III malocclusion patients and explored the correlationship between factors that showed relapse tendencies and pre-treatment skeletal pattern and the changes during treatment period. Numbers of total sample were 24 and their Hellman's dental age at the start of treatment was over III B and were retained at least over 1 year 6 months. The following conclusion were obtained by comparing the differences between treatment period and retention period, and after analysing the correlationship of factors that manifested relapse tendencies. 1. The angles formed by FH plane and occlusal plane, FH plane and mandibular plane, and mandibular incisor and mandibular plane changes showed rebound effect during retention period and among them occlusal plane angle and IMPA show reverse correlationship. 2. Upward displacement of the occlusal plane at the end of treatment has returning tendency, is proportional to the displacement during treatment period, but the angle between maxillary and mandibular 1st molar to its basal bone have been constantlsy maintained during the retention period. 3. Mandibular plane decrease during retention period and downward backward rotation during treatment period show correlationship.