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Objective To explore the changes of interleukin-4,8 and TPK in PC_(12) cells exposed to hypoxia and soman intoxication.Methods PC_(12) cells were randomized into 4 groups: control,hypoxia only,soman only,hypoxia plus soman.The activity of IL-4,IL-8 was measured by ELISA and the activity of tyrosine protein kinase in cells were detected by RIA.Results The activity of IL-4,IL-8 in PC_(12) cell supernatant and TPK in PC_(12) cell cytoplasm,nucleus were increased in 2 h and reached the highest at 12 h,decreased at 24 h in soman group,correspondingly the activity of IL-4,IL-8 in PC_(12) cell supernatant and TPK in PC_(12) cell cytoplasm,nucleus were increased at 2 h and reached the highest at 6 h,decreased at 12 h in soman plus hypoxia group,all higher than that of control.Conclusion TPK,cytokine and its correlated signal pathway lead to an important modulatory role in the brain damage after combined soman and hypoxia injury.
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Objective To compare the effects of different intervals of theta burst stimulation(TBS) on the expression of long-term potentiation(LTP) and to explore whether LTP is tyrosine protein kinase(TPK)-dependent.in basolateral amygdala(BLA).Methods Basolateral amygdala slices of rats were prepared.Field excitatory post-synaptic potentials(field potential,fEPSPs) of BLA were recorded by stimulating the external capsule.Two TBS's were applied to induce LTP in BLA.Each TBS included a brief,high-frequency pulse train(5 pulses at 100 Hz) given at the theta-rhythm(5Hz) for 4 seconds.Experiments compare the effects of different intervals of two TBS's on the expression of LTP in BLA.The role of tyrosine protein kinase(TPK) on LTP was then determined using bath application of TPK inhibitor genistein.Results Two TBS's of 10 seconds interval failed to induce LTP in BLA.However,two TBS's increased to 10 min and 30 min intervals,individually,both types of stimulations enhanced f-EPSPs.The enhanced f-EPSPs lasted for more than 30 min.LTP induced by two TBS's of 10 min and 30 min interval were blocked by the TPK inhibitor genistein.Conclusion Two TBS's of 10 min intervals was better at the induction of LTP in BLA.The activation of TPK was possibly involved in the induction and maintenance of LTP in the amygdala.
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The effect of Sepia on the activity of tyrosine protein kinase (TPK) protein kinase C (PKC) and cAMP-dependent protein kinase (PKA) was studied in H 22 cancer cells by using the incorporation of ? 32 P ATP into exogenous substract.The results showed that membranous TPK activity of membrane,cytosol and mitochondria was elevated significantly,while the membranous TPK activity was increased obviously;while the cytosolic PKC activity was increased most.The cytosolic PKA activity was increased.These results suggested that Sepia can changeover the activity of TPK,PKC and PKA,which was abnormally changed by cancer cells,then caused that the up regulation was diminished and the down regulation was enhanced towards the Ras MAPK signal transduction pathways.This inhibited or interrupted the cascade transduction of the Ras MAPK pathway,thus produced the effect of anticancer and accelerated the differentiation by transforming cancer cells from undifferentiated proliferation to differentiated proliferation.